The impact of oxygen on the transcriptome of recombinant
BMC Genomics, 2011 Background Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins.
Porro Danilo +8 more
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Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris. [PDF]
Microb Cell Fact, 2021 Background Protein synthesis is one of the extremely important anabolic pathways in the yeast expression system Pichia pastoris . Codon optimization is a commonly adopted strategy for improved protein expression, although unexpected failures did appear ...
Xu Y +6 more
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A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System. [PDF]
Molecules, 2019 Cell surface display systems for immobilization of peptides and proteins on the surface of cells have various applications, such as vaccine generation, protein engineering, bio-conversion and bio-adsorption. Though plenty of methods have been established
Li S, Qiao J, Lin S, Liu Y, Ma L.
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A Cytoplasm to Vacuole Targeting Pathway in P. pastoris [PDF]
Autophagy, 2007 The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape1) from the cytosol to the vacuole, bypassing the normal secretory route. The Cvt pathway, although well-studied, was known only in S. cerevisiae. We demonstrate its existence in the methylotrophic yeast, Pichia pastoris, where it also delivers P.
Jean-Claude Farré +2 more
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Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica. [PDF]
Front Microbiol, 2017 To study hydrolysis of aromatic and aliphatic polyesters cutinase 1 from Thermobifida cellulosilytica (Thc_Cut1) was expressed in P. pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out ...
Gamerith C +9 more
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Fine-tuning the P. pastoris iMT1026 genome-scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources. [PDF]
Microb Biotechnol, 2018 The methylotrophic yeast Pichia pastoris (Komagataella spp.) is widely used as cell factory for recombinant protein production. In the past recent years, important breakthroughs in the systems‐level quantitative analysis of its physiology have been ...
Tomàs-Gamisans M, Ferrer P, Albiol J.
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Compact multi-enzyme pathways in P. pastoris.
Chemical Communications, 2015 We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production ...
M. Geier +3 more
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Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. [PDF]
Journal of Histochemistry & Cytochemistry, 1996 We exploited the light-activated fluorescent properties of the green fluorescent protein (GFP) of the jellyfish Aequorea victoria for studies on the peroxisomal sorting of polypeptides. GFP and GFP-SKL (containing a C-terminal, tripeptide peroxisomal targeting signal, SKL) were expressed from a methanol-inducible, alcohol oxidase (AOX1) promoter in the
E. Monosov +4 more
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Low-cost media statistical design for laccase rPOXA 1B production in P. pastoris. [PDF]
Heliyon, 2020 Laccases (E.C. 1.10.3.2) are multicopper oxidases of great importance in the industry due to their non-specificity and high oxidative potential. Laccases are useful to bleach synthetic dyes, oxidize phenolic compounds and degrade pesticides, among others. Hence, the objective of this work was to optimize low cost culture media for recombinant (rPOXA 1B)
Ardila-Leal LD +6 more
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Second generation Pichia pastoris strain and bioprocess designs
Biotechnology for Biofuels and Bioproducts, 2022 Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün +3 more
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