Results 41 to 50 of about 25,435,117 (220)

Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Synthetic and Systems Biotechnology
Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P.
Shupeng Ruan, Yuxin Yang, Xinying Zhang, Guanjuan Luo, Ying Lin, Shuli Liang   +5 more
doaj   +1 more source

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

PLoS ONE, 2011
Metabolic profiling is increasingly being used to investigate a diverse range of biological questions. Due to the rapid turnover of intracellular metabolites it is important to have reliable, reproducible techniques for sampling and sample treatment ...
Gregory D Tredwell, Bryn Edwards-Jones, David J Leak, Jacob G Bundy   +3 more
doaj   +1 more source

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy

Frontiers in Microbiology, 2020
Pichia pastoris (Komagataella phaffii) is a methylotrophic yeast that is widely used in industry as a host system for heterologous protein expression.
Chengbo Zhang, Yu Ma, Huabiao Miao, Xianghua Tang, Xianghua Tang, Xianghua Tang, Xianghua Tang, Bo Xu, Bo Xu, Bo Xu, Bo Xu, Qian Wu, Qian Wu, Qian Wu, Qian Wu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Zunxi Huang, Zunxi Huang, Zunxi Huang, Zunxi Huang   +22 more
doaj   +1 more source

A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

Synthetic and Systems Biotechnology, 2023
The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter (pAOX1). However, it is difficult to use P.
Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu   +6 more
doaj   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Enabling Bioprocess Upscaling Prediction Through Hybrid Modelling and Transfer Learning Under Small‐Data Scenarios

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Accurate prediction of bioprocess scale‐up is critical for accelerating the deployment of novel and sustainable biomanufacturing systems. However, this remains challenging as multi‐scale data is expensive to generate and mechanistic understanding is often incomplete, leading upscaling decisions to rely heavily on empirical expertise. This work
Harvey Al‐Ramadhan, Fernando Vega‐Ramon, Keju Jing, Dongda Zhang   +3 more
wiley   +1 more source

Methanol-based biosynthesis of p-coumaric acid by engineered Pichia pastoris. [PDF]

Bioresour Bioprocess
Abstract p -Coumaric acid (p-CA) is a key aromatic precursor for the biosynthesis of flavonoids, stilbenoids, and other high-value phenylpropanoids. While microbial production of p-CA typically relies on sugar-based substrates, methanol offers a sustainable and cost-effective alternative ...
Chen M, Fang J, Wang S, Liu G, Zhang Y, Li Y, Jia K, Zhu T.   +7 more
europepmc   +2 more sources

Engineered immunostimulatory H2O2‐producing microreactors driven by yeast as localized living cancer therapeutics

BMEMat, EarlyView.
Engineered yeast microreactors that produce H2O2 within tumors enable selective cancer cell killing while releasing immunostimulatory components, effectively suppressing local tumor growth and activating systemic antitumor immunity, with further efficacy enhanced by anti‐PD‐1 therapy and transarterial embolization.
Wenqian He, Chunjie Wang, Minming Chen, Weihao Yang, Bin Yu, Yuhang Hu, Hengze Ding, Yifan Yan, Zhijuan Yang, Liangzhu Feng   +9 more
wiley   +1 more source

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

Microorganisms, 2021
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris ...
Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang   +5 more
doaj   +1 more source

Quantifying Actin Persistence Length in S. pombe and S. cerevisiae: The Impact of Data Size, Filament Length and Fitting Strategy

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments exhibit isoform and species‐specific mechanical properties that are commonly quantified via their persistence length, yet the reliability of such measurements depends not only on the analysis method but also on dataset characteristics.
Peter Nietmann, Andrejus Suchenko, Andreas Janshoff, Mohan K. Balasubramanian   +3 more
wiley   +1 more source