Results 61 to 70 of about 47,430 (223)

Climate and seed mass drive intraspecific variation in seed longevity in storage

American Journal of Botany, EarlyView.
Abstract Premise Conservation seed banks are essential for ex situ plant conservation, but stored seeds slowly deteriorate and lose viability. Seed longevity in storage is determined by the initial seed viability and the rate of seed viability loss. The rate of seed viability loss in storage varies between species and possibly between populations or ...
Lea Klepka, Sascha Liepelt, Anna Bucharova   +2 more
wiley   +1 more source

Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Synthetic and Systems Biotechnology
Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P.
Shupeng Ruan, Yuxin Yang, Xinying Zhang, Guanjuan Luo, Ying Lin, Shuli Liang   +5 more
doaj   +1 more source

A novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha [PDF]

, 2007
The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products.
Boer, Arjo L. de,, Geerlings, Torsten H.,, Klei, Ida J. van der,, Lunenborg, Marcel G.J.,, Veenhuis, Marten,   +4 more
core   +2 more sources

Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor. [PDF]

, 2017
About 50% of the world's arable land is strongly acidic (pH ≤ 5). The low pH solubilizes root-toxic ionic aluminium (Al3+) species from clay minerals, driving the evolution of counteractive adaptations in cultivated crops.
Chang, Geoffrey, Doshi, Rupak, Garza, Denisse M, Kochian, Leon V, McGrath, Aaron P, Piñeros, Miguel, Szewczyk, Paul   +6 more
core   +3 more sources

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

Microorganisms, 2021
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris ...
Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang   +5 more
doaj   +1 more source

Pengaruh Konsentrasi Penginduksi Metanol Serta"sumber Karbon Sorbitol Dan Monitol Terhadap Produksi A-amilase Saccharomycopsis Fibuligera R64 Dalam Pichia Pastoris [PDF]

, 2011
Pichia pastoris has been widely used as host for heterologous protein expression for commercial purposes. The advantages of P. pastoris as protein expression host is its ability to grow with high cell density and the presence of a gene promoter that ...
Gaffar, S. (Shabarni), Ismayana, S. (Safrl), Meirina, T. N. (Triana), Permana, D. (Dani), Rahmawati, D. P. (Diana), Subroto, T. (Toto), Suprijana, O. (O), Syihab, A. B. (Abu)   +7 more
core   +1 more source

Microbial Cell Factories / Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion [PDF]

, 2014
Background: The methylotrophic yeast Pichia pastoris is frequently used for the production of recombinant proteins. However, expression levels can vary depending on the target protein.
Buchetics, Markus, Gasser, Brigitte, Kotz, Daniela, Mattanovich, Diethard, Naschberger, Stefan, Pichler, Harald, Ruth, Claudia, Vidimce, Viktorija   +7 more
core   +2 more sources

Transfer Learning Approaches in Bioprocess Engineering: Opportunities and Challenges

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Transfer learning (TL) has recently emerged as a promising approach to overcoming one of the key limitations of bioprocess engineering: data scarcity. By leveraging knowledge from one bioprocess to another, TL allows existing models and data sets to be reused efficiently, accelerating process development, improving prediction accuracy, and ...
Daniel Barón Díaz, Anna‐Lena Drommershausen, Alexander Grünberger, Dirk Holtmann   +3 more
wiley   +1 more source

Selective engagement of FcγRIV by a M2e-specific single domain antibody construct protects against influenza A virus infection [PDF]

, 2019
Lower respiratory tract infections, such as infections caused by influenza A viruses, are a constant threat for public health. Antivirals are indispensable to control disease caused by epidemic as well as pandemic influenza A.
Ballegeer, Marlies, Creytens, Sarah, De Vlieger, Dorien, Hengel, Hartmut, Hoffmann, Katja, Nerinckx, Wim, Remaut, Han, Saelens, Xavier, Schepens, Bert, Van Hoecke, Lien, Van Molle, Inge   +10 more
core   +2 more sources

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source