Results 81 to 90 of about 23,995,070 (219)

Gene expression read counts for Capsella bursa-pastoris

, 2016
This file contains expression values obtained using RNA-seq data for 24 accessions of Capsella bursa-pastoris. Read counting was performed using htseq-count program from HTSeq software v0.6.1 (Anders et al. 2014).
Karl Holm (3300639), Chen, Jun, Jun Chen (4238), Glémin, Sylvain, Lascoux, Martin, Dmytro Kryvokhyzha (3283938), Cornille, Amandine, Martin Lascoux (201001), Wright, Stephen I., Sylvain Glémin (18118), Stephen I. Wright (49030), Lagercrantz, Ulf, Amandine Cornille (165965), Kryvokhyzha, Dmytro, Holm, Karl, Ulf Lagercrantz (395747)   +15 more
core   +1 more source

Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

AMB Express, 2019
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda   +7 more
doaj   +1 more source

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, Volume 72, Issue 9, September 2026.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

PLoS ONE, 2011
Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent drug efflux pump involved in multidrug resistance of ...
Jiangping Bai, Douglas J Swartz, Irina I Protasevich, Christie G Brouillette, Patina M Harrell, Ellen Hildebrandt, Brigitte Gasser, Diethard Mattanovich, Andrew Ward, Geoffrey Chang, Ina L Urbatsch   +10 more
openaire   +4 more sources

Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system

Synthetic and Systems Biotechnology
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential of Pichia pastoris, renowned for its robust NADPH ...
Yi Li, Xiang Wang, Kaidi Chen, Zhoukang Zhuang, Hongting Tang, Tao Yu, Wenbing Cao   +6 more
doaj   +1 more source

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Scientific Reports, 2020
The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of the receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 ...
Argentinian AntiCovid Consortium
doaj   +1 more source

BRET‐Based Approaches for Ion Channels: Emerging Applications and Future Directions

Archiv der Pharmazie, Volume 359, Issue 9, September 2026.
Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Nadine Kock, Laura Heitzer, Joana Massa, Marvin Taterra, Marcel Bermúdez, Oliver Koch, Matthias Schiedel   +6 more
wiley   +1 more source

The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product

Journal of Virological Methods, 2004
High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS-CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZalphaA. SDS-PAGE and Western blotting
Han, Xueqing, Bartlam, Mark, Jin, Ying-hua, Liu, Xiangtao, He, Xiaojing, Cai, Xuepeng, Xie, Qingqe, Rao, Zihe   +7 more
openaire   +2 more sources

The growth curves of phytase expressing P. pastoris with or without co-fermentation with Endo H-P expressing P. pastoris.

, 2015
(A).The growth curve of phytase- and Endo H-P-expressing P. pastoris during the vegetative growth phase. (B). The growth curve of phytase- and Endo H-P- expressing P. pastoris during the induction phase.
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source

Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris

BioTechniques, 2001
Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of ...
Jon Soderholm, Brooke J. Bevis, Benjamin S. Glick   +2 more
doaj   +1 more source