Results 31 to 40 of about 47,490 (285)

SMRT-Cappable-seq reveals complex operon variants in bacteria

open access: yesNature Communications, 2018
Fragmentation of transcripts can hide operon complexity in genome-wide transcriptome sequencing. Here the authors sequenced a prokaryotic transcriptome using PacBio and provide insights into operon structure and read-through at termination sites.
Bo Yan   +3 more
doaj   +1 more source

PacBio sequencing raw data

open access: yes, 2022
Sequencing ...
openaire   +1 more source

Chromosomal-level assembly of the Asian Seabass genome using long sequence reads and multi-layered scaffolding [PDF]

open access: yes, 2016
We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish ...
A Bairoch   +190 more
core   +5 more sources

Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate

open access: yesHuman Vaccines & Immunotherapeutics, 2020
Leptospira interrogans serogroup Canicola is one of the most important pathogens causing leptospirosis and is used as a vaccine strain of the current Chinese human leptospirosis vaccine. To characterize leptospiral pathogens, L.
Ruipeng Zhang   +6 more
doaj   +1 more source

IPA assembly for PacBio Hifi reads v1

open access: yes, 2021
This protocol is regarding the assembly of plant genomes using HiFi data (PacBio Sequel II) with Improved Phase Assembly (IPA) tool.
Priyanka Sharma   +11 more
openaire   +1 more source

Amplification and adaptation of centromeric repeats in polyploid switchgrass species. [PDF]

open access: yes, 2018
Centromeres in most higher eukaryotes are composed of long arrays of satellite repeats from a single satellite repeat family. Why centromeres are dominated by a single satellite repeat and how the satellite repeats originate and evolve are among the most
Braz, Guilherme T   +10 more
core   +2 more sources

Pacbio Sequencing of PLC/PRF/5 Cell Line and Clearance of HBV Integration Through CRISPR/Cas-9 System

open access: yesFrontiers in Molecular Biosciences, 2021
The integration of HBV DNA is one of the carcinogenic mechanisms of HBV. The clearance of HBV integration in hepatocyte is of great significance to cure chronic HBV infection and thereby prevent the occurrence of HBV-related hepatocellular carcinoma (HCC)
Chia-Chen Chen   +8 more
doaj   +1 more source

The complete mitochondrial genome of the Antarctic marbled rockcod, Notothenia rossii (Perciformes, Nototheniidae)

open access: yesMitochondrial DNA. Part B. Resources, 2020
The complete mitochondrial genome of Notothenia rossii was obtained using PacBio Sequel long-read sequencing platform. The mitogenome of N. rossii was circular form and 18,274 bp long, which consists of 13 protein-coding genes, 24 tRNAs, 2 rRNAs, and non-
Euna Jo   +7 more
doaj   +1 more source

Complete Genome and Plasmid Sequences of Staphylococcus aureus EDCC 5055 (DSM 28763), Used To Study Implant-Associated Infections [PDF]

open access: yes, 2017
Staphylococcus aureus EDCC 5055 (DSM 28763) is a human clinical wound isolate intensively used to study implant-associated infections in rabbit and rat infection models.
Alt, Volker   +6 more
core   +1 more source

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

open access: yesMicroorganisms, 2020
Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both
Vyacheslav Toropov   +4 more
doaj   +1 more source

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