Results 11 to 20 of about 152,269 (192)

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +2 more sources

Patch-clamp technique to study mitochondrial membrane biophysics. [PDF]

J Gen Physiol, 2023
Kumari A, Nguyen DM, Garg V.
europepmc   +3 more sources

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi [PDF]

Microsystems & Nanoengineering
Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.
Tanja Pajić, Katarina Stevanović, Nataša V. Todorović, Aleksandar J. Krmpot, Miroslav Živić, Svetlana Savić-Šević, Steva M. Lević, Marina Stanić, Dejan Pantelić, Brana Jelenković, Mihailo D. Rabasović   +10 more
doaj   +2 more sources

There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments

Frontiers in Molecular Neuroscience, 2022
Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius, Alison Obergrussberger, Edward S. A. Humphries, Stephanie Scholz, Ilka Rinke-Weiss, Tom A. Goetze, Nina Brinkwirth, Maria Giustina Rotordam, Tim Strassmaier, Aaron Randolph, Søren Friis, Aiste Liutkute, Aiste Liutkute, Aiste Liutkute, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Niels Voigt, Niels Voigt, Niels Voigt, Niels Fertig   +20 more
doaj   +1 more source

Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts

Bio-Protocol, 2021
The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry, Mighten Yip, Ilya Kolb, William Stoy, Mercedes Gonzalez, Craig Forest   +5 more
doaj   +1 more source

Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps

Sensors, 2023
A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li, Huiying Gong, Jinyu Qiu, Ruimin Li, Qili Zhao, Xin Zhao, Mingzhu Sun   +6 more
doaj   +1 more source

The touch and zap method for in vivo whole-cell patch recording of intrinsic and visual responses of cortical neurons and Glial cells [PDF]

, 2014
Whole-cell patch recording is an essential tool for quantitatively establishing the biophysics of brain function, particularly in vivo. This method is of particular interest for studying the functional roles of cortical glial cells in the intact brain ...
Gener, Thomas, Graham, Lyle, Marinazzo, Daniele, Schramm, Adrien   +3 more
core   +5 more sources

Datasets for calcium dynamics comparison between the whole-cell and a β-escin based perforated patch configuration in brain slices from adult mice

Data in Brief, 2021
Multiple processes shape calcium signals in neurons. The spatial and temporal dynamics of these signals are determined by various cellular parameters, including the calcium influx, calcium buffering, and calcium extrusion.
Simon Hess, Christophe Pouzat, Peter Kloppenburg   +2 more
doaj   +1 more source

Automatic deep learning-driven label-free image-guided patch clamp system

Nature Communications, 2021
Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos, Gáspár Oláh, Tamas Balassa, Norbert Mihut, Márton Rózsa, Attila Ozsvár, Ervin Tasnadi, Pál Barzó, Nóra Faragó, László Puskás, Gábor Molnár, József Molnár, Gábor Tamás, Peter Horvath   +13 more
doaj   +1 more source

Deep learning-based real-time detection of neurons in brain slices for in vitro physiology

Scientific Reports, 2021
A common electrophysiology technique used in neuroscience is patch clamp: a method in which a glass pipette electrode facilitates single cell electrical recordings from neurons.
Mighten C. Yip, Mercedes M. Gonzalez, Christopher R. Valenta, Matthew J. M. Rowan, Craig R. Forest   +4 more
doaj   +1 more source