Results 21 to 30 of about 152,269 (192)
Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices
STAR Protocols, 2021 Summary: Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current ...
Kevin M. Manz +3 more
doaj +1 more source
Stem Cell Research, 2019 Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology.
Wener Li +6 more
doaj +1 more source
Protocol to study microcircuits in the medial entorhinal cortex in mice using multiple patch-clamp recordings and morphological reconstruction [PDF]
STAR ProtocShi Y, Wang G.
europepmc +2 more sources
Early identification of hERG liability in drug discovery programs by automated patch clamp
Frontiers in Pharmacology, 2014 Blockade of the cardiac ion channel coded by hERG can lead to cardiac arrhythmia, which has become a major concern in drug discovery and development.
Timm eDanker, Clemens eMöller
doaj +1 more source
Batch Fabrication of High-Performance Planar Patch-Clamp Devices in Quartz [PDF]
, 2010 The success of the patch-clamp technique has driven an effort to create wafer-based patch-clamp platforms. We develop a lithographic/electrochemical processing scheme that generates ultrasmooth, high aspect ratio pores in quartz.
Heath, James R. +5 more
core +1 more source
PLoS ONE, 2021 Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan +3 more
doaj +1 more source
Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp [PDF]
, 2015 BACKGROUND: An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology.
Atsushi, Ohtsuki +3 more
core +2 more sources
Bioengineering, 2020 Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C.
Jann Harberts +4 more
doaj +1 more source
Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization
eLife, 2021 The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons.
Brian R Lee +27 more
doaj +1 more source
Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels [PDF]
, 2013 Important modulatory roles have been attributed to presynaptic NMDA receptors (NMDARs) located on cerebellar interneuron terminals. Evidence supporting a presynaptic location includes an increase in the frequency of mini events following the application ...
Benton, DCH +4 more
core +1 more source