Results 51 to 60 of about 47,430 (223)

The Pichia pastoris transmembrane protein GT1 is a glycerol transporter and relieves the repression of glycerol on AOX1 expression [PDF]

, 2016
Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris).
Bai, Zhonghu, Dai, Xiaofeng, Harvey, Linda, Liu, Xiuxia, McNeil, Brian, Sun, Yang, Wang, Songwei, Yang, Yankun, Zhan, Chunjun   +8 more
core   +1 more source

Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris [PDF]

Glycobiology, 2004
N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi.
Robert C, Davidson, Juergen H, Nett, Eduard, Renfer, Huijuan, Li, Terrance A, Stadheim, Benton J, Miller, Robert G, Miele, Stephen R, Hamilton, Byung-Kwon, Choi, Teresa I, Mitchell, Stefan, Wildt   +10 more
openaire   +2 more sources

Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1 [PDF]

, 2015
Medium-chain volatile flavour esters are important molecules since they have extensive applications in food, fragrance, cosmetic, paint and coating industries, which determine different characteristics of aroma or taste in commercial products ...
AB Mason, ASG Lorenzoni, B Gao, C Gurkan, D Guo, E Descours, EJ Vandamme, FM Ausubel, G Brault, GM Rodriguez, J Yan, JA Arcos, JF Mosher, JL Cereghino, JR Pringle, LF Salerno, M Ahmad, M Choder, M Madruga, M Matsumoto, MJ Knight, MW Cheong, MW Larsen, NA Zamzuri, P Malcorps, R Noguerol-Pato, S Adachi, S Pokoj, SDM Laere Van, SM Saerens, SM Saerens, SM Saerens, Y Chen, Y Xu, Z Jin, Z Jin   +35 more
core   +3 more sources

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Microbial Cell Factories, 2012
Background Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P ...
Chen Ming-Tang, Lin Song, Shandil Ishaan, Andrews Dewan, Stadheim Terrance A, Choi Byung-Kwon   +5 more
doaj   +1 more source

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

PLoS ONE, 2011
Metabolic profiling is increasingly being used to investigate a diverse range of biological questions. Due to the rapid turnover of intracellular metabolites it is important to have reliable, reproducible techniques for sampling and sample treatment ...
Gregory D Tredwell, Bryn Edwards-Jones, David J Leak, Jacob G Bundy   +3 more
doaj   +1 more source

A comparative study of peroxisomal structures in Hansenula polymorpha pex mutants [PDF]

, 2007
In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis (PEX genes) in various fungi.
Klei, Ida J. van der,, Koek, Anne,, Komori, Masayuki,, Veenhuis, Marten,   +3 more
core   +2 more sources

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy

Frontiers in Microbiology, 2020
Pichia pastoris (Komagataella phaffii) is a methylotrophic yeast that is widely used in industry as a host system for heterologous protein expression.
Chengbo Zhang, Yu Ma, Huabiao Miao, Xianghua Tang, Xianghua Tang, Xianghua Tang, Xianghua Tang, Bo Xu, Bo Xu, Bo Xu, Bo Xu, Qian Wu, Qian Wu, Qian Wu, Qian Wu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Zunxi Huang, Zunxi Huang, Zunxi Huang, Zunxi Huang   +22 more
doaj   +1 more source

A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

Synthetic and Systems Biotechnology, 2023
The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter (pAOX1). However, it is difficult to use P.
Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu   +6 more
doaj   +1 more source

Economic analysis of royalactin production under uncertainty: Evaluating the effect of parameter optimization. [PDF]

, 2015
Royalactin is a protein with several different potential uses in humans. Research, in insects and in mammalian cells, has shown that it can accelerate cell division and prevent apoptosis.
Aguilar-Yañez, JM, Rito-Palomares, M, Titchener-Hooker, NJ, Torres-Acosta, MA   +3 more
core   +1 more source