Results 61 to 70 of about 25,435,117 (220)

From knowledge to action: the importance of characterizing herbicide resistance mechanisms for implementing management strategies

Pest Management Science, EarlyView.
This review bridges the gap between understanding resistance mechanisms and applying them in weed management, highlighting how mechanistic insights reveal vulnerabilities that can guide more effective control strategies. Abstract Herbicide resistance remains one of the most intensively studied areas in weed science because of its direct consequences ...
Luan Cutti, Eric L. Patterson, Todd A. Gaines, Aldo Merotto   +3 more
wiley   +1 more source

The amino acid sequence of the HPAMPD cDNA clone expressed in P. pastoris.

, 2023
The amino acid sequence of the HPAMPD cDNA clone expressed in P. pastoris.
Liang Wang (23021), Ira Schildkraut (29742), Cristian Ruse (2449222), George Tzertzinis (154499), Bryce Causey (16625055), Mehul B. Ganatra (11310145), Christopher H. Taron (516779)   +6 more
core   +1 more source

Production of isotope-labeled nanobody KN035 in Pichia pastoris yields native-like conformation and PD-L1 binding activity comparable to KN035 expressed in mammalian cells

Magnetic Resonance Letters
Stable isotope-labeled proteins are indispensable for NMR-based structural studies. E. coli is a cost-effective and high-yield expression host, but it often fails to support proper folding and post-translational modifications required for functional ...
Piaoran Ren, Mengzhen Jiang, Shenlin Wang   +2 more
doaj   +1 more source

Biology, ecology, and conservation of the Italian hare Lepus corsicanus: a review

Wildlife Biology, EarlyView.
The Italian hare Lepus corsicanus, an endemic lagomorph of Italy, serves as a critical case study in evolutionary biology and conservation. Once taxonomically debated, modern genetic and morphological analyses confirm its status as a distinct Pleistocene lineage restricted to the central‐southern Italian Peninsula and Sicily.
Pierangelo Freschi, Valter Trocchi
wiley   +1 more source

A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis

Yeast, EarlyView.
ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley   +1 more source

R scripts: MCMCglmm analyses of Capsella bursa-pastoris

, 2016
These scripts were used to perform correction for population structure in the analyses of gene expression and phenotypic variation in Capsella bursa ...
Karl Holm (3300639), Chen, Jun, Jun Chen (4238), Glémin, Sylvain, Lascoux, Martin, Dmytro Kryvokhyzha (3283938), Cornille, Amandine, Martin Lascoux (201001), Wright, Stephen I., Sylvain Glémin (18118), Stephen I. Wright (49030), Lagercrantz, Ulf, Amandine Cornille (165965), Kryvokhyzha, Dmytro, Holm, Karl, Ulf Lagercrantz (395747)   +15 more
core   +1 more source

Serum-free cultured meat production by using Pichia pastoris-derived recombinant albumin

iScience
Summary: Cultured meat production involves the growth, differentiation, and food processing of muscle satellite cells. However, its heavy reliance on fetal bovine serum (FBS) significantly increases production costs, which has prompted interest in ...
Ye Rim Kim, Doyoung Hwang, Si Won Jang, Jae Ho Han, Ga Rim Na, Ji Hoon Park, Gyeongho Tae, Seung Moon Park, Hyun Woo Choi   +8 more
doaj   +1 more source

Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Microbial Cell Factories, 2021
Background Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities.
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos Luna-Flores, Xiaojing Chen, Juhani von Hellens, Robert Speight   +7 more
doaj   +1 more source

Functional and transcriptomic analyses in Neurospora crassa reveal a crucial role for the N‐glycoprotein deglycosylation process in fungal homeostasis

The FEBS Journal, EarlyView.
In Neurospora crassa, a cytosolic GH18 endo‐β‐n‐acetylglucosaminidase (ENGase) functions as an active deglycosylating enzyme. Deletion of this severely alters tolerance to multiple stress conditions and causes extensive transcriptomic reprogramming, including upregulation of a GH20 exo‐β‐n‐acetylhexosaminidase under ER stress, indicating that N. crassa
Anastasios Samaras, Tanim Jabid Hossain, Magnus Karlsson, Georgios Tzelepis   +3 more
wiley   +1 more source

A cytoplasm to vacuole targeting pathway in P-pastoris [PDF]

, 2007
The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape 1) from the cytosol to the vacuole, bypassing the normal secretary route. The Cvt pathway, although well-studied, was known only in S. cerevisiae.
Farré, Jean-Claude, Subramani, Suresh, Vidal, Jason   +2 more
core