Results 11 to 20 of about 20,152,363 (248)
Compact multi-enzyme pathways in P. pastoris.
Chemical Communications, 2015 We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production ...
M. Geier +3 more
semanticscholar +4 more sources
Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris
, 2022 Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E.
Pauline, Lenkeit Gesser +6 more
core +3 more sources
Multistep metabolic engineering of Pichia pastoris for biosynthesis of N‑acetylneuraminic acid [PDF]
Applied Microbiology and BiotechnologyFree or protein-conjugated N-Acetylneuraminic acid (Neu5Ac) is widely distributed in organisms and possesses diverse biological functions, and for example lactoferrin with Neu5Ac-mediated sialylation exhibits significantly enhanced functional activity ...
Xiaomin Zhao +7 more
doaj +2 more sources
Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris [PDF]
Synthetic and Systems BiotechnologySynthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations in production
Bo Sun +10 more
doaj +2 more sources
Low-cost media statistical design for laccase rPOXA 1B production in P. pastoris. [PDF]
Heliyon, 2020 Laccases (E.C. 1.10.3.2) are multicopper oxidases of great importance in the industry due to their non-specificity and high oxidative potential. Laccases are useful to bleach synthetic dyes, oxidize phenolic compounds and degrade pesticides, among others. Hence, the objective of this work was to optimize low cost culture media for recombinant (rPOXA 1B)
Ardila-Leal LD +6 more
europepmc +5 more sources
Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. [PDF]
Journal of Histochemistry & Cytochemistry, 1996 We exploited the light-activated fluorescent properties of the green fluorescent protein (GFP) of the jellyfish Aequorea victoria for studies on the peroxisomal sorting of polypeptides. GFP and GFP-SKL (containing a C-terminal, tripeptide peroxisomal targeting signal, SKL) were expressed from a methanol-inducible, alcohol oxidase (AOX1) promoter in the
E. Monosov +4 more
semanticscholar +3 more sources
Media improvement for 10 L bioreactor production of rPOXA 1B laccase by P. pastoris. [PDF]
3 Biotech, 2019 In this work, we statistically improved culture media for rPOXA 1B laccase production, expressed in Pichia pastoris containing pGAPZαA-LaccPost-Stop construct and assayed at 10 L bioreactor production scale (6 L effective work volume). The concentrated enzyme was evaluated for temperature and pH stability and kinetic parameter, characterized by ...
Ardila-Leal LD +11 more
europepmc +4 more sources
Synthetic and Systems Biotechnology, 2021 The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj +1 more source
Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]
Brazilian Archives of Biology and Technology, 2020 Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos +12 more
doaj +1 more source
AMB Express, 2020 In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao +6 more
doaj +1 more source