Results 21 to 30 of about 20,152,363 (248)
Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris. [PDF]
Glycobiology, 2004 N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi.
Robert C. Davidson +10 more
semanticscholar +3 more sources
Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
Frontiers in Bioengineering and Biotechnology, 2021 The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales.
Gita Naseri +3 more
doaj +1 more source
Design and construction of light-regulated gene transcription and protein translation systems in yeast P. Pastoris. [PDF]
J Adv ResP. pastoris is a common host for effective biosynthesis of heterologous proteins as well as small molecules. Accurate regulation of gene transcription and protein synthesis is necessary to coordinate synthetic gene circuits and optimize cellular energy distribution.
Zhang S +7 more
europepmc +4 more sources
Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris [PDF]
Applied Microbiology and Biotechnology, 2018 The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host's immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed through intermolecular contact in the
Yu-Ling Chen +2 more
openaire +2 more sources
Microbial Cell Factories, 2018 Background Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal.
Yide Huang +6 more
doaj +1 more source
Microbial Cell Factories, 2011 Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi +13 more
doaj +1 more source
Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
core +1 more source
BMC Genomics, 2012 Background The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and ...
Liang Shuli +8 more
doaj +1 more source
Surface display and bioactivity of Bombyx mori acetylcholinesterase on Pichia pastoris. [PDF]
PLoS ONE, 2013 A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied.
Jie-Xian Dong +11 more
doaj +1 more source
Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris [PDF]
, 2013 Background: Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in ...
Harvey, Linda M. +11 more
core +1 more source