Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
doaj +2 more sources
Scientific Reports, 2021 The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach +9 more
doaj +2 more sources
Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes [PDF]
Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
doaj +2 more sources
Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice [PDF]
Bio-Protocol, 2023 Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess +2 more
doaj +2 more sources
Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology. [PDF]
Int J Mol Sci, 2022 Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such
Linders LE +6 more
europepmc +2 more sources
Frontiers in Physiology, 2018 An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen +7 more
doaj +2 more sources
Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control [PDF]
SensorsThe patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu +5 more
doaj +2 more sources
Communications Biology, 2022 An altered automated patch-clamp (APC) approach enables high-throughput recordings from native pig cardiomyocytes and human iPSC-derived cardiomyocytes.
Fitzwilliam Seibertz +10 more
doaj +2 more sources
The Touch and Zap method for in vivo whole-cell patch recording of intrinsic and visual responses of cortical neurons and glial cells. [PDF]
PLoS ONE, 2014 Whole-cell patch recording is an essential tool for quantitatively establishing the biophysics of brain function, particularly in vivo. This method is of particular interest for studying the functional roles of cortical glial cells in the intact brain ...
Adrien E Schramm +3 more
doaj +7 more sources
Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp [PDF]
BMC Pharmacology and Toxicology, 2015 BACKGROUND: An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology.
Atsushi, Ohtsuki +3 more
core +3 more sources