Journal of Cellular Physiology, 2020 One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts,
S A Rahim Rezaee +2 more
exaly +2 more sources
Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Journal of Fungi, 2023 Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha +4 more
doaj +2 more sources
Media improvement for 10 L bioreactor production of rPOXA 1B laccase by P. pastoris. [PDF]
3 Biotech, 2019 In this work, we statistically improved culture media for rPOXA 1B laccase production, expressed in Pichia pastoris containing pGAPZαA-LaccPost-Stop construct and assayed at 10 L bioreactor production scale (6 L effective work volume). The concentrated enzyme was evaluated for temperature and pH stability and kinetic parameter, characterized by ...
Ardila-Leal LD +11 more
europepmc +4 more sources
Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris. [PDF]
Glycobiology, 2004 N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi.
Robert C. Davidson +10 more
semanticscholar +3 more sources
Production of isotope-labeled nanobody KN035 in Pichia pastoris yields native-like conformation and PD-L1 binding activity comparable to KN035 expressed in mammalian cells [PDF]
Magnetic Resonance LettersStable isotope-labeled proteins are indispensable for NMR-based structural studies. E. coli is a cost-effective and high-yield expression host, but it often fails to support proper folding and post-translational modifications required for functional ...
Piaoran Ren +2 more
doaj +2 more sources
Methanol-based biosynthesis of p-coumaric acid by engineered Pichia pastoris. [PDF]
Bioresour BioprocessAbstract p -Coumaric acid (p-CA) is a key aromatic precursor for the biosynthesis of flavonoids, stilbenoids, and other high-value phenylpropanoids. While microbial production of p-CA typically relies on sugar-based substrates, methanol offers a sustainable and cost-effective alternative ...
Chen M +7 more
europepmc +2 more sources
Synthetic and Systems Biotechnology, 2023 The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter (pAOX1). However, it is difficult to use P.
Xiang Wang +6 more
doaj +2 more sources
Multistep metabolic engineering of Pichia pastoris for biosynthesis of N‑acetylneuraminic acid [PDF]
Applied Microbiology and BiotechnologyFree or protein-conjugated N-Acetylneuraminic acid (Neu5Ac) is widely distributed in organisms and possesses diverse biological functions, and for example lactoferrin with Neu5Ac-mediated sialylation exhibits significantly enhanced functional activity ...
Xiaomin Zhao +7 more
doaj +2 more sources
Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris [PDF]
Synthetic and Systems BiotechnologySynthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations in production
Bo Sun +10 more
doaj +2 more sources
Recombinant cryptogein production by P. pastoris
New Biotechnology, 2012 Michal Obořil +4 more
semanticscholar +2 more sources