A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes [PDF]
Communications Biology, 2022 An altered automated patch-clamp (APC) approach enables high-throughput recordings from native pig cardiomyocytes and human iPSC-derived cardiomyocytes.
Fitzwilliam Seibertz +10 more
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Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes [PDF]
Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
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Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
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Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice [PDF]
Bio-Protocol, 2023 Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess +2 more
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Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology. [PDF]
Int J Mol Sci, 2022 Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such
Linders LE +6 more
europepmc +2 more sources
Scientific Reports, 2021 The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach +9 more
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There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments [PDF]
Frontiers in Molecular Neuroscience, 2022 Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius +20 more
doaj +2 more sources
Frontiers in Physiology, 2018 An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen +7 more
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Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts [PDF]
Bio-Protocol, 2021 The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry +5 more
doaj +2 more sources
Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes [PDF]
Frontiers in Physiology, 2023 Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk +2 more
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