Results 71 to 80 of about 23,995,070 (219)

Natural and CRISPR/Cas9 Editing Variations of Dual‐uORFs Synergistically Increase the Nutritional Value of Silage Maize Feed

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT The precise enhancement of nutritional quality in silage maize is a core strategy for increasing livestock production efficiency. Through evolutionary analysis of multiple plant species, we identified two functionally synergistic upstream open reading frames (uORF1 and uORF2) within the 5′ untranslated region of the gene encoding GDP‐L ...
Jing Zhou   +13 more
wiley   +1 more source

R scripts: edgeR analysуs of Capsella bursa-pastoris

open access: yes, 2016
These scripts were used to perform differential gene expression analysis between and within the genetic groups (ASI, EUR, ME) of Capsella bursa ...
Karl Holm (3300639)   +15 more
core   +1 more source

Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants

open access: yesPlant, Cell &Environment, EarlyView.
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn   +12 more
wiley   +1 more source

Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

open access: yesMicrobial Cell Factories, 2008
Background The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host.
Strange Philip G   +8 more
doaj   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

open access: yesBiotechnology and Bioengineering, Volume 123, Issue 10, Page 2520-2535, October 2026.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek   +3 more
wiley   +1 more source

Expression of functional plant lectins in heterologous systems [PDF]

open access: yes, 2000
The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation.
Raemaekers, Romaan J.M.
core  

List of P. pastoris strains.

open access: yes, 2014
List of P. pastoris strains.
Oded Lewinson (638433)   +9 more
core   +1 more source

Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris

open access: yesElectronic Journal of Biotechnology, 2016
Background: Human is an essential cellular enzyme that is found in all human cells. As this enzyme is upregulated in cancer cells exceedingly, it is used as a target for cancer chemotherapeutic drug development. As such, producing the in-house enzyme for
Ruo Ping Ang   +4 more
doaj   +1 more source

From Circuits to Symphonies: A Systems‐Engineering Blueprint for Multimicrobial Synthetic Biology

open access: yesAdvanced Science, Volume 13, Issue 49, 3 September 2026.
Engineering synthetic microbial consortia requires moving beyond intracellular genetic circuits toward coordinated multicellular design. A modular systems‐engineering framework is presented that integrates communication, temporal coordination, ecological structuring, and predictive modeling to guide application‐dependent consortium design ...
Miguel Fernández‐Niño   +9 more
wiley   +1 more source

Recombinant gelatin and collagen from methylotrophic yeasts [PDF]

open access: yes, 2002
Based on its structural role and compatibility within the human body, collagen is a commonly used biomaterial in medical applications, such as cosmetic surgery, wound treatment and tissue engineering.
Bruin, E.C., de
core  

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