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High-Accuracy Serodiagnosis of African Swine Fever Using P72 and P30-Based Lateral Flow Assays: A Validation Study with Field Samples in Thailand [PDF]

Veterinary Sciences
African Swine Fever (ASF) control is severely hampered by the reliance on slow, laboratory-bound diagnostics. While rapid, field-deployable lateral flow assays (LFAs) are urgently needed, the comparative performance of key single-antigen targets remains ...
Nitipon Srionrod, Supphathat Wutthiwitthayaphong, Teera Nipakornpun, Sakchai Ruenphet   +3 more
doaj   +2 more sources

Molecular epidemiology, spatiotemporal patterns, and economic impact of African swine fever in Meghalaya, India [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundAfrican swine fever (ASF), caused by Asfivirus haemorrhagiae, entered India through its northeastern frontiers in 2020. Following the initial outbreaks in Arunachal Pradesh and Assam, the next state to report ASF was Meghalaya, a tribal ...
Arockiasamy Arun Prince Milton, Kandhan Srinivas, Sabia Khan, Archana Thakur, Zakir Hussain, M. Saminathan, Sandeep Ghatak, G. Bhuvana Priya, Hosterson Kylla, Laureata Dkhar, Arnab Sen, Kekungu-u Puro, Samir Das   +12 more
doaj   +2 more sources

P54. Female Genital Mutilation Reconstruction: Surgical Technique

Plastic and Reconstructive Surgery, Global Open
PURPOSE: Reconstruction after abdominoperineal resection (APR) typically utilizes primary closure, locoregional myocutaneous flaps (gracilis or vertical rectus myocuta-neous (VRAM)), or omental flaps. While flap coverage is considered superior to primary
Sarah Barnett, BA, Elizabeth Card, MD, Ivona Percec, MD, PhD   +2 more
doaj   +2 more sources

Baculovirus-Displayed ASFV Epitope-Composite Protein Elicits Potent Immune Responses [PDF]

Microorganisms
African swine fever (ASF), caused by the African swine fever virus (ASFV), is an acute, febrile, highly contagious, and lethal disease that poses a severe threat to the global pig farming industry.
Wenkai Zhang, Xing Yang, Xingyu Chen, Jiaxin Jin, Yuanyuan Zhang, Lele Gong, Shuai Zhang, Xuyang Zhao, Yongkun Du, Yanan Wu, Aijun Sun, Guoqing Zhuang   +11 more
doaj   +2 more sources

Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate

Viruses, 2022
African swine fever (ASF) is a highly contagious hemorrhagic disease of pigs, posing a significant threat to the world pig industry. Several researchers are investigating the possibilities for developing a safe and efficient vaccine against ASF.
Lulu Wang, Dan Fu, Weldu Tesfagaber, Fang Li, Weiye Chen, Yuanmao Zhu, Encheng Sun, Wan Wang, Xijun He, Yu Guo, Zhigao Bu, Dongming Zhao   +11 more
doaj   +1 more source

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus

Frontiers in Cellular and Infection Microbiology, 2022
In recent years, African swine fever (ASF) has caused a devastating blow to the swine industry globally. Since no effective vaccine is available, strict biosafety measures and rapid diagnosis are the most effective strategies for ASF control. ASFV p30 is
Jingjing Ding, Jifei Yang, Daoyuan Jiang, Yanyang Zhou, Chenxi Li, Chenxi Li, Chenxi Li, Yanhua Li, Yanhua Li, Yanhua Li   +9 more
doaj   +1 more source

Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice

AMB Express, 2021
African classical swine fever virus (ASFV) has spread seriously around the world and has dealt with a heavy blow to the pig breeding industry due to the lack of vaccines. In this study, we produced recombinant Lactobacillus plantarum (L.
Xiao-Lei Chen, Jun-Hong Wang, Wei Zhao, Chun-Wei Shi, Kai-Dian Yang, Tian-Ming Niu, Gui-Lian Yang, Xin Cao, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Yan Zeng, Nan Wang, Wen-Tao Yang, Chun-Feng Wang   +14 more
doaj   +1 more source

Pelvic Examination in Undergraduate Medical Education: A Scoping Review. [PDF]

Clin Teach
ABSTRACT Background Pelvic examination is a core clinical skill in undergraduate medical education, requiring integration of technical competence with communication, consent and patient‐centred care. Despite its importance, opportunities to learn pelvic examination are variable, and educational approaches differ widely.
Boutrous C, Rao S, Bluemel AH, Brown MEL, Stelling H.   +4 more
europepmc   +2 more sources

Dietary effects of protein and lipid levels on growth performance and flesh quality of large-size largemouth bass (Micropterus salmoides)

Aquaculture Reports, 2023
The aim of this trial was to investigate the effects of dietary protein and lipid levels on growth performance and flesh quality of largemouth bass (Micropterus salmoides).
Yunfeng Chen, Hang Yang, Beibei Guo, Xiaoqin Li, Xiangjun Leng   +4 more
doaj   +1 more source

Activation of the Plasmodium Egress Effector Subtilisin-Like Protease 1 Is Mediated by Plasmepsin X Destruction of the Prodomain

mBio, 2023
Following each round of replication, daughter merozoites of the malaria parasite Plasmodium falciparum escape (egress) from the infected host red blood cell (RBC) by rupturing the parasitophorous vacuole membrane (PVM) and the RBC membrane (RBCM).
Sumit Mukherjee, Armiyaw S. Nasamu, Kelly C. Rubiano, Daniel E. Goldberg   +3 more
doaj   +1 more source