Results 41 to 50 of about 47,430 (223)

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

open access: yesMicrobial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Gonadotropins in the Russian Sturgeon: Their Role in Steroid Secretion and the Effect of Hormonal Treatment on Their Secretion. [PDF]

open access: yes, 2016
In the reproduction process of male and female fish, pituitary derived gonadotropins (GTHs) play a key role. To be able to specifically investigate certain functions of Luteinizing (LH) and Follicle stimulating hormone (FSH) in Russian sturgeon ...
Aizen, Joseph   +8 more
core   +3 more sources

Lack of correlation between constitutive and induced resistance to a herbivore in crucifer plants: real or flawed by experimental methods? [PDF]

open access: yes, 2009
The correlation between constitutive and induced resistance to herbivores in plants has long been of interest to evolutionary biologists, and various approaches to determining levels of resistance have been used in this field of research.
Dicke, M.   +4 more
core   +2 more sources

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

open access: yesMicrobial Cell Factories, 2004
Background Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty.
Porro Danilo   +6 more
doaj   +1 more source

Screening and evaluation of the strong endogenous promoters in Pichia pastoris

open access: yesMicrobial Cell Factories, 2021
Background Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds.
Weiwang Dou   +4 more
doaj   +1 more source

Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax [PDF]

open access: yes, 2015
Sialidases and trans-sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes, act as virulence factors or mediate cellular interactions (cell evasion and invasion).
Ameloot, Paul   +5 more
core   +1 more source

Atg21 regulates pexophagy via its PI(3)P-binding activity inPichia pastoris [PDF]

open access: yesFEMS Yeast Research, 2014
Pexophagy is a selective degradation pathway of peroxisomes. In the present study, we revealed that PpAtg21 was required for pexophagy in the methylotrophic yeast Pichia pastoris. PpAtg21 was essential for efficient lipidation of Atg8 and for de novo synthesis of pexophagic membranes.
Naoki, Tamura   +2 more
openaire   +2 more sources

Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state [PDF]

open access: yes, 2017
Human respiratory syncytial virus (RSV) is the main cause of lower respiratory tract infections in young children. The RSV fusion protein (F) is highly conserved and is the only viral membrane protein that is essential for infection.
Chen, Man   +13 more
core   +2 more sources

Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism [PDF]

open access: yesMicrobial Biotechnology, 2019
Summary Pichia pastoris KM 71H (Mut S ) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP ‐powered efflux pump which is expressed ...
Wan‐cang Liu   +3 more
openaire   +3 more sources

Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs

open access: yesBioresources and Bioprocessing, 2017
Background Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P.
Rui Ye   +7 more
doaj   +1 more source

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