Results 51 to 60 of about 20,634,842 (205)

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

PLoS ONE, 2011
Metabolic profiling is increasingly being used to investigate a diverse range of biological questions. Due to the rapid turnover of intracellular metabolites it is important to have reliable, reproducible techniques for sampling and sample treatment ...
Gregory D Tredwell, Bryn Edwards-Jones, David J Leak, Jacob G Bundy   +3 more
doaj   +1 more source

Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Biotechnology for Biofuels, 2018
BackgroundInterests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive ...
Wiparat Siripong, Philip F. Wolf, Theodora Puspowangi Kusumoputri, J. Downes, K. Kocharin, S. Tanapongpipat, Weerawat Runguphan   +6 more
semanticscholar   +1 more source

From Circuits to Symphonies: A Systems‐Engineering Blueprint for Multimicrobial Synthetic Biology

Advanced Science, EarlyView.
Engineering synthetic microbial consortia requires moving beyond intracellular genetic circuits toward coordinated multicellular design. A modular systems‐engineering framework is presented that integrates communication, temporal coordination, ecological structuring, and predictive modeling to guide application‐dependent consortium design ...
Miguel Fernández‐Niño, Ludger A. Wessjohann, José Manuel Guillamón, Daniela Burgos‐Toro, Silvia Moriano‐Gutiérrez, Giacomo Di Matteo, Zia‐ul Islam, Rinke van van Tatenhove‐Pel, Vanessa Rossetto Marcelino, Rodrigo Ledesma‐Amaro   +9 more
wiley   +1 more source

Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Synthetic and Systems Biotechnology
Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P.
Shupeng Ruan, Yuxin Yang, Xinying Zhang, Guanjuan Luo, Ying Lin, Shuli Liang   +5 more
doaj   +1 more source

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy

Frontiers in Microbiology, 2020
Pichia pastoris (Komagataella phaffii) is a methylotrophic yeast that is widely used in industry as a host system for heterologous protein expression.
Chengbo Zhang, Yu Ma, Huabiao Miao, Xianghua Tang, Xianghua Tang, Xianghua Tang, Xianghua Tang, Bo Xu, Bo Xu, Bo Xu, Bo Xu, Qian Wu, Qian Wu, Qian Wu, Qian Wu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Zunxi Huang, Zunxi Huang, Zunxi Huang, Zunxi Huang   +22 more
doaj   +1 more source

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

Applied Microbiology and Biotechnology, 2021
Folding and processing of proteins in the endoplasmic reticulum (ER) are major impediments in the production and secretion of proteins from Pichia pastoris (Komagataella sp.).
Hana Raschmanová, A. Weninger, Z. Knejzlík, K. Melzoch, K. Kovar   +4 more
semanticscholar   +1 more source

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling

Biotechnology and Bioengineering, EarlyView.
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro, José Luis Checa‐Barrera, Juan A. Moreno‐Cid, Eoin Casey   +3 more
wiley   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek, Jean‐Marc Daran, Marieke E. Klijn, Marcel A. Vieira‐Lara   +3 more
wiley   +1 more source

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

Microorganisms, 2021
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris ...
Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang   +5 more
doaj   +1 more source