Results 1 to 10 of about 25,435,117 (220)

Multistep metabolic engineering of Pichia pastoris for biosynthesis of N‑acetylneuraminic acid [PDF]

Applied Microbiology and Biotechnology
Free or protein-conjugated N-Acetylneuraminic acid (Neu5Ac) is widely distributed in organisms and possesses diverse biological functions, and for example lactoferrin with Neu5Ac-mediated sialylation exhibits significantly enhanced functional activity ...
Xiaomin Zhao, Qian Li, Huiying Luo, Yuan Wang, Xinxin Xu, Wei Zhang, Jie Zhang, Bo Liu   +7 more
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Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Synthetic and Systems Biotechnology, 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
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Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]

Brazilian Archives of Biology and Technology, 2020
Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos, Michele Pepe Cerqueira, Giana Carla Gaboardi, Carolina Georg Magalhães, Rafael Amaral Donassolo, Rafael Rodrigues Rodrigues, Emili Griep, Marcos Roberto Ferreira, Guita Rubinsky Elefant, Luciana Farias da Costa Avila, Carlos James Scaini, Ângela Nunes Moreira, Fabricio Rochedo Conceição   +12 more
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Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
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Second generation Pichia pastoris strain and bioprocess designs

Biotechnology for Biofuels and Bioproducts, 2022
Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün, Kübra Laçın, Buse Çaloğlu, Barış Binay   +3 more
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Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

Frontiers in Bioengineering and Biotechnology, 2021
The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales.
Gita Naseri, Kevin Prause, Housam Haj Hamdo, Christoph Arenz   +3 more
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Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris

Microbial Cell Factories, 2018
Background Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal.
Yide Huang, Yanyu Long, Suhuan Li, Ting Lin, Jingwen Wu, Yafei Zhang, Yao Lin   +6 more
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Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Microbial Cell Factories, 2011
Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi, Ito Keisuke, Tsujimoto Hirokazu, Shimamura Tatsuro, Shiroishi Mitsunori, Yurugi-Kobayashi Takami, Uemura Tomoko, Asada Hidetsugu, Nakane Takanori, Nomura Norimichi, Murata Takeshi, Haga Tatsuya, Iwata So, Kobayashi Takuya   +13 more
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Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

BMC Genomics, 2012
Background The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and ...
Liang Shuli, Wang Bin, Pan Li, Ye Yanrui, He Minghui, Han Shuangyan, Zheng Suiping, Wang Xiaoning, Lin Ying   +8 more
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Surface display and bioactivity of Bombyx mori acetylcholinesterase on Pichia pastoris. [PDF]

PLoS ONE, 2013
A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied.
Jie-Xian Dong, Xi Xie, Yong-Sheng He, Ross C Beier, Yuan-Ming Sun, Zhen-Lin Xu, Wei-Jian Wu, Yu-Dong Shen, Zhi-Li Xiao, Li-Na Lai, Hong Wang, Jin-Yi Yang   +11 more
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