Microbial Cell Factories, 2011 Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi +13 more
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Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1 [PDF]
, 2018 The Starmerella bombicola lactone esterase (SBLE) is a novel enzyme that, in vivo, catalyzes the intramolecular esterification (lactonization) of acidic sophorolipids in an aqueous environment.
Ciesielska, Katarzyna +8 more
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BMC Genomics, 2012 Background The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and ...
Liang Shuli +8 more
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Surface display and bioactivity of Bombyx mori acetylcholinesterase on Pichia pastoris. [PDF]
PLoS ONE, 2013 A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied.
Jie-Xian Dong +11 more
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Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Journal of Fungi, 2023 Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha +4 more
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Production of a soluble and functional recombinant apolipoproteinD in the Pichia pastoris expression system [PDF]
, 2016 ApolipoproteinD (ApoD) is a human glycoprotein from the lipocalin family. ApoD contains a conserved central motif of an 8-stranded antiparallel β-sheet, which forms a beta-barrel that can be used for transport and storage of diverse hydrophobic ligands ...
Armanmehr, S. +2 more
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Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone [PDF]
, 2013 The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of
Altschul +30 more
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Species-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramides [PDF]
, 2019 AFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and is a very potent inhibitor of fungal growth that does not affect the viability of bacteria, plant, or mammalian cells.
Baumann, Birgit +8 more
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Extracellular Production of the Taiwan-Native Norovirus P Domain Overexpressed in Pichia pastoris
Fermentation, 2023 Many efforts in norovirus vaccine development have focused on subunit or recombinant protein vaccines, such as subviral P particles formed by the protruding (P) domain of VP1. P particles are immunogenic and have a region with a human histo-blood group antigen binding site, an interaction critical for infecting the host. In the past, only intracellular
Man-Ling Chien +2 more
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Applied Microbiology and Biotechnology / The bud tip is the cellular hot spot of protein secretion in yeasts [PDF]
, 2016 in deutscher Sprache nicht verf\ufcgbarYeasts are valuable hosts for recombinant protein production. Among them, Pichia pastoris is frequently used for production of secreted proteins, and much effort was made to improve the secretion efficiency of this ...
Gasser, Brigitte +2 more
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