Results 21 to 30 of about 20,634,842 (205)

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Synthetic and Systems Biotechnology, 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj   +1 more source

Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]

Brazilian Archives of Biology and Technology, 2020
Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos, Michele Pepe Cerqueira, Giana Carla Gaboardi, Carolina Georg Magalhães, Rafael Amaral Donassolo, Rafael Rodrigues Rodrigues, Emili Griep, Marcos Roberto Ferreira, Guita Rubinsky Elefant, Luciana Farias da Costa Avila, Carlos James Scaini, Ângela Nunes Moreira, Fabricio Rochedo Conceição   +12 more
doaj   +1 more source

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
doaj   +1 more source

Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

Frontiers in Bioengineering and Biotechnology, 2021
The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales.
Gita Naseri, Kevin Prause, Housam Haj Hamdo, Christoph Arenz   +3 more
doaj   +1 more source

Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

, 2022
Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli.
Anja, Håkansson, Therese, Dalén, Josefine, Gröblacher, William, Göransson, Jonathan, Jaksties, Nathalie, Ortstad, Pauline, Lenkeit Gesser   +6 more
openaire   +2 more sources

Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris [PDF]

Applied Microbiology and Biotechnology, 2018
The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host's immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed through intermolecular contact in the
Yu-Ling Chen, Pey-Jium Chang, Ching-Tsan Huang   +2 more
openaire   +2 more sources

Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris

Microbial Cell Factories, 2018
Background Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal.
Yide Huang, Yanyu Long, Suhuan Li, Ting Lin, Jingwen Wu, Yafei Zhang, Yao Lin   +6 more
doaj   +1 more source

Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Microbial Cell Factories, 2011
Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi, Ito Keisuke, Tsujimoto Hirokazu, Shimamura Tatsuro, Shiroishi Mitsunori, Yurugi-Kobayashi Takami, Uemura Tomoko, Asada Hidetsugu, Nakane Takanori, Nomura Norimichi, Murata Takeshi, Haga Tatsuya, Iwata So, Kobayashi Takuya   +13 more
doaj   +1 more source

Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

BMC Genomics, 2012
Background The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and ...
Liang Shuli, Wang Bin, Pan Li, Ye Yanrui, He Minghui, Han Shuangyan, Zheng Suiping, Wang Xiaoning, Lin Ying   +8 more
doaj   +1 more source

Surface display and bioactivity of Bombyx mori acetylcholinesterase on Pichia pastoris. [PDF]

PLoS ONE, 2013
A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied.
Jie-Xian Dong, Xi Xie, Yong-Sheng He, Ross C Beier, Yuan-Ming Sun, Zhen-Lin Xu, Wei-Jian Wu, Yu-Dong Shen, Zhi-Li Xiao, Li-Na Lai, Hong Wang, Jin-Yi Yang   +11 more
doaj   +1 more source