Results 21 to 30 of about 23,995,070 (219)
Microbial Cell Factories, 2011 Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi +13 more
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BMC Genomics, 2012 Background The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and ...
Liang Shuli +8 more
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Surface display and bioactivity of Bombyx mori acetylcholinesterase on Pichia pastoris. [PDF]
PLoS ONE, 2013 A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied.
Jie-Xian Dong +11 more
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Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Journal of Fungi, 2023 Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha +4 more
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Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
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Microbial Cell Factories, 2010 Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system.
Chung Bevan KS +7 more
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BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
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Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. [PDF]
PLoS ONE, 2013 Pichia pastoris is commonly used for the production of recombinant proteins due to its preferential secretion of recombinant proteins, resulting in lower production costs and increased yields of target proteins.
Jian Tian +8 more
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Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]
, 2003 The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
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Indonesian Journal of Chemistry, 2019 This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris.
Shabarni Gaffar +4 more
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