Results 81 to 90 of about 20,634,842 (205)

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, Volume 101, Issue 4, Page 1662-1682, August 2026.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

AMB Express, 2019
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda   +7 more
doaj   +1 more source

The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product

Journal of Virological Methods, 2004
High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS-CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZalphaA. SDS-PAGE and Western blotting
Han, Xueqing, Bartlam, Mark, Jin, Ying-hua, Liu, Xiangtao, He, Xiaojing, Cai, Xuepeng, Xie, Qingqe, Rao, Zihe   +7 more
openaire   +2 more sources

Dose‐dependent structural and electron‐density features in the lytic polysaccharide monooxygenase NcAA9D

Acta Crystallographica Section D, Volume 82, Issue 8, Page 900-914, August 2026.
A series of 36 structures of the Neurospora crassa LPMO NcAA9D were refined against X‐ray crystal datasets collected with increasing dose from a single crystal to provide insights into dose‐dependent structural changes at the active site of the enzyme.
Samuel A. Miller, William B. O'Dell, Flora Meilleur   +2 more
wiley   +1 more source

Large-scale functional expression of WT and truncated human adenosine A_2A receptor in Pichia pastoris bioreactor cultures

Microbial Cell Factories, 2008
Background The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host.
Strange Philip G, Rana Rohini, Martinez Magdalena, Ruprecht Jonathan, Fiez-Vandal Cédric, Gras Adrien, Singh Shweta, Wagner Renaud, Byrne Bernadette   +8 more
doaj   +1 more source

Direct oral anticoagulants and warfarin in atrial fibrillation patients with cancer by anticoagulation quality

Cancer, Volume 132, Issue 14, 15 July 2026.
Patients with atrial fibrillation (AF) and cancer treated with direct oral anticoagulants (DOACs) had a lower risk of all‐cause mortality, cardiovascular events (CVEs), but not bleeding events, compared to patients treated with vitamin K antagonists (VKAs) with low anticoagulation quality.
Danilo Menichelli, Vito Maria Daniele Cormaci, Gianluca Gazzaniga, Irma Bisceglia, Massimiliano Camilli, Daniela Poli, Emilia Antonucci, Roberto Pola, Paolo Santini, Pasquale Pignatelli, Daniele Pastori, on behalf of the START2 Registry Investigators   +11 more
wiley   +1 more source

Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system

Synthetic and Systems Biotechnology
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential of Pichia pastoris, renowned for its robust NADPH ...
Yi Li, Xiang Wang, Kaidi Chen, Zhoukang Zhuang, Hongting Tang, Tao Yu, Wenbing Cao   +6 more
doaj   +1 more source

Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris

Electronic Journal of Biotechnology, 2016
Background: Human is an essential cellular enzyme that is found in all human cells. As this enzyme is upregulated in cancer cells exceedingly, it is used as a target for cancer chemotherapeutic drug development. As such, producing the in-house enzyme for
Ruo Ping Ang, Leong Sin Teoh, Mooi Kwai Chan, Noorizan Miswan, Boon Yin Khoo   +4 more
doaj   +1 more source

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Scientific Reports, 2020
The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of the receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 ...
Argentinian AntiCovid Consortium
doaj   +1 more source

Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins

Microbial Cell Factories
Background Pichia pastoris ( Komagataella phaffii ) is a promising production host, but the usage of methanol limits its application in the medicine and food industries.
Xiangna Lin, Weiqiu Ding, Shaoyan Zheng, Lian Wu, Xueming Chen, Chunfang Xie, Daling Liu, Dongsheng Yao   +7 more
semanticscholar   +1 more source