Results 81 to 90 of about 25,435,117 (220)

Large-scale functional expression of WT and truncated human adenosine A_2A receptor in Pichia pastoris bioreactor cultures

Microbial Cell Factories, 2008
Background The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host.
Strange Philip G, Rana Rohini, Martinez Magdalena, Ruprecht Jonathan, Fiez-Vandal Cédric, Gras Adrien, Singh Shweta, Wagner Renaud, Byrne Bernadette   +8 more
doaj   +1 more source

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, Volume 123, Issue 11, Page 2906-2936, November 2026.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

AMB Express, 2019
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda   +7 more
doaj   +1 more source

Behavioural manipulation of the cabbage stem flea beetle (Psylliodes chrysocephala L): Prospects for push–pull strategies in oilseed rape

Pest Management Science, Volume 82, Issue 11, Page 10551-10560, November 2026.
Behavioural manipulation of Psylliodes chrysocephala provides promising opportunities for sustainable pest management in oilseed rape systems. This review synthesises evidence on glucosinolates and their volatile isothiocyanate derivatives as phagostimulants and attractants, alongside the repellent or antifeedant effects of non‐host plants, plant ...
Suleiman Mustapha, Patricia A Ortega‐Ramos, Jozsef Vuts, Tariq M Butt, E. Joel Loveridge, Daniel Rüde, Samantha M Cook   +6 more
wiley   +1 more source

Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system

Synthetic and Systems Biotechnology
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential of Pichia pastoris, renowned for its robust NADPH ...
Yi Li, Xiang Wang, Kaidi Chen, Zhoukang Zhuang, Hongting Tang, Tao Yu, Wenbing Cao   +6 more
doaj   +1 more source

Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris

Electronic Journal of Biotechnology, 2016
Background: Human is an essential cellular enzyme that is found in all human cells. As this enzyme is upregulated in cancer cells exceedingly, it is used as a target for cancer chemotherapeutic drug development. As such, producing the in-house enzyme for
Ruo Ping Ang, Leong Sin Teoh, Mooi Kwai Chan, Noorizan Miswan, Boon Yin Khoo   +4 more
doaj   +1 more source

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Scientific Reports, 2020
The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of the receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 ...
Argentinian AntiCovid Consortium
doaj   +1 more source

Epidemiological drivers of turnip yellows virus and its vector Myzus persicae in Australian canola (Brassica napus) systems: a 3‐year study of commercial crops and noncrop vegetation

Pest Management Science, Volume 82, Issue 11, Page 10671-10682, November 2026.
A 3‐year study across southern Australia aimed to characterize drivers of infection in the Myzus persicae–turnip yellows virus–Brassica napus pathosystem. Identification of key reservoir hosts and the strong association between earlier sowing and increased disease incidence highlight opportunities for targeted weed management, strategic sowing ...
Samantha E Edley, Owain R Edwards, Benjamin S Congdon, John G Black, Marielle Babineau, Anthony R van Rooyen, Alex Slavenko, Roger AC Jones, Andrew R Weeks, Paul A Umina   +9 more
wiley   +1 more source

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

PLoS ONE, 2011
Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent drug efflux pump involved in multidrug resistance of ...
Jiangping Bai, Douglas J Swartz, Irina I Protasevich, Christie G Brouillette, Patina M Harrell, Ellen Hildebrandt, Brigitte Gasser, Diethard Mattanovich, Andrew Ward, Geoffrey Chang, Ina L Urbatsch   +10 more
openaire   +4 more sources

Rational Engineering of Cellobiose Dehydrogenase via Substrate Pocket Redesign and Electron Transfer Optimization for Efficient Bioelectrosynthesis of Lactobionic Acid

ChemElectroChem, Volume 13, Issue 20, 16 October 2026.
Structure‐guided substrate‐pocket engineering and electron‐transfer optimization are integrated to enhance cellobiose dehydrogenase for lactose oxidation. The N313D mutation strengthens substrate‐binding interactions, and A165C and W316H improve electron transfer.
Xinyu Cui, Haiyan Song, Yuanming Wang, Zepeng Kang, Hao Su, Lingling Zhang, Zhiguang Zhu   +6 more
wiley   +1 more source