Results 31 to 40 of about 6,562 (141)
A transistor model for the cystic fibrosis transmembrane conductance regulator
Biophysical Reports, 2023 In this paper we present a transistor circuit model for cystic fibrosis transmembrane conductance regulator (CFTR) that seeks to map the functional form of CFTR both in wild type and mutants.
William D. Hunt +7 more
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Patch clamp-assisted single neuron lipidomics
Scientific Reports, 2017 Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution.
Collin B. Merrill +6 more
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Dendritic patch-clamp recording
Nature Protocols, 2006 The patch-clamp technique allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels. Most patch-clamp recordings from neurons have been made from the soma, the largest structure of individual neurons, while their dendrites, which form the majority of the surface area and receive most of ...
Davie, Jenny +6 more
openaire +4 more sources
Nanotechnology: new opportunities for the development of patch‐clamps
Journal of Nanobiotechnology, 2021 The patch-clamp technique is one of the best approaches to investigate neural excitability. Impressive improvements towards the automation of the patch-clamp technique have been made, but obvious limitations and hurdles still exist, such as ...
Jia Gao +4 more
doaj +1 more source
Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm
Biophysical Reports, 2022 The closing of the gated ion channel in the cystic fibrosis transmembrane conductance regulator can be categorized as nonpermissive to reopening, which involves the unbinding of ADP or ATP, or permissive, which does not.
Alexander S. Moffett +6 more
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SensorsThe patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu +5 more
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Membranes, 2016 Mechanosensitive (MS) channels are ubiquitous molecular force sensors that respond to a number of different mechanical stimuli including tensile, compressive and shear stress.
Omid Bavi +5 more
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STAR Protocols, 2023 Summary: Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection.
Tori Lhomme, Vincent Prévot
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Equilibrium Research, 2008 The patch clamp technique enables the analyses of various ion channel characteristics of individual cells in vitro. The most important difference from previous techniques is that the microelectrode (pipette) is firmly attached to the cell surface instead of being inserted into the cells, and the so-called "giga-ohm seal" is attained. Usually, the whole
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Evaluation of Optogenetic Electrophysiology Tools in Human Stem Cell-Derived Cardiomyocytes
Frontiers in Physiology, 2017 Current cardiac drug safety assessments focus on hERG channel block and QT prolongation for evaluating arrhythmic risks, whereas the optogenetic approach focuses on the action potential (AP) waveform generated by a monolayer of human cardiomyocytes ...
Susann Björk +7 more
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