Results 21 to 30 of about 25,435,117 (220)

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Journal of Fungi, 2023
Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha, Dan Liu, Juan Ren, Zhijun Liu, Xia Wu   +4 more
doaj   +1 more source

Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]

, 2005
CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J., Blower, Philip J., Peter J. Nicholls, Trivett, Amanda J., Blower, P J, Emberson, L M, Philip J. Blower, Nicholls, P J, Amanda J. Trivett, Louise M. Emberson, Emberson, Louise M., Trivett, A J   +11 more
core   +1 more source

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Microbial Cell Factories, 2010
Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system.
Chung Bevan KS, Selvarasu Suresh, Andrea Camattari, Ryu Jimyoung, Lee Hyeokweon, Ahn Jungoh, Lee Hongweon, Lee Dong-Yup   +7 more
doaj   +1 more source

Combination of Genetic Manipulation Improved Saccharomycopsis fibuligera α-Amylase Secretion by Pichia pastoris

Indonesian Journal of Chemistry, 2019
This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris.
Shabarni Gaffar, Dessy Natalia, Toto Subroto, Oo Suprijana, Soetijoso Soemitro   +4 more
doaj   +1 more source

Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris [PDF]

, 2011
Includes bibliographical references (leaves 98-103).The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics.
Bhardwaj, Vinayak
core   +1 more source

Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

BMC Biotechnology, 2008
Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu, Tao Xingyi, Ren Ren, Gao Bei, Jiang Zhengbing, Wei Dongzhi   +5 more
doaj   +1 more source

Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. [PDF]

PLoS ONE, 2013
Pichia pastoris is commonly used for the production of recombinant proteins due to its preferential secretion of recombinant proteins, resulting in lower production costs and increased yields of target proteins.
Jian Tian, Yuhong Zhang, Bo Liu, Dongyang Zuo, Tao Jiang, Jun Guo, Wei Zhang, Ningfeng Wu, Yunliu Fan   +8 more
doaj   +1 more source

Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Biotechnology for Biofuels, 2019
Background Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient.
Yide Huang, Yafei Zhang, Suhuan Li, Ting Lin, Jingwen Wu, Yao Lin   +5 more
doaj   +1 more source

Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]

, 2003
The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
core   +2 more sources

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Microbial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source