Results 31 to 40 of about 20,152,363 (248)
Journal of Virological Methods, 2002 The hepatitis B core antigen (HBcAg) was expressed in Escherichia coli and in Pichia pastoris. A hexahistidine tag was introduced at the C terminus of the E. coli expressed protein allowing its purification by Ni(2+)-chelate affinity chromatography. The P. pastoris expressed HBcAg was isolated following heat treatment.
Bénédicte Watelet +6 more
semanticscholar +3 more sources
Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale
Microbial Cell Factories, 2023 Background Methanol, synthesized from CO_2, is a potentially sustainable one-carbon (C1) resource for biomanufacturing. The use of methanol as a feedstock to produce single cell protein (SCP) has been investigated for decades as an alternative to ...
Jiao Meng +5 more
semanticscholar +1 more source
Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis
Advancement of science, 2023 Microbial synthesis of valuable hemoproteins has become a popular research topic, and Pichia pastoris is a versatile platform for the industrial production of recombinant proteins.
Fei Yu +6 more
semanticscholar +1 more source
Indonesian Journal of Chemistry, 2019 This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris.
Shabarni Gaffar +4 more
doaj +1 more source
Extracellular Production of the Taiwan-Native Norovirus P Domain Overexpressed in Pichia pastoris
Fermentation, 2023 Many efforts in norovirus vaccine development have focused on subunit or recombinant protein vaccines, such as subviral P particles formed by the protruding (P) domain of VP1. P particles are immunogenic and have a region with a human histo-blood group antigen binding site, an interaction critical for infecting the host. In the past, only intracellular
Man-Ling Chien +2 more
openaire +2 more sources
Microbial Cell Factories, 2010 Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system.
Chung Bevan KS +7 more
doaj +1 more source
Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. [PDF]
PLoS ONE, 2013 Pichia pastoris is commonly used for the production of recombinant proteins due to its preferential secretion of recombinant proteins, resulting in lower production costs and increased yields of target proteins.
Jian Tian +8 more
doaj +1 more source
Stereoselective Biotransformation: Transfer of Learning to Advance Drug Metabolism and Biocatalysis
Angewandte Chemie, EarlyView.Understanding stereoselective biotransformations has implications for predicting drug disposition and response and may also inspire novel biocatalytic and biomimetic strategies to address challenges in metabolite and API synthesis. ABSTRACT Chirality is an important determinant of drug action, as enantiomers can exhibit markedly different ...
Grace A. Okunlola, Godwin A. Aleku
wiley +2 more sources
BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2022 Significance Methanol-based biomanufacturing is considered a promising way to achieve carbon neutrality. However, efficient methanol biotransformation toward chemical production is challenging.
Peng Cai +6 more
semanticscholar +1 more source