Results 41 to 50 of about 16,162 (223)

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, EarlyView.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis [PDF]

Applied and Environmental Microbiology, 2002
ABSTRACT The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular
Bermudez Humaran, Luis, Langella, Philippe, Miyoshi, A, Gruss, Alexandra, Guerra, R Tamez, Montes de Oca-Luna, Roberto, Le Loir, Yves   +6 more
openaire   +4 more sources

Human papillomavirus E7 proteins stimulate proliferation independently of their ability to associate with retinoblastoma protein [PDF]

Oncogene, 2000
Studies on human papillomavirus type 16 have demonstrated that the product of the early gene, E7, plays a key role in the immortalization and malignant transformation of the host cell. Several of the biological activities of HPV16 E7 are mediated by inactivation of the members of the pocket protein family, pRb, p107 and p130.
S, Caldeira, E M, de Villiers, M, Tommasino   +2 more
openaire   +2 more sources

Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Scientific Reports, 2017
The human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for ...
Hanxiang Chen, Qishu Zhang, Lijun Qiao, Xueli Fan, Weifang Zhang, Weiming Zhao, Jason J. Chen   +6 more
doaj   +1 more source

Evaluation of HPV16 E7 expression in head and neck carcinoma cell lines and clinical specimens

Scientific Reports, 2020
Human papillomavirus (HPV) 16 infection in the oropharynx is one of the major risk factors for oropharyngeal carcinoma. Although the HPV E6 and E7 proteins are known to have a role in head and neck carcinogenesis, whether their expression is maintained ...
Koji Kitamura, Keisuke Nimura, Rie Ito, Kotaro Saga, Hidenori Inohara, Yasufumi Kaneda   +5 more
doaj   +1 more source

Genomic organization of a Gamma-6 papillomavirus metagenomic discovered from vaginal swab samples of Chinese pregnant women

Virology Journal, 2020
A complete genome sequence of human papillomaviruses (HPV) named as HPV-ujs-21015 was determined by viral metagenomic and PCR methods. The complete genome is 7354 bp in length with GC content of 41.7%, of which the genome was predicted to contain six ...
Yu Ling, Jianqiang Wang, Jun Yin, Jianpu Xu, Yufan Wu, Rui Zhou, Juan Lu, Shixing Yang, Xiaochun Wang, Quan Shen, Wen Zhang   +10 more
doaj   +1 more source

HPV types 16/18 L1 E6 and E7 proteins seropositivity and cervical cancer risk in HIV-positive and HIV-negative black South African women

Infectious Agents and Cancer, 2022
Background In populations with high rates of human immunodeficiency virus (HIV)-coinfection, the nature of the relationship between human papillomavirus (HPV)-16 and -18 (L1, E6 and E7) antibodies and cervical cancer is still uncertain.
Mwiza Gideon Singini, Elvira Singh, Debbie Bradshaw, Wenlong Carl Chen, Melitah Motlhale, Abram Bunya Kamiza, Chantal Babb de Villiers, Mazvita Muchengeti, Christopher G. Mathew, Robert Newton, Noemi Bender, Tim Waterboer, Freddy Sitas   +12 more
doaj   +1 more source

Mutations of the human papillomavirus type 16 E7 gene that affect transformation, transactivation and phosphorylation by the E7 protein

Journal of General Virology, 1990
The human papillomavirus type 16 (HPV-16) E7 gene cooperates with an activated ras oncogene to transform primary rodent cells and is important in the immortalization of cervical keratinocytes. We have generated a series of point mutations within the E7 gene and show that mutation of residues serine 31 and serine 71 affect the phosphorylation of the E7 ...
A, Storey, N, Almond, K, Osborn, L, Crawford   +3 more
openaire   +2 more sources

Identification of human papillomavirus DNA in cutaneous lesions of Cowden syndrome [PDF]

, 2003
Background: Cowden syndrome (CS) or multiple hamartoma syndrome is a cancer-associated genodermatosis inherited in an autosomal dominant pattern. One of the diagnostic criteria is facial papules which are felt to be trichilemmomas, benign hair follicle ...
Schaller, Jörg, Burgdorf, Walter H. C., Rohwedder, A., Lautenschlager, Stephan, Itin, Peter H.   +4 more
core   +1 more source

HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells

Open Medicine, 2022
Cervical cancer is the fourth most common cancer and the fourth leading cause of cancer death in women. Human papillomavirus (HPV16) E6/E7 heterogenous expression in C33A cells increased the mRNA and protein levels of KIF2A, while siRNA deletion of ...
Wang Yuyan, Wang Jinfeng, Zhao Anqi, Huang Xin, Zhang Xin   +4 more
doaj   +1 more source