Results 21 to 30 of about 6,562 (141)

Whole-cell and Perforated Patch-clamp Recordings from Acutely-isolated Murine Sino-atrial Node Cells

Bio-Protocol, 2020
Cardiac pacemaker cells of the sino-atrial node are responsible for the initiation of the heart beat and express an array of ion channels. The patch-clamp technique is the gold standard method for investigating the function of ion channels expressed in ...
Qadeer Aziz, Muriel Nobles, Andrew Tinker   +2 more
doaj   +1 more source

A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time

Frontiers in Physiology, 2018
An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen, Nadine Becker, Sonja Stoelzle-Feix, Alison Obergrussberger, Marc A. Vos, Toon A. B. van Veen, Niels Fertig, Teun P. de Boer   +7 more
doaj   +1 more source

Scorpion Venom peptide, AGAP inhibits TRPV1 and potentiates the analgesic effect of lidocaine

Heliyon, 2021
The current study was designed to test the hypothesis that BmK AGAP (AGAP) potentiates the analgesic effect of lidocaine. The chronic constrictive injury was performed on 72 rats to induce a rapid onset and long-lasting pain.
Sylvanus Kampo, Yong Cui, Jiachuan Yu, Thomas Winsum Anabah, Aglais Arredondo Falagán, Marcel Tunkumgnen Bayor, Qing-Ping Wen   +6 more
doaj   +1 more source

Exploring the Coordination of Cardiac Ion Channels With Action Potential Clamp Technique

Frontiers in Physiology, 2022
The patch clamp technique underwent continual advancement and developed numerous variants in cardiac electrophysiology since its introduction in the late 1970s. In the beginning, the capability of the technique was limited to recording one single current
Balázs Horváth, Norbert Szentandrássy, Norbert Szentandrássy, Csaba Dienes, Zsigmond M. Kovács, Péter P. Nánási, Péter P. Nánási, Ye Chen-Izu, Leighton T. Izu, Tamas Banyasz   +9 more
doaj   +1 more source

Whole-GUV patch-clamping [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems.
Garten, Matthias, Mosgaard, Lars D., Bornschlögl, Thomas, Dieudonné, Stéphane, Bassereau, Patricia, Toombes, Gilman E. S.   +5 more
openaire   +3 more sources

Electrophysiological Properties of Substantia Gelatinosa Neurons in the Preparation of a Slice of Middle-Aged Rat Spinal Cord

Frontiers in Aging Neuroscience, 2021
A patch-clamp recording in slices generated from the brain or the spinal cord has facilitated the exploration of neuronal circuits and the molecular mechanisms underlying neurological disorders.
Yang Li, Shanchu Su, Jiaqi Yu, Minjing Peng, Shengjun Wan, Changbin Ke   +5 more
doaj   +1 more source

Protocol to prepare mouse spinal cord for patch-clamp and histology experiments

STAR Protocols, 2023
Summary: The spinal cord is a part of the central nervous system located within the spinal canal of the vertebrae. Here, we present a protocol to prepare mouse spinal cord sections for patch-clamp and histology experiments.
Sang-Hyeon Ju, Jong-Woo Sohn
doaj   +1 more source

The application of the Escherichia coli giant spheroplast for drug screening with automated planar patch clamp system

Biotechnology Reports, 2015
Kv2.1, the voltage-gated ion channel, is ubiquitously expressed in variety of tissues and dysfunction of this ion channel is responsible for multiple diseases. Electrophysiological properties of ion channels are so far characterized with eukaryotic cells
Kyoko Kikuchi, Mika Sugiura, Chizuko Nishizawa-Harada, Tadashi Kimura   +3 more
doaj   +1 more source

Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves

STAR Protocols, 2021
Summary: The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves.
Hirosato Kanda, Sotatsu Tonomura, Yi Dai, Jianguo G. Gu   +3 more
doaj   +1 more source

Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes

Frontiers in Physiology, 2023
Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk, Arie O. Verkerk, Ronald Wilders   +2 more
doaj   +1 more source