Results 51 to 60 of about 23,995,070 (219)

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

BMC Biotechnology, 2018
Background The methanol-regulated AOX1 promoter (P AOX1 ) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of P AOX1 restricts
Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong, Ching-Tsan Huang   +3 more
doaj   +1 more source

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

Microorganisms, 2021
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris ...
Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang   +5 more
doaj   +1 more source

Electronic Nose‐Based Methanol Feedback Precision Control Strategy for High‐Yield Alkaline Phosphatase Fermentation by Hansenula polymorpha

Food Bioengineering, EarlyView.
Establishment of Closed‐Loop Methanol Feedback Precision Control System for Alkaline Phosphatase by Hansenula polymorpha. The electronic nose screened model for methanol detection as the core technical logic of online gas quantification. Methanol feedback control strategy for greatly elevating alkaline phosphatase production.
Jian‐Guang Liang, Shao‐Jie Wang, Jia‐Jia Wang, Muhammad Safwan Siddique, Zishu Zhang, Ciying Xiao, Ze‐Jian Wang   +6 more
wiley   +1 more source

Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism [PDF]

Microbial Biotechnology, 2019
Summary Pichia pastoris KM 71H (Mut S ) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP ‐powered efflux pump which is expressed ...
Wan‐cang Liu, Fei Zhou, Di Xia, Joseph Shiloach   +3 more
openaire   +3 more sources

Monitoring and Mitigation Strategies for Hazardous Compounds in Chinese Rice Wine (Huangjiu): A Comprehensive Review

Food Safety and Health, EarlyView.
This review elucidates the formation pathways and detection technologies for ethyl carbamate, biogenic amines, and higher alcohols in Huangjiu. It concludes that integrated control frameworks are essential to decouple safety risks from the metabolic processes responsible for flavor development.
Lulu Liu, Zhiqiang Kong, Lin Li, Yifan Zhang, Bei Fan, Minmin Li   +5 more
wiley   +1 more source

Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris [PDF]

Glycobiology, 2004
N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi.
Robert C, Davidson, Juergen H, Nett, Eduard, Renfer, Huijuan, Li, Terrance A, Stadheim, Benton J, Miller, Robert G, Miele, Stephen R, Hamilton, Byung-Kwon, Choi, Teresa I, Mitchell, Stefan, Wildt   +10 more
openaire   +2 more sources

Binuclear Copper‐Dependent Oxidative Enzymes Involved in Fungal Natural Product Modifications

Journal of the Chinese Chemical Society, EarlyView.
This article summarizes recent biochemical characterizations of a new enzyme family named by the authors as binuclear copper‐dependent oxidative enzymes (BiNCOs). Found in fungal natural product biosynthesis, BiNCOs catalyze diverse CH functionalization reactions, including C(sp3)H halogenation, C(sp3)H hydroxylation, C(sp3)O macrocyclization, and ...
Chen‐Yu Chiang, Masao Ohashi, Yi Tang
wiley   +1 more source

Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Microbial Cell Factories, 2012
Background Lipase from Rhizopus chinensis is a versatile biocatalyst for various bioconversions and has been expressed at high-level in Pichia pastoris. However, the use of R.
Yu Xiao-Wei, Wang Rui, Zhang Meng, Xu Yan, Xiao Rong   +4 more
doaj   +1 more source

Behavioural manipulation of the cabbage stem flea beetle (Psylliodes chrysocephala L): Prospects for push–pull strategies in oilseed rape

Pest Management Science, EarlyView.
Behavioural manipulation of Psylliodes chrysocephala provides promising opportunities for sustainable pest management in oilseed rape systems. This review synthesises evidence on glucosinolates and their volatile isothiocyanate derivatives as phagostimulants and attractants, alongside the repellent or antifeedant effects of non‐host plants, plant ...
Suleiman Mustapha, Patricia A Ortega‐Ramos, Jozsef Vuts, Tariq M Butt, E. Joel Loveridge, Daniel Rüde, Samantha M Cook   +6 more
wiley   +1 more source

From knowledge to action: the importance of characterizing herbicide resistance mechanisms for implementing management strategies

Pest Management Science, EarlyView.
This review bridges the gap between understanding resistance mechanisms and applying them in weed management, highlighting how mechanistic insights reveal vulnerabilities that can guide more effective control strategies. Abstract Herbicide resistance remains one of the most intensively studied areas in weed science because of its direct consequences ...
Luan Cutti, Eric L. Patterson, Todd A. Gaines, Aldo Merotto   +3 more
wiley   +1 more source