Results 31 to 40 of about 20,634,842 (205)

Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale

Microbial Cell Factories, 2023
Background Methanol, synthesized from CO_2, is a potentially sustainable one-carbon (C1) resource for biomanufacturing. The use of methanol as a feedstock to produce single cell protein (SCP) has been investigated for decades as an alternative to ...
Jiao Meng, Shufan Liu, Le Gao, Kai Hong, Shuguang Liu, Xin Wu   +5 more
semanticscholar   +1 more source

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Microbial Cell Factories, 2010
Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system.
Chung Bevan KS, Selvarasu Suresh, Andrea Camattari, Ryu Jimyoung, Lee Hyeokweon, Ahn Jungoh, Lee Hongweon, Lee Dong-Yup   +7 more
doaj   +1 more source

Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis

Advancement of science, 2023
Microbial synthesis of valuable hemoproteins has become a popular research topic, and Pichia pastoris is a versatile platform for the industrial production of recombinant proteins.
Fei Yu, Xinrui Zhao, Jingwen Zhou, Wei Lu, Jianghua Li, Jian Chen, G. Du   +6 more
semanticscholar   +1 more source

Combination of Genetic Manipulation Improved Saccharomycopsis fibuligera α-Amylase Secretion by Pichia pastoris

Indonesian Journal of Chemistry, 2019
This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris.
Shabarni Gaffar, Dessy Natalia, Toto Subroto, Oo Suprijana, Soetijoso Soemitro   +4 more
doaj   +1 more source

Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

BMC Biotechnology, 2008
Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu, Tao Xingyi, Ren Ren, Gao Bei, Jiang Zhengbing, Wei Dongzhi   +5 more
doaj   +1 more source

Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. [PDF]

PLoS ONE, 2013
Pichia pastoris is commonly used for the production of recombinant proteins due to its preferential secretion of recombinant proteins, resulting in lower production costs and increased yields of target proteins.
Jian Tian, Yuhong Zhang, Bo Liu, Dongyang Zuo, Tao Jiang, Jun Guo, Wei Zhang, Ningfeng Wu, Yunliu Fan   +8 more
doaj   +1 more source

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance Methanol-based biomanufacturing is considered a promising way to achieve carbon neutrality. However, efficient methanol biotransformation toward chemical production is challenging.
Peng Cai, Xiaoyan Wu, Jun Deng, Linhui Gao, Yiwei Shen, Lun Yao, Yongjin J. Zhou   +6 more
semanticscholar   +1 more source

Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Biotechnology for Biofuels, 2019
Background Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient.
Yide Huang, Yafei Zhang, Suhuan Li, Ting Lin, Jingwen Wu, Yao Lin   +5 more
doaj   +1 more source

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Microbial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors

AMB Express, 2021
The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals.
Barbara G. Paes, Andrei Stecca Steindorff, Eduardo F. Formighieri, Ildinete Silva Pereira, João Ricardo M. Almeida   +4 more
doaj   +1 more source