Results 71 to 80 of about 25,435,117 (220)

Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris

Bioscience Journal, 2018
Xylanase can hydrolyze xylan for reducing its anti-nutritional impact and improving nutrient availability, so obtaining suitable xylanase to degrade xylan is essential.
Shuli Li, Weiwei Huang, Juan Chang, Ping Wang, Qingqiang Yin, Chaoqi Liu, Erzhu Wang, Fushan Lu   +7 more
doaj   +3 more sources

Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris

Asian Pacific Journal of Tropical Biomedicine, 2017
Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris). Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the
Fernita Puspasari, Riski Dwimalida Putri, Aisyah, Raden Roro Rika Damayanti, Anita Yuwita, Bachti Alisjahbana, Sukwan Handali, Ihsanawati, Dessy Natalia   +8 more
doaj   +1 more source

Insights into tandem dioxygenases—copper radical oxidases in fungal Phytopathogens of the Colletotrichum acutatum species complex

The FEBS Journal, EarlyView.
In Colletotrichum acutatum species complex, conserved ring‐cleavage dioxygenases located adjacent to AA5_2 enzymes were investigated as potential redox partners. Biochemical analyses identified the dioxygenases as intradiol‐cleaving enzymes preferentially oxidizing methyl‐substituted catechols, while AA5_2 enzymes displayed weak alcohol oxidase ...
Radka Končitíková, David Ribeaucourt, Mireille Haon, David Navarro, Elodie Drula, Bastien Bissaro, Jean‐Guy Berrin, Mickael Lafond   +7 more
wiley   +1 more source

R scripts: edgeR analysуs of Capsella bursa-pastoris

, 2016
These scripts were used to perform differential gene expression analysis between and within the genetic groups (ASI, EUR, ME) of Capsella bursa ...
Karl Holm (3300639), Chen, Jun, Jun Chen (4238), Glémin, Sylvain, Lascoux, Martin, Dmytro Kryvokhyzha (3283938), Cornille, Amandine, Martin Lascoux (201001), Wright, Stephen I., Sylvain Glémin (18118), Stephen I. Wright (49030), Lagercrantz, Ulf, Amandine Cornille (165965), Kryvokhyzha, Dmytro, Holm, Karl, Ulf Lagercrantz (395747)   +15 more
core   +1 more source

Neuroanatomy education through the years: Revisiting the teaching slides used by Sir Wilfrid Edward Le Gros Clark

Journal of Anatomy, EarlyView.
This paper examines the life and legacy of British anatomist Sir Wilfrid Le Gros Clark through his neuroanatomical teaching collection at the University of Oxford. The authors catalogued 103 specimens and analyzed selected slides—including cases of multiple sclerosis, amyotrophic lateral sclerosis, tabes dorsalis, syringomyelia, and the pineal gland's ...
Shiva A. Nischal, Kush M. Kale, Katherine Tourigny, Mohammed Abuelem, Gretchen Greene, Rachel Walsh, Zoltán Molnár   +6 more
wiley   +1 more source

Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia pastoris.

, 2005
The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZalphaA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P.
Gutiérrez, J, P. E. Hernandez, J. Gutierrez, R. Criado, L. M. Cintas, Criado, R, C. Herranz, Martín, M, Hernández, PE, Cintas, LM, M. Martin, Herranz, C   +11 more
core   +2 more sources

Natural and CRISPR/Cas9 Editing Variations of Dual‐uORFs Synergistically Increase the Nutritional Value of Silage Maize Feed

Plant Biotechnology Journal, EarlyView.
ABSTRACT The precise enhancement of nutritional quality in silage maize is a core strategy for increasing livestock production efficiency. Through evolutionary analysis of multiple plant species, we identified two functionally synergistic upstream open reading frames (uORF1 and uORF2) within the 5′ untranslated region of the gene encoding GDP‐L ...
Jing Zhou, Xi Wang, Yajing Zhang, Xinhao Luo, Shuyao Quan, Jie Ji, Lixue Sun, Xinyang Chen, Jin Chen, Jianbo Cheng, Jun Fan, Changsheng Li, Beijiu Cheng, Xiaoyu Li   +13 more
wiley   +1 more source

Expression of functional plant lectins in heterologous systems [PDF]

, 2000
The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation.
Raemaekers, Romaan J.M.
core  

Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants

Plant, Cell &Environment, EarlyView.
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn, Fabian B. Haas, Benjamin G. Chavez, Bernd H. Dreyer, Erik V. Reiss, Anne Ziplys, Heiko Weichert, Saskia Hiltemann, José M. Ugalde, Andreas J. Meyer, John C. D'Auria, Stefan A. Rensing, Jos H. M. Schippers   +12 more
wiley   +1 more source

Deglycosylation of recombinant proteins expressed in P. pastoris.

, 2015
(A). SDS-PAGE of the recombinant proteins expressed in P. pastoris with or without digestion of Endo H-P with post-fermentation method. M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 positive control ...
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source