Results 91 to 100 of about 20,634,842 (205)
Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris
BioTechniques, 2001 Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of ...
Jon Soderholm +2 more
doaj +1 more source
Nature Biotechnology, 2019 The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, P.
T. Gassler +8 more
semanticscholar +1 more source
Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production. [PDF]
Biotechnol Biofuels Bioprod, 2023 Gao L +8 more
europepmc +1 more source
Microbiology Indonesia, 2016 Prethrombin-2 is a thrombin precursor that has important role in blood coagulation. It is the smallest precursor which is activated into thrombin by FXa prior to coagulation process.
TOTO SUBROTO +6 more
doaj +1 more source
High-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut+ bioreactor process under controlled residual methanol concentration. [PDF]
Bioprocess Biosyst Eng, 2022 Bolmanis E +3 more
europepmc +1 more source
P. pastoris Surface Display Service
The most used yeast is P. pastoris which can reach a much higher cell density than S. cerevisiae in fermentation, as well as can be utilized for preparation of recombinant protein. In order to achieve the high expression of surface display in P. pastoris, a new cell surface display system has been established.
openaire +2 more sources
BioResources, 2012 To further improve the expression level of recombinant xylanase in Pichia pastoris, the xynB gene, encoding the mature peptide from Aspergillus niger NL-1, was designed and synthesized based on the synonymous condon bias of P.
Fei Li, Shiyi Yang, +3 more
doaj
Microbial Cell Factories, 2011 Background FAD dependent glucose dehydrogenase (GDH) currently raises enormous interest in the field of glucose biosensors. Due to its superior properties such as high turnover rate, substrate specificity and oxygen independence, GDH makes its way into ...
Sygmund Christoph +7 more
doaj +1 more source
Application of In-Situ and Soft-Sensors for Estimation of Recombinant P. pastoris GS115 Biomass Concentration: A Case Analysis of HBcAg (Mut+) and HBsAg (MutS) Production Processes under Varying Conditions. [PDF]
Sensors (Basel), 2021 Grigs O, Bolmanis E, Galvanauskas V.
europepmc +1 more source
Cloning of Vibrio cholerae outer membrane protein W in Pichia pastoris
Iranian Journal of Microbiology, 2013 Background and Objective: The outer membrane protein W (ompW) of Vibrio cholerae is involved in stimulating the im- mune response via induction of protective immunity.
Javad Alizadeh +5 more
doaj