Results 91 to 100 of about 25,435,117 (220)

The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product

Journal of Virological Methods, 2004
High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS-CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZalphaA. SDS-PAGE and Western blotting
Han, Xueqing, Bartlam, Mark, Jin, Ying-hua, Liu, Xiangtao, He, Xiaojing, Cai, Xuepeng, Xie, Qingqe, Rao, Zihe   +7 more
openaire   +2 more sources

Optimization of the conditions for recombinant protein secretion in p. pastoris yeast. [PDF]

, 2020
Optimization of the Conditions for Recombinant Protein Secretion in P.
Kireilis, Rokas,
core  

Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris

BioTechniques, 2001
Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of ...
Jon Soderholm, Brooke J. Bevis, Benjamin S. Glick   +2 more
doaj   +1 more source

Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production. [PDF]

Biotechnol Biofuels Bioprod, 2023
Gao L, Meng J, Dai W, Zhang Z, Dong H, Yuan Q, Zhang W, Liu S, Wu X.   +8 more
europepmc   +1 more source

Cloning, Expression, and Functional Characterization of Autoactivated Human Prethrombin-2 Synthetic Gene by Using Pichia pastoris SMD1168 As a Host

Microbiology Indonesia, 2016
Prethrombin-2 is a thrombin precursor that has important role in blood coagulation. It is the smallest precursor which is activated into thrombin by FXa prior to coagulation process.
TOTO SUBROTO, WULAN PERTIWI, MUHAMMAD FADHILLAH, KHOMAINI HASAN, OGI BUDIANTORO, SUTARYA ENUS, SOETIJOSO SOEMITRO   +6 more
doaj   +1 more source

High-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut⁺ bioreactor process under controlled residual methanol concentration. [PDF]

Bioprocess Biosyst Eng, 2022
Bolmanis E, Grigs O, Kazaks A, Galvanauskas V.   +3 more
europepmc   +1 more source

Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris. [PDF]

Microb Cell Fact, 2021
Xu Y, Liu K, Han Y, Xing Y, Zhang Y, Yang Q, Zhou M.   +6 more
europepmc   +1 more source

Recombinant keratinases production by P. pastoris X-33.

, 2013
A: Kinetics of keratinase production by P. pastoris pPICZα-kerAwt, pPICZα-kerAopti1 and pPICZα-kerAopti2. B: Comparative expression of kerAwt, kerAopti1 and kerAopti2 in P. pastoris at 96 h.
Zhiqing Huang (13720), Guoquan Han (385328), Ping Zheng (21877), Jie Yu (150626), Jie Gao (10266), Jun He (46556), Daiwen Chen (326144), Bing Yu (237109), Hong Hu (62885), Xiangbing Mao (147316)   +9 more
core   +1 more source

Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris

Synthetic and Systems Biotechnology
Synthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations in production
Bo Sun, Yu Tan, Nitesh Kumar Mund, Ketao Wang, Lirong Yang, Jianping Wu, Jixing Luo, Yuanqiang Li, Ling Jiang, Hao Fang, Haoran Yu   +10 more
doaj   +1 more source

SYNONYMOUS CONDON USAGE BIAS AND OVEREXPRESSION OF A SYNTHETIC xynB GENE FROM Aspergillus niger NL-1 IN Pichia pastoris

BioResources, 2012
To further improve the expression level of recombinant xylanase in Pichia pastoris, the xynB gene, encoding the mature peptide from Aspergillus niger NL-1, was designed and synthesized based on the synonymous condon bias of P.
Fei Li, Shiyi Yang,, Linguo Zhao,, Qi Li,, Jianjun Pei   +3 more
doaj