Results 41 to 50 of about 20,634,842 (205)
Genetic tools for metabolic engineering of Pichia pastoris
Engineering Microbiology, 2023 The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins.
Xiaoyan Wu +3 more
semanticscholar +1 more source
Extracellular Production of the Taiwan-Native Norovirus P Domain Overexpressed in Pichia pastoris
Fermentation, 2023 Many efforts in norovirus vaccine development have focused on subunit or recombinant protein vaccines, such as subviral P particles formed by the protruding (P) domain of VP1. P particles are immunogenic and have a region with a human histo-blood group antigen binding site, an interaction critical for infecting the host. In the past, only intracellular
Chien, Man-Ling +2 more
openaire +2 more sources
Nucleic Acids Research, 2021 The industrial yeast Pichia pastoris has been harnessed extensively for production of proteins, and it is attracting attention as a chassis cell factory for production of chemicals.
Peng Cai +5 more
semanticscholar +1 more source
Microbial Cell Factories, 2004 Background Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty.
Porro Danilo +6 more
doaj +1 more source
Screening and evaluation of the strong endogenous promoters in Pichia pastoris
Microbial Cell Factories, 2021 Background Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds.
Weiwang Dou +4 more
doaj +1 more source
International Journal of Molecular Sciences, 2023 α-Farnesene, an acyclic volatile sesquiterpene, plays important roles in aircraft fuel, food flavoring, agriculture, pharmaceutical and chemical industries. Here, by re-creating the NADPH and ATP biosynthetic pathways in Pichia pastoris, we increased the
Shengmin Chen +3 more
semanticscholar +1 more source
Bioresources and Bioprocessing, 2017 Background Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P.
Rui Ye +7 more
doaj +1 more source
Applied Microbiology and Biotechnology, 2023 Aromatic secondary metabolites are widely used in various industries, including the nutraceutical, dietary supplement, and pharmaceutical industries. Their production currently relies on plant extraction.
Ryota Kumokita +4 more
semanticscholar +1 more source
Microbial Cell Factories, 2012 Background Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P ...
Chen Ming-Tang +5 more
doaj +1 more source
Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production
Advanced Science, EarlyView.ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du +4 more
wiley +1 more source