A cytoplasm to vacuole targeting pathway in P-pastoris [PDF]
Autophagy, 2007 The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape 1) from the cytosol to the vacuole, bypassing the normal secretary route. The Cvt pathway, although well-studied, was known only in S. cerevisiae.
Farré, Jean-Claude +2 more
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Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica. [PDF]
Front Microbiol, 2017 To study hydrolysis of aromatic and aliphatic polyesters cutinase 1 from Thermobifida cellulosilytica (Thc_Cut1) was expressed in P. pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out ...
Gamerith C +9 more
europepmc +9 more sources
The impact of oxygen on the transcriptome of recombinant
BMC Genomics, 2011 Background Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins.
Porro Danilo +8 more
doaj +9 more sources
Second generation Pichia pastoris strain and bioprocess designs
Biotechnology for Biofuels and Bioproducts, 2022 Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün +3 more
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Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris. [PDF]
Microb Cell Fact, 2021 Background Protein synthesis is one of the extremely important anabolic pathways in the yeast expression system Pichia pastoris . Codon optimization is a commonly adopted strategy for improved protein expression, although unexpected failures did appear ...
Xu Y +6 more
europepmc +2 more sources
A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System. [PDF]
Molecules, 2019 Cell surface display systems for immobilization of peptides and proteins on the surface of cells have various applications, such as vaccine generation, protein engineering, bio-conversion and bio-adsorption. Though plenty of methods have been established
Li S, Qiao J, Lin S, Liu Y, Ma L.
europepmc +2 more sources
Fine-tuning the P. pastoris iMT1026 genome-scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources. [PDF]
Microb Biotechnol, 2018 The methylotrophic yeast Pichia pastoris (Komagataella spp.) is widely used as cell factory for recombinant protein production. In the past recent years, important breakthroughs in the systems‐level quantitative analysis of its physiology have been ...
Tomàs-Gamisans M, Ferrer P, Albiol J.
europepmc +2 more sources
Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Journal of Fungi, 2023 Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha +4 more
doaj +2 more sources
Journal of Cellular Physiology, 2020 One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts,
Mohsen Karbalaei +2 more
exaly +2 more sources
Synthetic and Systems Biotechnology, 2023 The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter (pAOX1). However, it is difficult to use P.
Xiang Wang +6 more
doaj +2 more sources