Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris
, 2022 Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli.
Anja, Håkansson +6 more
core +5 more sources
The impact of oxygen on the transcriptome of recombinant
BMC Genomics, 2011 Background Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins.
Porro Danilo +8 more
doaj +8 more sources
Ecophysiological approaches to enhance production of the anti-cancer drug taxol by paraconiothyrium variabile and epiccocum nigrum and lysozyme by pichia pastoris [PDF]
, 2013 This study has investigated the interaction of ecophysiological factors such as water activity (aw), temperature, pH and solute types on (a) the production of the anti-cancer drug taxol by the endophytic fungi Parachoniothyrium variabile and Epcoccum ...
Somjaipeng, Supunnika
core +7 more sources
Low-cost media statistical design for laccase rPOXA 1B production in P. pastoris. [PDF]
Heliyon, 2020 Laccases (E.C. 1.10.3.2) are multicopper oxidases of great importance in the industry due to their non-specificity and high oxidative potential. Laccases are useful to bleach synthetic dyes, oxidize phenolic compounds and degrade pesticides, among others. Hence, the objective of this work was to optimize low cost culture media for recombinant (rPOXA 1B)
Ardila-Leal LD +6 more
europepmc +5 more sources
Media improvement for 10 L bioreactor production of rPOXA 1B laccase by P. pastoris. [PDF]
3 Biotech, 2019 In this work, we statistically improved culture media for rPOXA 1B laccase production, expressed in Pichia pastoris containing pGAPZαA-LaccPost-Stop construct and assayed at 10 L bioreactor production scale (6 L effective work volume). The concentrated enzyme was evaluated for temperature and pH stability and kinetic parameter, characterized by ...
Ardila-Leal LD +11 more
europepmc +4 more sources
Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris [PDF]
, 2013 Background: Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in ...
Harvey, Linda M. +4 more
core +4 more sources
A Cytoplasm to Vacuole Targeting Pathway inP. pastoris [PDF]
Autophagy, 2007 The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape1) from the cytosol to the vacuole, bypassing the normal secretory route. The Cvt pathway, although well-studied, was known only in S. cerevisiae. We demonstrate its existence in the methylotrophic yeast, Pichia pastoris, where it also delivers P.
Farré, Jean-Claude +2 more
openaire +3 more sources
Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. [PDF]
Journal of Histochemistry & Cytochemistry, 1996 We exploited the light-activated fluorescent properties of the green fluorescent protein (GFP) of the jellyfish Aequorea victoria for studies on the peroxisomal sorting of polypeptides. GFP and GFP-SKL (containing a C-terminal, tripeptide peroxisomal targeting signal, SKL) were expressed from a methanol-inducible, alcohol oxidase (AOX1) promoter in the
E Z, Monosov +4 more
openaire +2 more sources
Compact multi-enzyme pathways in P. pastoris
Chemical Communications, 2015 2A peptides enabling the coordinate expression of nine genes in P. pastoris represent a valuable tool for pathway construction and engineering.
Geier +4 more
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Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris [PDF]
Applied Microbiology and Biotechnology, 2018 The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host's immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed through intermolecular contact in the
Yu-Ling Chen +2 more
openaire +2 more sources