Results 211 to 220 of about 38,690 (242)

Perforated Patch-Clamp Technique

2003
Application of the conventional whole-cell patch-clamp method leads to a replacement of the intracellular fluid with the intracellular pipet solution. The speed of this replacement or dialysis depends on the cell volume and electrode tip diameter. Although this mechanism can be used advantageously in many experiments, there are conditions where such a ...
openaire   +2 more sources

The Patch Clamp Technique in Ion Channel Research

Current Pharmaceutical Biotechnology, 2004
To understand the pathogenesis of a given ion channel disorder, knowledge of the mutation alone is insufficient, instead, the description of the associated functional defect is decisive. The patch clamp technique enables to achieve this both in native tissue as well as heterologous expression systems. By this technique, structure-function relationships
K, Jurkat-Rott, F, Lehmann-Horn
openaire   +2 more sources

High-Resolution Patch-Clamp Techniques

1985
Ion channels are integral membrane proteins that catalyze the diffusion of ions across cell membranes. For example, a single ion channel may pass 20 pA of current, which is equivalent to a flow of ~108 ions/s. Conformational changes in the channel can turn this flow on and off, thereby producing pulses of current.
Anthony Auerbach, Frederick Sachs
openaire   +1 more source

Patch-Clamp Technique in ESC-Derived Cardiomyocytes

2014
ESC-derived cardiomyocytes are excitable cells that express many of the ion channels also found in adult cardiomyocytes. The patch-clamp technique is a powerful technique to characterize both the electrophysiological properties of excitable cells as well as the underlying ion channel currents responsible for these electrophysiological properties.
Jie, Liu, Peter H, Backx
openaire   +2 more sources

Patch clamp techniques: an overview.

Methods in enzymology, 1992
This chapter provides an overview of the patch clamp techniques. The patch clamp technique provides the experimental means for merging the tools of modern molecular and cellular biology with those of electrophysiology. Using various recording configurations, it is possible to dissect the mechanisms of channel modulation.
M, Cahalan, E, Neher
openaire   +3 more sources

Patch-Clamp Technique in Brain Slices

2003
The technique of patch-clamp recording in brain slices is applicable to a large variety of cell types in slices from nearly all areas of the central nervous system (CNS) in animals at many different stages of development (Blanton et al., 1989; Edwards et al., 1989; Konnerth, 1990).
T. D. Plant, J. Eilers, A. Konnerth
openaire   +1 more source

Patch Clamp Techniques for Plant Cells

2012
The study of electrophysiological processes at the molecular level, with exquisite sensitivity and full control over the experimental conditions, is possible with the patch clamp technique. A concise overview is given of the different configurations that are used with the technique.
openaire   +2 more sources

Patch Clamp Technique and Applications

2017
There are two main modes in patch clamp recordings: voltage clamp and current clamp. In the voltage clamp mode, the membrane voltage is controlled by the amplifier through the recording pipette and the corresponding current through the pipette is measured.
openaire   +1 more source

The patch clamp technique: Principles and technical considerations

Journal of Veterinary Cardiology, 2007
The development of techniques that allow the high fidelity measurement of small scale ionic currents ushered in a new era of investigation into the role of ion channels in the physiologic and pathophysiologic function of excitable tissue. Based upon the formation of a high resistance (gigaohm) seal with the membrane of the cell being studied, these ...
openaire   +2 more sources

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Journal of Visualized Experiments
Endolysosomal ion channels are critical for endolysosomal ion and pH homeostasis, membrane potential regulation, and vesicle trafficking. However, electrophysiologically accessing these channels within small intracellular vesicles has been a challenge.
Hsuan-Ti, Wang, Cheng-Chang, Chen
openaire   +2 more sources