Results 1 to 10 of about 23,995,070 (219)

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production. [PDF]

Adv Sci (Weinh)
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Du Y, Pi C, Hong K, Gao L, Wu X.
europepmc   +2 more sources

Real-Time Biomass Estimation in High-Density Yeast Fermentations Using Soft Sensor Modeling. [PDF]

Biotechnol Bioeng
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Del Hierro AG, Checa-Barrera JL, Moreno-Cid JA, Casey E.   +3 more
europepmc   +2 more sources

Production of isotope-labeled nanobody KN035 in Pichia pastoris yields native-like conformation and PD-L1 binding activity comparable to KN035 expressed in mammalian cells [PDF]

Magnetic Resonance Letters
Stable isotope-labeled proteins are indispensable for NMR-based structural studies. E. coli is a cost-effective and high-yield expression host, but it often fails to support proper folding and post-translational modifications required for functional ...
Piaoran Ren, Mengzhen Jiang, Shenlin Wang   +2 more
doaj   +2 more sources

Multistep metabolic engineering of Pichia pastoris for biosynthesis of N‑acetylneuraminic acid [PDF]

Applied Microbiology and Biotechnology
Free or protein-conjugated N-Acetylneuraminic acid (Neu5Ac) is widely distributed in organisms and possesses diverse biological functions, and for example lactoferrin with Neu5Ac-mediated sialylation exhibits significantly enhanced functional activity ...
Xiaomin Zhao, Qian Li, Huiying Luo, Yuan Wang, Xinxin Xu, Wei Zhang, Jie Zhang, Bo Liu   +7 more
doaj   +2 more sources

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Synthetic and Systems Biotechnology, 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj   +1 more source

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
doaj   +1 more source

Second generation Pichia pastoris strain and bioprocess designs

Biotechnology for Biofuels and Bioproducts, 2022
Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün, Kübra Laçın, Buse Çaloğlu, Barış Binay   +3 more
doaj   +1 more source

Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]

Brazilian Archives of Biology and Technology, 2020
Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos, Michele Pepe Cerqueira, Giana Carla Gaboardi, Carolina Georg Magalhães, Rafael Amaral Donassolo, Rafael Rodrigues Rodrigues, Emili Griep, Marcos Roberto Ferreira, Guita Rubinsky Elefant, Luciana Farias da Costa Avila, Carlos James Scaini, Ângela Nunes Moreira, Fabricio Rochedo Conceição   +12 more
doaj   +1 more source

Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

Frontiers in Bioengineering and Biotechnology, 2021
The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales.
Gita Naseri, Kevin Prause, Housam Haj Hamdo, Christoph Arenz   +3 more
doaj   +1 more source

Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris

Microbial Cell Factories, 2018
Background Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal.
Yide Huang, Yanyu Long, Suhuan Li, Ting Lin, Jingwen Wu, Yafei Zhang, Yao Lin   +6 more
doaj   +1 more source