Results 91 to 100 of about 20,152,363 (248)

Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

AMB Express, 2019
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda   +7 more
doaj   +1 more source

Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris

Biotechnology for Biofuels, 2021
Background Pichia pastoris (syn. Komagataella phaffii) is an important yeast system for heterologous protein expression. A robust P. pastoris mutant with oxidative and thermal stress cross-tolerance was acquired in our previous study.
Nai-Xin Lin, Rui-Zhen He, Yan Xu, Xiao-Wei Yu   +3 more
doaj   +1 more source

Potato leafroll virus: A re‐emerging threat to sustainable potato production

Annals of Applied Biology, Volume 189, Issue 1, July 2026.
Potato leafroll virus (PLRV) is a positive‐strand RNA virus. Virus particles of PLRV are isometric, 24 nm in diameter. PLRV is the causal agent of leafroll disease in potato (Solanum tuberosum L.) which causes significant losses in yield and quality of potato tubers.
Eugene V. Ryabov, Andrew Love, Lesley Torrance   +2 more
wiley   +1 more source

An N‐acetyltransferase‐MAPK fusion protein modulates developmental reprogramming in Physcomitrium patens

New Phytologist, Volume 251, Issue 1, Page 321-339, July 2026.
Summary We discovered a previously uncharacterized moss‐specific protein, Rosetta NATD‐MAPK 1 (RAK1) in Physcomitrium patens, which uniquely integrates MAP kinase (MAPK)‐dependent signaling with N‐acetyltransferase activity. Through phenotypical and biochemical analyses, we characterized RAK1 function in the regulation of the 2D‐to‐3D growth transition.
Cloe de Luxán‐Hernández, Thomas J. Ammitsøe, Jakob V. Kanne, Sabrina Stanimirovic, Rocío Redondo‐Rodríguez, Milena E. Roux, Liechi Zhang, Zoe Weeks, Michael Schutzbier, Gerhard Dürnberger, Elisabeth Roitinger, Oliver Spadiut, Masaki Ishikawa, Mitsuyasu Hasebe, Laura A. Moody, Yasin F. Dagdas, Eleazar Rodriguez, Morten Petersen   +17 more
wiley   +1 more source

Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris

, 2011
Includes bibliographical references (leaves 98-103).The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics.
Bhardwaj, Vinayak
core  

Co-fermentaion the phytase expressing P. pastoris and Endo H-P expressing P. pastoris with various initial ratio of cells innoculated in BMMY medium.

, 2015
M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 phytase expressed in P. pastoris; Lane 2–8 EndoH-P-expressing P. pastoris co-fermentated with phytase-expressing P. pastoris with inoculation ratio of 1:10, 1:25,
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source

Results of screening of culture supernatants of P. pastoris X-33_pPICZα/XynT6.

, 2022
Results of screening of culture supernatants of P. pastoris X-33_pPICZα/XynT6.
Assel Kiribayeva (12257166), Birzhan Mukanov (12257169), Zhiger Akishev (12257175), Bekbolat Khassenov (5635241), Yerlan Ramankulov (5635238), Dmitriy Silayev (12257172)   +5 more
core   +1 more source

Exploring Diverse Glycone Preferences of Aspergillus rutinosidases

Advanced Synthesis &Catalysis, Volume 368, Issue 11, 3 June 2026.
Rutinosidases from Aspergillus niger (AnRut) and A. oryzae (AoRut) catalyze hydrolysis and transglycosylation with non‐natural glycosides as donors, enabling glycosylation of alcoholic and phenolic acceptors. AnRut exhibits broad glycone promiscuity, while AoRut has a narrower substrate scope.
Jitka Brouzdová, Lucie Petrásková, Katerina Brodsky, Natalia Kulik, Helena Pelantová, Martina Hurtová, Andrea Vopálenská, Kristýna Slámová, Pavla Bojarová, Vladimír Křen   +9 more
wiley   +1 more source

Large-scale functional expression of WT and truncated human adenosine A_2A receptor in Pichia pastoris bioreactor cultures

Microbial Cell Factories, 2008
Background The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host.
Strange Philip G, Rana Rohini, Martinez Magdalena, Ruprecht Jonathan, Fiez-Vandal Cédric, Gras Adrien, Singh Shweta, Wagner Renaud, Byrne Bernadette   +8 more
doaj   +1 more source

Deglycosylation of recombinant proteins expressed in P. pastoris.

, 2015
(A). SDS-PAGE of the recombinant proteins expressed in P. pastoris with or without digestion of Endo H-P with post-fermentation method. M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 positive control ...
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source