Results 11 to 20 of about 51,351 (304)

Generating Patient-Derived Gliomas within Cerebral Organoids

STAR Protocols, 2020
Summary: Glioblastoma (GBM) remains a devastating disease with a median survival of less than two years. Current preclinical models are unable to accurately reflect the complexity of human GBM.
Amanda Linkous, Howard A. Fine
doaj   +3 more sources

Patient-derived spheroids and patient-derived organoids simulate evolutions of lung cancer

Heliyon, 2023
Cancer cells harbor many genetic mutations and gene expression profiles different from normal cells. Patient-derived cancer cells (PDCC) are preferred materials in cancer study.
Surina, Tanggis, Tomoko Suzuki, Shu Hisata, Kazutaka Fujita, Satomi Fujiwara, Fangyuan Liu, Noriyoshi Fukushima, Takuji Suzuki, Naoko Mato, Koichi Hagiwara   +10 more
doaj   +3 more sources

Modeling Patient-Derived Glioblastoma with Cerebral Organoids [PDF]

Cell Reports, 2019
Summary: The prognosis of patients with glioblastoma (GBM) remains dismal, with a median survival of approximately 15 months. Current preclinical GBM models are limited by the lack of a “normal” human microenvironment and the inability of many tumor cell
Amanda Linkous, Demosthenes Balamatsias, Matija Snuderl, Lincoln Edwards, Ken Miyaguchi, Teresa Milner, Batsheva Reich, Leona Cohen-Gould, Andrew Storaska, Yasumi Nakayama, Emily Schenkein, Richa Singhania, Stefano Cirigliano, Tarig Magdeldin, Ying Lin, Gouri Nanjangud, Kalyani Chadalavada, David Pisapia, Conor Liston, Howard A. Fine   +19 more
doaj   +3 more sources

Generation and characterization of NGLY1 patient-derived midbrain organoids

Frontiers in Cell and Developmental Biology, 2023
NGLY1 deficiency is an ultra-rare, autosomal recessive genetic disease caused by mutations in the NGLY1 gene encoding N-glycanase one that removes N-linked glycan.
Joshua Abbott, Mitali Tambe, Ivan Pavlinov, Atena Farkhondeh, Ha Nam Nguyen, Ha Nam Nguyen, Miao Xu, Manisha Pradhan, Tate York, Matthew Might, Karsten Baumgärtel, Steven Rodems, Wei Zheng   +12 more
doaj   +3 more sources

Patient-Derived Organoids of Cholangiocarcinoma [PDF]

International Journal of Molecular Sciences, 2021
Cholangiocarcinoma (CC) is an aggressive malignancy with an inferior prognosis due to limited systemic treatment options. As preclinical models such as CC cell lines are extremely rare, this manuscript reports a protocol of cholangiocarcinoma patient-derived organoid culture as well as a protocol for the transition of 3D organoid lines to 2D cell lines.
Christopher Fabian Maier, Lei Zhu, Lahiri Kanth Nanduri, Daniel Kühn, Susan Kochall, May-Linn Thepkaysone, Doreen William, Konrad Grützmann, Barbara Klink, Johannes Betge, Jürgen Weitz, Nuh N. Rahbari, Christoph Reißfelder, Sebastian Schölch   +13 more
openaire   +2 more sources

Patient-Derived Organoids for Precision Cancer Immunotherapy [PDF]

Cancer Research, 2021
Abstract Cancer immunotherapy has revolutionized the way tumors are treated. Nevertheless, efficient and robust testing platforms are still missing, including clinically relevant human ex vivo tumor assays that allow pretreatment testing of cancer therapies and selection of the most efficient and safe therapy for a specific patient.
Mikaela Grönholm, Michaela Feodoroff, Gabriella Antignani, Beatriz Martins, Firas Hamdan, Vincenzo Cerullo   +5 more
openaire   +3 more sources

Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids [PDF]

Cells, 2020
Cancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (CCA) PDO is still sparse but growing.
Amato, Francesco, Rae, Colin, Prete, Maria Giuseppin, Braconi, Chiara   +3 more
openaire   +4 more sources

Patient-Derived Organoid Models of Human Neuroendocrine Carcinoma [PDF]

Frontiers in Endocrinology, 2021
Gastroenteropancreatic neuroendocrine carcinoma (GEP-NEC) is a poorly understood disease with limited treatment options. A better understanding of this disease would greatly benefit from the availability of representative preclinical models. Here, we present the potential of tumor organoids, three-dimensional cultures of tumor cells, to model GEP-NEC ...
Krijn K. Dijkstra, Krijn K. Dijkstra, José G. van den Berg, Fleur Weeber, Fleur Weeber, Joris van de Haar, Joris van de Haar, Arno Velds, Sovann Kaing, Sovann Kaing, Dennis D. G. C. Peters, Ferry A. L. M. Eskens, Derk-Jan A. de Groot, Margot E. T. Tesselaar, Emile E. Voest, Emile E. Voest   +15 more
openaire   +5 more sources

Ultrastructural analysis of breast cancer patient-derived organoids [PDF]

Cancer Cell International, 2021
Abstract Background Breast cancer Patient Derived Organoids (PDO) have been demonstrated to be a reliable model to study cancer that promised to replace and reduce the use of animals in pre-clinical research. They displayed concordance with the tissue of origin, resuming its heterogenicity and representing a good ...
Signati L., Allevi R., Piccotti F., Albasini S., Villani L., Sevieri M., Bonizzi A., Corsi F., Mazzucchelli S.   +8 more
openaire   +4 more sources

Patient-Derived Cancer Organoids for Precision Oncology Treatment [PDF]

Journal of Personalized Medicine, 2021
The emergence of three-dimensional human organoids has opened the door for development of patient-derived cancer organoid (PDO) models, which closely recapitulate parental tumor tissue. Mainstays of preclinical cancer modeling include in vitro cell lines and patient-derived xenografts, but these models lack the cellular heterogeneity seen in human ...
Mark N. Pernik, Cylaina E. Bird, Jeffrey I. Traylor, Diana D. Shi, Timothy E. Richardson, Samuel K. McBrayer, Kalil G. Abdullah   +6 more
openaire   +2 more sources