Results 1 to 10 of about 106,664 (304)

Maintenance of Fluorescence During Paraffin Embedding of Fluorescent Protein-Labeled Specimens [PDF]

Frontiers in Neuroscience, 2019
Paraffin embedding is widely used in microscopic imaging for preparing biological specimens. However, owing to significant fluorescence quenching during the embedding process, it is not compatible with fluorescent-labeling techniques, such as transgenic ...
Ouyang Zhanmu, Ouyang Zhanmu, Peilin Zhao, Peilin Zhao, Yang Yang, Yang Yang, Xiaoquan Yang, Xiaoquan Yang, Xiaoquan Yang, Hui Gong, Hui Gong, Hui Gong, Xiangning Li, Xiangning Li, Xiangning Li   +14 more
doaj   +2 more sources

Paraffin embedding of the whole human cerebral hemisphere to assess arterial distribution territories [PDF]

EXCLI Journal : Experimental and Clinical Sciences
Commonly used to decode the human brain's structural complexity, ex vivo dissection focuses on a given structure or region but cannot depict the whole brain organization (for example, its arterial distribution territories).
Mykyta Smirnov, Frédéric Andersson, Laurent Barantin, Igor Lima Maldonado, Christophe Destrieux   +4 more
doaj   +2 more sources

Cyclooxygenase-2 and von Willebrand factor—an immunohistochemical study of the equine foot with and without laminitis, post-mortem perfused with paraffin oil [PDF]

Frontiers in Veterinary Science
ObjectiveEquine laminitis is a complex and potentially fatal disease characterized by severe vascular and inflammatory alterations within the equine foot.
Bianca A. Underberg, Bianca A. Underberg, Elke Van der Vekens, Barbara Drews, Sabine Kaessmeyer   +4 more
doaj   +2 more sources

Modified plasma-thrombin method using patient-derived plasma for cell block preparation in endobronchial ultrasound–guided transbronchial fine-needle aspiration [PDF]

Journal of Pathology and Translational Medicine
Background The plasma-thrombin method, which uses expired blood bank plasma as an ancillary component, has been widely used in cell block (CB) preparation.
Xizhe Zhang, Chunli Tang, Yingying Gu, Zeyun Lin, Shiqi Tang, Anzi Tan, Mengshi Li, Zhucheng Chen, Yuying Chen, Shi-yue Li, Juhong Jiang   +10 more
doaj   +2 more sources

Protocol for the establishment and morphological characterization of long-term cultivated murine cerebral organoids [PDF]

STAR Protocols
Summary: Murine cerebral organoids provide a rapid and reproducible in vitro system that recapitulates key aspects of neurogenesis. While human cerebral organoid protocols are well established, methods for non-human models remain limited.
Issam El-Debs, Michael R. Knittler, Thomas C. Mettenleiter, John O. Mason, Julia Sehl-Ewert   +4 more
doaj   +2 more sources

Rapid en-bloc hematoxylin-eosin staining for human lung cancer tissue for fluorescence micro-optical sectioning tomography

Frontiers in Physics, 2023
Objective: To establish a rapid and effective method for en-bloc hematoxylin-eosin (HE) staining and paraffin embedding of human lung cancer and paracancerous tissues which can be applied to fluorescence micro-optical sectioning tomography (fMOST ...
Yun Ding, Huaye Wei, Xin Liu, Meilin Xu, Daqiang Sun, Daqiang Sun, Ting Li, Xin Li   +7 more
doaj   +1 more source

Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy

Scientific Reports, 2021
Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested.
Aiste Vitkunaite, Aida Laurinaviciene, Benoit Plancoulaine, Allan Rasmusson, Richard Levenson, Michael Shribak, Arvydas Laurinavicius   +6 more
doaj   +1 more source

An optimized protocol using Steedman's wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flower buds of cucumber

Journal of Integrative Agriculture, 2023
In situ mRNA hybridization (ISH) is a powerful tool for examining the spatiotemporal expression of genes in shoot apical meristems and flower buds of cucumber.
Cui WANG, Jin-jing SUN, Xue-yong YANG, Li WAN, Zhong-hua ZHANG, Hui-min ZHANG   +5 more
doaj   +1 more source

Expression of p53 and Ki-67 proteins in patients with increasing severity and duration of pterygium

Indian Journal of Ophthalmology, 2021
Purpose: Pterygium is a triangular fibrovascular subepithelial ingrowth of degenerative bulbar conjunctival tissue over the cornea. It is now considered to be a result of uncontrolled cellular proliferation as overexpression of p53 protein and Ki-67 ...
Mood Mahesh, Sanjeev K Mittal, Sanjeev Kishore, Anupam Singh, Neeti Gupta, Rimpi Rana   +5 more
doaj   +1 more source

Standard techniques for formalin-fixed paraffin-embedded tissue: A Pathologist’s perspective [PDF]

Iraqi Journal of Veterinary Sciences, 2021
Histopathology considered as an essential keystone to understanding diseases on a cellular level, without examining affected tissues and cells; will lack the accurate diagnosis. The most common protocol that used worldwide for histopathology is termed as
Hadil B. Al-Sabaawy, Asseel M. Rahawi, Saevan S. Al-Mahmood   +2 more
doaj   +1 more source