Results 11 to 20 of about 752,311 (360)

Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections [PDF]

eLife
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming.
Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest   +10 more
doaj   +2 more sources

Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes [PDF]

Frontiers in Physiology, 2023
Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk, Arie O. Verkerk, Ronald Wilders   +2 more
doaj   +2 more sources

Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience

Frontiers in Neural Circuits, 2015
Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal ...
Can eTao, Guangwei eZhang, Ying eXiong, Yi eZhou   +3 more
doaj   +2 more sources

Early identification of hERG liability in drug discovery programs by automated patch clamp

Frontiers in Pharmacology, 2014
Blockade of the cardiac ion channel coded by hERG can lead to cardiac arrhythmia, which has become a major concern in drug discovery and development.
Timm eDanker, Clemens eMöller
doaj   +2 more sources

Patch clamp-assisted single neuron lipidomics

Scientific Reports, 2017
Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution.
Collin B. Merrill, Abdul Basit, Andrea Armirotti, Yousheng Jia, Christine M. Gall, Gary Lynch, Daniele Piomelli   +6 more
doaj   +2 more sources

Protocol to prepare mouse spinal cord for patch-clamp and histology experiments [PDF]

STAR Protocols, 2023
Summary: The spinal cord is a part of the central nervous system located within the spinal canal of the vertebrae. Here, we present a protocol to prepare mouse spinal cord sections for patch-clamp and histology experiments.
Sang-Hyeon Ju, Jong-Woo Sohn
doaj   +2 more sources

Whole-cell patch-clamp recording and parameters. [PDF]

Biophys Rev, 2023
Kodirov SA.
europepmc   +2 more sources

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +2 more sources

Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices [PDF]

STAR Protocols, 2023
Summary: Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection.
Tori Lhomme, Vincent Prévot
doaj   +2 more sources

Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages [PDF]

STAR Protocols
Summary: Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium
Yingbao Tan, Penghui Chen, Xiaojuan Xin, Jingxue Wang   +3 more
doaj   +2 more sources