Results 31 to 40 of about 25,435,117 (220)

Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors

AMB Express, 2021
The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals.
Barbara G. Paes, Andrei Stecca Steindorff, Eduardo F. Formighieri, Ildinete Silva Pereira, João Ricardo M. Almeida   +4 more
doaj   +1 more source

Extracellular Production of the Taiwan-Native Norovirus P Domain Overexpressed in Pichia pastoris

Fermentation, 2023
Many efforts in norovirus vaccine development have focused on subunit or recombinant protein vaccines, such as subviral P particles formed by the protruding (P) domain of VP1. P particles are immunogenic and have a region with a human histo-blood group antigen binding site, an interaction critical for infecting the host. In the past, only intracellular
Chien, Man-Ling, Yu, Chun-Fu, Huang, Ching-Tsan   +2 more
openaire   +2 more sources

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Microbial Cell Factories, 2004
Background Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty.
Porro Danilo, Maurer Michael, Valli Minoska, Gasser Brigitte, Branduardi Paola, Sauer Michael, Mattanovich Diethard   +6 more
doaj   +1 more source

Screening and evaluation of the strong endogenous promoters in Pichia pastoris

Microbial Cell Factories, 2021
Background Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds.
Weiwang Dou, Quanchao Zhu, Meihua Zhang, Zuyuan Jia, Wenjun Guan   +4 more
doaj   +1 more source

Results of screening of culture supernatants of P. pastoris X-33_pPICZα/XynT6.

, 2022
Results of screening of culture supernatants of P. pastoris X-33_pPICZα/XynT6.
Assel Kiribayeva (12257166), Birzhan Mukanov (12257169), Zhiger Akishev (12257175), Bekbolat Khassenov (5635241), Yerlan Ramankulov (5635238), Dmitriy Silayev (12257172)   +5 more
core   +1 more source

Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs

Bioresources and Bioprocessing, 2017
Background Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P.
Rui Ye, Mingzhi Huang, Hongzhong Lu, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang, Siliang Zhang   +7 more
doaj   +1 more source

List of P. pastoris strains.

, 2014
List of P. pastoris strains.
Oded Lewinson (638433), Tsvia Gildor (469231), Sarah J. Kauffman (638432), Ziva Weissman (638431), Elena Vigonsky (638430), Galit Kuznets (638429), Daniel Kornitzer (469233), Daniela Lalli (224677), Paola Turano (224679), Jeffrey Becker (5660842)   +9 more
core   +1 more source

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Microbial Cell Factories, 2012
Background Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P ...
Chen Ming-Tang, Lin Song, Shandil Ishaan, Andrews Dewan, Stadheim Terrance A, Choi Byung-Kwon   +5 more
doaj   +1 more source

Tailored Covalent Organic Frameworks Enable the Encapsulation of Living Cells to Produce Rare Ginsenoside

Advanced Science, EarlyView.
This work reports the in situ fabrication of Escherichia coli@covalent organic frameworks (E. coli@COFs) in phosphate‐buffered saline. The COFs form an exoskeleton on E. coli, protecting intracellular β‐glucosidase (Ssbgl) while enhancing mass transfer.
Fengmei Liu, Shan Zhang, Shanrong Wei, Ziye Xing, Hongming He, Xiao Liang, Quanshun Li   +6 more
wiley   +1 more source

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source