Results 111 to 120 of about 47,430 (223)

A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation [PDF]

open access: yes, 2016
Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how ...
Jimenez del Val, I   +2 more
core   +1 more source

Anholocyclic overwintering of Myzus persicae: Host plant selection as a driver for aphid performance and survival

open access: yesAnnals of Applied Biology, Volume 188, Issue 3, Page 732-744, May 2026.
The green peach aphid, Myzus persicae, is the main virus vector in sugar beet. We examine its performance on potential winter hosts as well as the host selection and overwintering success of anholicyclic aphids in order to improve forecasting models for virus outbreaks through targeted monitoring of suitable winter hosts. Abstract Virus yellows (VY) is
Mohamed Matared   +2 more
wiley   +1 more source

Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production. [PDF]

open access: yesBiotechnol Biofuels Bioprod, 2023
Gao L   +8 more
europepmc   +1 more source

Impact of the quality of organic amendments on size and composition of the weed seed bank [PDF]

open access: yes, 2011
In addition to improving the soil quality, organic amendments of soils may affect weed seed survival, emergence, growth and reproduction. This study evaluated the effects of applications of different qualities of organic amendments on size and ...
Bulcke, Robert   +5 more
core   +1 more source

The Hemoprotein Hhy1 Promotes Heme‐Dependent Catalase Activity of Ctt1

open access: yesMolecular Microbiology, Volume 125, Issue 5, Page 389-411, May 2026.
A Schizosaccharomyces pombe strain deficient in heme (hem1Δ) and siderophore (Fc) biosynthesis, as well as high‐affinity iron (Fe) uptake (blue), was used to uncover the hemoprotein Hhy1 that promotes the heme‐dependent activity of the catalase Ctt1. In contrast, hhy1Δ cells exhibit reduced Ctt1 activity and increased sensitivity to oxidative stress ...
Tobias Vahsen   +3 more
wiley   +1 more source

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

open access: yesPLoS ONE, 2011
Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent drug efflux pump involved in multidrug resistance of ...
Jiangping Bai   +10 more
openaire   +4 more sources

Cloning, Expression, and Functional Characterization of Autoactivated Human Prethrombin-2 Synthetic Gene by Using Pichia pastoris SMD1168 As a Host

open access: yesMicrobiology Indonesia, 2016
Prethrombin-2 is a thrombin precursor that has important role in blood coagulation. It is the smallest precursor which is activated into thrombin by FXa prior to coagulation process.
TOTO SUBROTO   +6 more
doaj   +1 more source

Yeasts [PDF]

open access: yes, 2018
Yeasts are a group of eukaryotic microfungi with a well-defined cell wall whose growth is either entirely unicellular or a combination of hyphal and unicellular reproduction. The approximately 1500 known yeast species belong to two distinct fungal phyla,
Lachance, Marc-Andre, Walker, Graeme M.
core   +2 more sources

Engineering of 10‐Deacetylbaccatin III‐10‐β‐O‐Acetyltransferase From Taxus Species for Efficient Acetylating Non‐Natural Substrates Into Taxol in Nicotiana benthamiana

open access: yesPlant Biotechnology Journal, Volume 24, Issue 5, Page 3308-3323, May 2026.
ABSTRACT 7‐β‐xylosyl‐10‐deacetyltaxol (XDT) is much more abundant than the anticancer drug Taxol in Taxus species and is usually regarded as the by‐product of Taxol. It could be enzymatically transformed into 10‐deacetyltaxol (DT), and the latter could be further converted into Taxol.
Tian‐Jiao Chen   +5 more
wiley   +1 more source

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