Results 1 to 10 of about 6,562 (141)

Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps [PDF]

Sensors, 2023
A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li, Huiying Gong, Jinyu Qiu, Ruimin Li, Qili Zhao, Xin Zhao, Mingzhu Sun   +6 more
doaj   +2 more sources

Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat [PDF]

Frontiers in Physiology, 2020
Mitochondria convert the chemical energy of metabolic substrates into adenosine triphosphate (ATP) and heat. Although ATP production has become a focal point of research in bioenergetics, mitochondrial thermogenesis is also crucial for energy metabolism.
Ambre M. Bertholet, Yuriy Kirichok
doaj   +2 more sources

There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments

Frontiers in Molecular Neuroscience, 2022
Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius, Alison Obergrussberger, Edward S. A. Humphries, Stephanie Scholz, Ilka Rinke-Weiss, Tom A. Goetze, Nina Brinkwirth, Maria Giustina Rotordam, Tim Strassmaier, Aaron Randolph, Søren Friis, Aiste Liutkute, Aiste Liutkute, Aiste Liutkute, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Niels Voigt, Niels Voigt, Niels Voigt, Niels Fertig   +20 more
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Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts

Bio-Protocol, 2021
The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry, Mighten Yip, Ilya Kolb, William Stoy, Mercedes Gonzalez, Craig Forest   +5 more
doaj   +1 more source

Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes

Frontiers in Physiology, 2023
Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma, Joanne G. Ma, Jamie I. Vandenberg, Jamie I. Vandenberg, Chai-Ann Ng, Chai-Ann Ng   +5 more
doaj   +1 more source

Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice

Bio-Protocol, 2023
Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess, Helmut Wratil, Peter Kloppenburg   +2 more
doaj   +1 more source

Automatic deep learning-driven label-free image-guided patch clamp system

Nature Communications, 2021
Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos, Gáspár Oláh, Tamas Balassa, Norbert Mihut, Márton Rózsa, Attila Ozsvár, Ervin Tasnadi, Pál Barzó, Nóra Faragó, László Puskás, Gábor Molnár, József Molnár, Gábor Tamás, Peter Horvath   +13 more
doaj   +1 more source

Datasets for calcium dynamics comparison between the whole-cell and a β-escin based perforated patch configuration in brain slices from adult mice

Data in Brief, 2021
Multiple processes shape calcium signals in neurons. The spatial and temporal dynamics of these signals are determined by various cellular parameters, including the calcium influx, calcium buffering, and calcium extrusion.
Simon Hess, Christophe Pouzat, Peter Kloppenburg   +2 more
doaj   +1 more source

Deep learning-based real-time detection of neurons in brain slices for in vitro physiology

Scientific Reports, 2021
A common electrophysiology technique used in neuroscience is patch clamp: a method in which a glass pipette electrode facilitates single cell electrical recordings from neurons.
Mighten C. Yip, Mercedes M. Gonzalez, Christopher R. Valenta, Matthew J. M. Rowan, Craig R. Forest   +4 more
doaj   +1 more source

Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices

STAR Protocols, 2021
Summary: Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current ...
Kevin M. Manz, Justin K. Siemann, Douglas G. McMahon, Brad A. Grueter   +3 more
doaj   +1 more source