Results 11 to 20 of about 6,562 (141)
, 2013 Sperm intracellular pH and calcium concentration ([Ca(2+)]i) are two central factors that control sperm activity within the female reproductive tract. As such, the ion channels of the sperm plasma membrane that alter intracellular sperm [Ca(2+)] and pH play important roles in sperm physiology and the process of fertilization. Indeed, sperm ion channels
Polina, Lishko +3 more
openaire +2 more sources
Stem Cell Research, 2019 Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology.
Wener Li +6 more
doaj +1 more source
Wellcome Open Research, 2021 Automated patch-clamp platforms are widely used and vital tools in both academia and industry to enable high-throughput studies such as drug screening. A leak current to ground occurs whenever the seal between a pipette and cell (or internal solution and
Chon Lok Lei +7 more
doaj +1 more source
Early identification of hERG liability in drug discovery programs by automated patch clamp
Frontiers in Pharmacology, 2014 Blockade of the cardiac ion channel coded by hERG can lead to cardiac arrhythmia, which has become a major concern in drug discovery and development.
Timm eDanker, Clemens eMöller
doaj +1 more source
PLoS ONE, 2021 Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan +3 more
doaj +1 more source
Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization
eLife, 2021 The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons.
Brian R Lee +27 more
doaj +1 more source
Bioengineering, 2020 Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C.
Jann Harberts +4 more
doaj +1 more source
Preparation of Giant Escherichia coli spheroplasts for Electrophysiological Recordings
Bio-Protocol, 2021 Prokaryotic ion channels have been instrumental in furthering our understanding of many fundamental aspects of ion channels’ structure and function. However, characterizing the biophysical properties of a prokaryotic ion channel in a native membrane ...
Yihao Jiang, Vinay Idikuda, Baron Chanda
doaj +1 more source
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience
Frontiers in Neural Circuits, 2015 Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal ...
Can eTao +3 more
doaj +1 more source
Scientific Reports, 2021 The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach +9 more
doaj +1 more source