Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes [PDF]
Frontiers in Physiology, 2023 Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk +2 more
doaj +4 more sources
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience [PDF]
Frontiers in Neural Circuits, 2015 Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal ...
Can eTao +3 more
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Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes [PDF]
Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
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Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice [PDF]
Bio-Protocol, 2023 Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess +2 more
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Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control [PDF]
SensorsThe patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu +5 more
doaj +2 more sources
A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer [version 2; peer review: 1 approved, 3 approved with reservations] [PDF]
Wellcome Open Research, 2021 Automated patch-clamp platforms are widely used and vital tools in both academia and industry to enable high-throughput studies such as drug screening. A leak current to ground occurs whenever the seal between a pipette and cell (or internal solution and
Chon Lok Lei +7 more
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Protocol to prepare mouse spinal cord for patch-clamp and histology experiments [PDF]
STAR Protocols, 2023 Summary: The spinal cord is a part of the central nervous system located within the spinal canal of the vertebrae. Here, we present a protocol to prepare mouse spinal cord sections for patch-clamp and histology experiments.
Sang-Hyeon Ju, Jong-Woo Sohn
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Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections [PDF]
eLifeSignificant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming.
Mighten C Yip +10 more
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Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices [PDF]
STAR Protocols, 2023 Summary: Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection.
Tori Lhomme, Vincent Prévot
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Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages [PDF]
STAR ProtocolsSummary: Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium
Yingbao Tan +3 more
doaj +2 more sources