Results 31 to 40 of about 752,311 (360)

Deep-Channel uses deep neural networks to detect single-molecule events from patch-clamp data

Communications Biology, 2020
Single-molecule research techniques such as patch-clamp electrophysiology deliver unique biological insight by capturing the movement of individual proteins in real time, unobscured by whole-cell ensemble averaging. The critical first step in analysis is
Numan Celik, Fiona O’Brien, S. Brennan, R. D. Rainbow, C. Dart, Yalin Zheng, Frans Coenen, R. Barrett-Jolley   +7 more
semanticscholar   +1 more source

A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer [version 2; peer review: 1 approved, 3 approved with reservations]

Wellcome Open Research, 2021
Automated patch-clamp platforms are widely used and vital tools in both academia and industry to enable high-throughput studies such as drug screening. A leak current to ground occurs whenever the seal between a pipette and cell (or internal solution and
Chon Lok Lei, Alan Fabbri, Dominic G. Whittaker, Michael Clerx, Monique J. Windley, Adam P. Hill, Gary R. Mirams, Teun P. de Boer   +7 more
doaj   +1 more source

Transfection methods for high-throughput cellular assays of voltage-gated calcium and sodium channels involved in pain.

PLoS ONE, 2021
Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan, Lotten Ragnarsson, Fernanda C Cardoso, Richard J Lewis   +3 more
doaj   +1 more source

Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization

eLife, 2021
The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons.
Brian R Lee, Agata Budzillo, Kristen Hadley, Jeremy A Miller, Tim Jarsky, Katherine Baker, DiJon Hill, Lisa Kim, Rusty Mann, Lindsay Ng, Aaron Oldre, Ram Rajanbabu, Jessica Trinh, Sara Vargas, Thomas Braun, Rachel A Dalley, Nathan W Gouwens, Brian E Kalmbach, Tae Kyung Kim, Kimberly A Smith, Gilberto Soler-Llavina, Staci Sorensen, Bosiljka Tasic, Jonathan T Ting, Ed Lein, Hongkui Zeng, Gabe J Murphy, Jim Berg   +27 more
doaj   +1 more source

Batch Fabrication of High-Performance Planar Patch-Clamp Devices in Quartz [PDF]

, 2010
The success of the patch-clamp technique has driven an effort to create wafer-based patch-clamp platforms. We develop a lithographic/electrochemical processing scheme that generates ultrasmooth, high aspect ratio pores in quartz.
Heath, James R., Hwang, Gyeong S., Luo, Yi, Nagarah, John M., Paek, Eunsu, Wang, Pin   +5 more
core   +1 more source

Sperm Patch-Clamp [PDF]

, 2013
Sperm intracellular pH and calcium concentration ([Ca(2+)]i) are two central factors that control sperm activity within the female reproductive tract. As such, the ion channels of the sperm plasma membrane that alter intracellular sperm [Ca(2+)] and pH play important roles in sperm physiology and the process of fertilization. Indeed, sperm ion channels
Betsy Navarro, Betsy Navarro, Polina V. Lishko, Yuriy Kirichok, David E. Clapham, David E. Clapham   +5 more
openaire   +3 more sources

Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology [PDF]

, 2017
Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed “blind,” meaning that throughput for ...
Alexander R. Morris, Andrásfalvy, Blanton, Caroline S. Copeland, Cheng, Covey, Denk, DeWeese, Dittgen, Edwards, Ferster, Fertig, Golshani, Gonzalez, Hamill, Isard, Kitamura, Kodandaramaiah, Komai, Long, Luca A. Annecchino, Manzo, Margrie, Margrie, Nimmerjahn, Oshiorenoya E. Agabi, Otsu, Pak, Paul Chadderton, Pei, Rancz, Sakmann, Schultz, Simon R. Schultz, Stosiek, Stuart, Tamamaki   +36 more
core   +4 more sources

A nongenomic mechanism for progesterone-mediated immunosuppression: Inhibition of K+ channels, Ca2+ signaling, and gene expression in T lymphocytes [PDF]

, 1998
The mechanism by which progesterone causes localized suppression of the immune response during pregnancy has remained elusive. Using human T lymphocytes and T cell lines, we show that progesterone, at concentrations found in the placenta, rapidly and ...
Beer, Bergeron, Bertrand, Bielefeldt, Blackmore, Breit, Cahalan, Cahalan, Chandy, Clemens, Crabtree, Dolmetsch, Fiering, Foresta, Gouy, Grissmer, Grynkiewicz, Gurka, Hamill, Hess, Horn, Hunt, Karttunen, Kerschbaum, Koo, Lewis, Lewis, Lin, Logsdon, Mason, Michne, Monterroso, Negulescu, Nguyen, Pavia, Pepe, Phipps, Premack, Price, Revelli, Roussev, Runnebaum, Runnebaum, Shastri, Siiteri, Siiteri, Stites, Thastrup, Timmerman, Valera, Van Voorhis, Verheugen, Waldegger, Wu, Zweifach   +54 more
core   +3 more sources

A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons

Bioengineering, 2020
Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C.
Jann Harberts, Max Kusch, John O’Sullivan, Robert Zierold, Robert H. Blick   +4 more
doaj   +1 more source

Preparation of Giant Escherichia coli spheroplasts for Electrophysiological Recordings

Bio-Protocol, 2021
Prokaryotic ion channels have been instrumental in furthering our understanding of many fundamental aspects of ion channels’ structure and function. However, characterizing the biophysical properties of a prokaryotic ion channel in a native membrane ...
Yihao Jiang, Vinay Idikuda, Baron Chanda
doaj   +1 more source