Results 51 to 60 of about 25,435,117 (220)

Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris

Bioengineering
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj   +1 more source

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

BMC Biotechnology, 2018
Background The methanol-regulated AOX1 promoter (P AOX1 ) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of P AOX1 restricts
Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong, Ching-Tsan Huang   +3 more
doaj   +1 more source

Putting System Co‐Design at the Center of Chemo‐Biohybrid CO2 Valorization From Lab to Industrial Scale

EcoEnergy, EarlyView.
Chemo‐biohybrid systems promise much, but single‐discipline research still limits progress. This perspective draws on thermocatalytic, electrocatalytic, and photocatalytic hybrid systems to highlight the need for system‐level co‐design and outline future directions.
Wenwen Yu, Hanjun Sun, Long Liu
wiley   +1 more source

Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism [PDF]

Microbial Biotechnology, 2019
Summary Pichia pastoris KM 71H (Mut S ) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP ‐powered efflux pump which is expressed ...
Wan‐cang Liu, Fei Zhou, Di Xia, Joseph Shiloach   +3 more
openaire   +3 more sources

The growth curves of phytase expressing P. pastoris with or without co-fermentation with Endo H-P expressing P. pastoris.

, 2015
(A).The growth curve of phytase- and Endo H-P-expressing P. pastoris during the vegetative growth phase. (B). The growth curve of phytase- and Endo H-P- expressing P. pastoris during the induction phase.
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source

Interactions between lactobacillus and yeast: A recent update

Food Biomacromolecules, EarlyView.
Abstract Lactobacillus and yeast are microorganisms widely distributed in nature. Their interactions significantly impact fermentation processes, human health, and medical care. However, there is a lack of a systematic summary of the characteristics of these interactions, and the mechanisms underlying these interactions remain unclear.
Yang Chen, Mengyuan Liu, Mengchen Li, Zhiyun Yue, Yang Mu, Chao Wang, Lusha Xia, Junjun Gao, Mingye Peng, Ran Wang, Mengzhou Zhou   +10 more
wiley   +1 more source

Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris [PDF]

Glycobiology, 2004
N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi.
Robert C, Davidson, Juergen H, Nett, Eduard, Renfer, Huijuan, Li, Terrance A, Stadheim, Benton J, Miller, Robert G, Miele, Stephen R, Hamilton, Byung-Kwon, Choi, Teresa I, Mitchell, Stefan, Wildt   +10 more
openaire   +2 more sources

Analysis of Carbohydrates and Glycoconjugates by Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2023–2024

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The use of MALDI mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this comprehensive review is the twelfth update of the original article published in 1999 and brings coverage of the literature to the end of 2024.
David J. Harvey
wiley   +1 more source

Co-fermentaion the phytase expressing P. pastoris and Endo H-P expressing P. pastoris with various initial ratio of cells innoculated in BMMY medium.

, 2015
M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 phytase expressed in P. pastoris; Lane 2–8 EndoH-P-expressing P. pastoris co-fermentated with phytase-expressing P. pastoris with inoculation ratio of 1:10, 1:25,
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source

Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Microbial Cell Factories, 2012
Background Lipase from Rhizopus chinensis is a versatile biocatalyst for various bioconversions and has been expressed at high-level in Pichia pastoris. However, the use of R.
Yu Xiao-Wei, Wang Rui, Zhang Meng, Xu Yan, Xiao Rong   +4 more
doaj   +1 more source