Results 71 to 80 of about 47,430 (223)
BioengineeringEnzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj +1 more source
BMC Biotechnology, 2018 Background The methanol-regulated AOX1 promoter (P AOX1 ) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of P AOX1 restricts
Ching-Hsiang Chang +3 more
doaj +1 more source
Galactose oxidase from Fusarium oxysporum--expression in E. coli and P. pastoris and biochemical characterization. [PDF]
PLoS One, 2014 A gene coding for galactose 6-oxidase from Fusarium oxysporum G12 was cloned together with its native preprosequence and a C-terminal His-tag, and successfully expressed both in Escherichia coli and Pichia pastoris. The enzyme was subsequently purified and characterized.
Paukner R +6 more
europepmc +5 more sources
Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris [PDF]
, 2016 BACKGROUND: Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production.
Jiangchao Qian +7 more
core +1 more source
Adaptive Genomic Divergence Across Altitudes in Capsella bursa‐pastoris
Biological Diversity, EarlyView.Genomic divergence was observed between high‐altitude and low‐altitude populations of Capsella bursa‐pastoris in China, consistent with an important role of ecological factors. Candidate adaptive loci associated with enhanced energy metabolism, photoprotection, and growth plasticity under altitudinal stress were identified.
Lu Liu +4 more
wiley +1 more source
Enhanced thermostability of a
Microbial Cell Factories, 2012 Background Lipase from Rhizopus chinensis is a versatile biocatalyst for various bioconversions and has been expressed at high-level in Pichia pastoris. However, the use of R.
Yu Xiao-Wei +4 more
doaj +1 more source
The power of many: when genetics met yeasts and high‐throughput
Biological Reviews, EarlyView.ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
Influence of pH on cellular growth of Pichia pastoris KM71H by fed-batch process [PDF]
, 2009 Pichia pastoris is a methylotrophic yeast that can be genetically engineered to express proteins for industrial use. One of the most important advantages of protein expression in P.
Bruneska, Danyelly +6 more
core
Pay32p of the Yeast Yarrowia lipolytica Is an Intraperoxisomal Component of the Matrix Protein Translocation Machinery [PDF]
, 1995 Pay mutants of the yeast Yarrowia lipolytica fail to assemble functional peroxisomes. One mutant strain, pay32-1, has abnormally small peroxisomes that are often found in clusters surrounded by membranous material.
Rachubinski, Richard A., +3 more
core +3 more sources
Chemistry – A European Journal, EarlyView.The introduction outlines the review scope. Microbial cell factories as living drugs cover host–gut microbiota, bacteria, yeast, and other microbial systems, with comparative host advantages. Engineering strategies include synthetic circuits, quorum sensing, and memory.
Cemile Elif Özçelik +3 more
wiley +1 more source