Results 31 to 40 of about 47,430 (223)
Microbial Cell Factories, 2010 Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system.
Chung Bevan KS +7 more
doaj +1 more source
Indonesian Journal of Chemistry, 2019 This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris.
Shabarni Gaffar +4 more
doaj +1 more source
Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. [PDF]
PLoS ONE, 2013 Pichia pastoris is commonly used for the production of recombinant proteins due to its preferential secretion of recombinant proteins, resulting in lower production costs and increased yields of target proteins.
Jian Tian +8 more
doaj +1 more source
Novel homologous lactate transporter improves l‑lactic acid production from glycerol in recombinant strains of Pichia pastoris [PDF]
, 2016 Background: Crude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude ...
Carvalho, Lucas Silva +10 more
core +1 more source
BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
doaj +1 more source
AMB Express, 2021 The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals.
Barbara G. Paes +4 more
doaj +1 more source
Pichia pastoris Fep1 is a [2Fe-2S] protein with a Zn finger that displays an unusual oxygen-dependent role in cluster binding [PDF]
, 2016 Fep1, the iron-responsive GATA factor from the methylotrophic yeast Pichia pastoris, has been characterised both in vivo and in vitro. This protein has two Cys(2)-Cys(2) type zinc fingers and a set of four conserved cysteines arranged in a Cys-X-5-Cys-X ...
Battistoni, Andrea +8 more
core +4 more sources
Stereoselective Biotransformation: Transfer of Learning to Advance Drug Metabolism and Biocatalysis
Angewandte Chemie, EarlyView.Understanding stereoselective biotransformations has implications for predicting drug disposition and response and may also inspire novel biocatalytic and biomimetic strategies to address challenges in metabolite and API synthesis. ABSTRACT Chirality is an important determinant of drug action, as enantiomers can exhibit markedly different ...
Grace A. Okunlola, Godwin A. Aleku
wiley +2 more sources
Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris
Biotechnology for Biofuels, 2019 Background Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient.
Yide Huang +5 more
doaj +1 more source
The autophagic degradation of cytosolic pools of peroxisomal proteins by a new selective pathway. [PDF]
, 2019 Damaged or redundant peroxisomes and their luminal cargoes are removed by pexophagy, a selective autophagy pathway. In yeasts, pexophagy depends mostly on the pexophagy receptors, such as Atg30 for Pichia pastoris and Atg36 for Saccharomyces cerevisiae ...
Farré, Jean-Claude +8 more
core +2 more sources