Results 21 to 30 of about 752,311 (360)
In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi [PDF]
Microsystems & NanoengineeringStudying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.
Tanja Pajić +10 more
doaj +2 more sources
Frontiers in Molecular Neuroscience, 2022 Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius +20 more
doaj +1 more source
Bio-Protocol, 2021 The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry +5 more
doaj +1 more source
Analytical Chemistry, 2021 Understanding of the relationship between cellular function and molecular composition holds a key to next-generation therapeutics but requires measurement of all types of molecules in cells.
Sam B. Choi, Abigail M. Polter, P. Nemes
semanticscholar +1 more source
In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives
Italian National Conference on Sensors, 2021 Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp ...
Asako Noguchi, Y. Ikegaya, N. Matsumoto
semanticscholar +1 more source
Biophysical Journal, 2002 To use one of the new benchtop patch-clamp systems, you first harvest the cells from a tissue-culture dish and wash by pelleting them in a microcentrifuge. Then you open the lid of the patch clamp box and pipette a few microliters of the cell suspension into the recording well. After a few minutes the cells settle onto the electrode array at the bottom
Kathryn G. Klemic, Fred J. Sigworth
openaire +3 more sources
Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps
Sensors, 2023 A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li +6 more
doaj +1 more source
Deep learning-based real-time detection of neurons in brain slices for in vitro physiology
Scientific Reports, 2021 A common electrophysiology technique used in neuroscience is patch clamp: a method in which a glass pipette electrode facilitates single cell electrical recordings from neurons.
Mighten C. Yip +4 more
doaj +1 more source
Whole-GUV patch-clamping [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems.
Lars D. Mosgaard +9 more
openaire +4 more sources
Automated patch clamp in drug discovery: major breakthroughs and innovation in the last decade
Expert Opinion on Drug Discovery, 2020 Patch-clamp electrophysiology remains an important technique in studying ion channels; indeed, it is still considered the gold standard since it was first described by Neher and Sakmann in the 1970...
Alison Obergrussberger +3 more
semanticscholar +1 more source