Results 41 to 50 of about 20,152,363 (248)

Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

open access: yesBiotechnology for Biofuels, 2019
Background Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient.
Yide Huang   +5 more
doaj   +1 more source

Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors

open access: yesAMB Express, 2021
The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals.
Barbara G. Paes   +4 more
doaj   +1 more source

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

open access: yesMicrobial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Genetic tools for metabolic engineering of Pichia pastoris

open access: yesEngineering Microbiology, 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins.
Xiaoyan Wu   +3 more
semanticscholar   +1 more source

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

open access: yesMicrobial Cell Factories, 2004
Background Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty.
Porro Danilo   +6 more
doaj   +1 more source

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

open access: yesNucleic Acids Research, 2021
The industrial yeast Pichia pastoris has been harnessed extensively for production of proteins, and it is attracting attention as a chassis cell factory for production of chemicals.
Peng Cai   +5 more
semanticscholar   +1 more source

Screening and evaluation of the strong endogenous promoters in Pichia pastoris

open access: yesMicrobial Cell Factories, 2021
Background Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds.
Weiwang Dou   +4 more
doaj   +1 more source

Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs

open access: yesBioresources and Bioprocessing, 2017
Background Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P.
Rui Ye   +7 more
doaj   +1 more source

Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

open access: yesBiotechnology for Biofuels, 2018
BackgroundInterests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive ...
Wiparat Siripong   +6 more
semanticscholar   +1 more source

Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris

open access: yesApplied Microbiology and Biotechnology, 2023
Aromatic secondary metabolites are widely used in various industries, including the nutraceutical, dietary supplement, and pharmaceutical industries. Their production currently relies on plant extraction.
Ryota Kumokita   +4 more
semanticscholar   +1 more source

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