Results 51 to 60 of about 20,152,363 (248)

Glycosylation of thrombospondin type 1 repeats [PDF]

, 2008
In the course of mutagenesis of the forth thrombospondin type 1 repeat (TSR4) of the axonal guidance protein F-spondin, one mutant designated ΔDS2,3-TSR4 missing two out of three cystein bridges was found to lack the disaccharide Glcβ1,3-Fuc-O.
Krantz, Carsten
core   +1 more source

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Microbial Cell Factories, 2012
Background Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P ...
Chen Ming-Tang, Lin Song, Shandil Ishaan, Andrews Dewan, Stadheim Terrance A, Choi Byung-Kwon   +5 more
doaj   +1 more source

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

PLoS ONE, 2011
Metabolic profiling is increasingly being used to investigate a diverse range of biological questions. Due to the rapid turnover of intracellular metabolites it is important to have reliable, reproducible techniques for sampling and sample treatment ...
Gregory D Tredwell, Bryn Edwards-Jones, David J Leak, Jacob G Bundy   +3 more
doaj   +1 more source

Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]

, 2003
The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
core  

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism [PDF]

Microbial Biotechnology, 2019
Summary Pichia pastoris KM 71H (Mut S ) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP ‐powered efflux pump which is expressed ...
Wan‐cang Liu, Fei Zhou, Di Xia, Joseph Shiloach   +3 more
openaire   +3 more sources

Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction

Microbial Cell Factories, 2007
BackgroundThe analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their interaction with the cultivation ...
David Resina, M. Bollók, N. K. Khatri, Francisco Valero, Peter Neubauer, P. Ferrer   +5 more
semanticscholar   +1 more source

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

Applied Microbiology and Biotechnology, 2021
Folding and processing of proteins in the endoplasmic reticulum (ER) are major impediments in the production and secretion of proteins from Pichia pastoris (Komagataella sp.).
Hana Raschmanová, A. Weninger, Z. Knejzlík, K. Melzoch, K. Kovar   +4 more
semanticscholar   +1 more source

Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Synthetic and Systems Biotechnology
Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P.
Shupeng Ruan, Yuxin Yang, Xinying Zhang, Guanjuan Luo, Ying Lin, Shuli Liang   +5 more
doaj   +1 more source

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy

Frontiers in Microbiology, 2020
Pichia pastoris (Komagataella phaffii) is a methylotrophic yeast that is widely used in industry as a host system for heterologous protein expression.
Chengbo Zhang, Yu Ma, Huabiao Miao, Xianghua Tang, Xianghua Tang, Xianghua Tang, Xianghua Tang, Bo Xu, Bo Xu, Bo Xu, Bo Xu, Qian Wu, Qian Wu, Qian Wu, Qian Wu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Yuelin Mu, Zunxi Huang, Zunxi Huang, Zunxi Huang, Zunxi Huang   +22 more
doaj   +1 more source