Results 1 to 10 of about 47,363 (163)
Multistep metabolic engineering of Pichia pastoris for biosynthesis of N‑acetylneuraminic acid [PDF]
Applied Microbiology and BiotechnologyFree or protein-conjugated N-Acetylneuraminic acid (Neu5Ac) is widely distributed in organisms and possesses diverse biological functions, and for example lactoferrin with Neu5Ac-mediated sialylation exhibits significantly enhanced functional activity ...
Xiaomin Zhao +7 more
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Heterologous overexpression of
Microbial Cell Factories, 2011 Background FAD dependent glucose dehydrogenase (GDH) currently raises enormous interest in the field of glucose biosensors. Due to its superior properties such as high turnover rate, substrate specificity and oxygen independence, GDH makes its way into ...
Sygmund Christoph +7 more
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Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris [PDF]
Synthetic and Systems BiotechnologySynthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations in production
Bo Sun +10 more
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Synthetic and Systems Biotechnology, 2021 The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
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Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]
Brazilian Archives of Biology and Technology, 2020 Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos +12 more
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AMB Express, 2020 In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao +6 more
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Second generation Pichia pastoris strain and bioprocess designs
Biotechnology for Biofuels and Bioproducts, 2022 Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün +3 more
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Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
Frontiers in Bioengineering and Biotechnology, 2021 The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales.
Gita Naseri +3 more
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Conformational Transitions Accompanying Oligomerization of Yeast Alcohol Oxidase, a Peroxisomal Flavoenzyme [PDF]
, 1999 Alcohol oxidase (AO) is a homo-octameric flavoenzyme which catalyzes methanol oxidation in methylotrophic yeasts. AO protein is synthesized in the cytosol and subsequently sorted to peroxisomes where the active enzyme is formed.
Boteva, R +6 more
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Microbial Cell Factories, 2018 Background Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal.
Yide Huang +6 more
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