Results 81 to 90 of about 20,152,363 (248)

Recombinant keratinases production by P. pastoris X-33.

, 2013
A: Kinetics of keratinase production by P. pastoris pPICZα-kerAwt, pPICZα-kerAopti1 and pPICZα-kerAopti2. B: Comparative expression of kerAwt, kerAopti1 and kerAopti2 in P. pastoris at 96 h.
Zhiqing Huang (13720), Guoquan Han (385328), Ping Zheng (21877), Jie Yu (150626), Jie Gao (10266), Jun He (46556), Daiwen Chen (326144), Bing Yu (237109), Hong Hu (62885), Xiangbing Mao (147316)   +9 more
core   +1 more source

Nanobodies as next‐generation targeting platforms: From discovery technologies to translational biomedicine

VIEW, EarlyView.
Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley   +1 more source

Serum-free cultured meat production by using Pichia pastoris-derived recombinant albumin

iScience
Summary: Cultured meat production involves the growth, differentiation, and food processing of muscle satellite cells. However, its heavy reliance on fetal bovine serum (FBS) significantly increases production costs, which has prompted interest in ...
Ye Rim Kim, Doyoung Hwang, Si Won Jang, Jae Ho Han, Ga Rim Na, Ji Hoon Park, Gyeongho Tae, Seung Moon Park, Hyun Woo Choi   +8 more
doaj   +1 more source

Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants

Plant, Cell &Environment, EarlyView.
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn, Fabian B. Haas, Benjamin G. Chavez, Bernd H. Dreyer, Erik V. Reiss, Anne Ziplys, Heiko Weichert, Saskia Hiltemann, José M. Ugalde, Andreas J. Meyer, John C. D'Auria, Stefan A. Rensing, Jos H. M. Schippers   +12 more
wiley   +1 more source

Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Microbial Cell Factories, 2021
Background Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities.
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos Luna-Flores, Xiaojing Chen, Juhani von Hellens, Robert Speight   +7 more
doaj   +1 more source

Alliinase: A Comprehensive Review of the Pivotal Enzyme in Garlic Chemistry and Its Applications

Food Frontiers, Volume 7, Issue 4, July 2026.
Garlic crude extract was purified to obtain alliinase, which catalyzes alliin hydrolysis to release allicin. The free and immobilized alliinase shows potential applications in food, biotechnology, pharmaceuticals, health products, and biosensors. ABSTRACT Alliinase is a pyridoxal 5′‐phosphate (PLP)‐dependent lyase that serves as the cornerstone of the ...
Zhenlin Han, Tian Ye, Yantian Tang, Guodong Su, Jiazhou Li, Ningyang Li   +5 more
wiley   +1 more source

Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris

Bioscience Journal, 2018
Xylanase can hydrolyze xylan for reducing its anti-nutritional impact and improving nutrient availability, so obtaining suitable xylanase to degrade xylan is essential.
Shuli Li, Weiwei Huang, Juan Chang, Ping Wang, Qingqiang Yin, Chaoqi Liu, Erzhu Wang, Fushan Lu   +7 more
doaj   +3 more sources

Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris

Asian Pacific Journal of Tropical Biomedicine, 2017
Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris). Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the
Fernita Puspasari, Riski Dwimalida Putri, Aisyah, Raden Roro Rika Damayanti, Anita Yuwita, Bachti Alisjahbana, Sukwan Handali, Ihsanawati, Dessy Natalia   +8 more
doaj   +1 more source

Fendioxypyracil, a new and systemic PPO‐inhibiting herbicide for X‐spectrum weed control

Pest Management Science, Volume 82, Issue 7, Page 6794-6807, July 2026.
This graphical abstract presents the discovery and synthesis of PPO herbicide structures with a central pyridine core, showing molecular conformations, dose–response inhibition curves for PPO1 and PPO2, and comparative weed and grass control efficacy of fendioxypyracil versus other herbicides in greenhouse and field trials.
Tobias Seiser, Aimone Porri, Philipp Johnen, Silke Zeyer, Judith Wahrheit, Michael Betz, Breght Vandenberghe, Scott Asher, Liliana Parra Rapado   +8 more
wiley   +1 more source

The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product

Journal of Virological Methods, 2004
High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS-CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZalphaA. SDS-PAGE and Western blotting
Han, Xueqing, Bartlam, Mark, Jin, Ying-hua, Liu, Xiangtao, He, Xiaojing, Cai, Xuepeng, Xie, Qingqe, Rao, Zihe   +7 more
openaire   +2 more sources