Results 21 to 30 of about 38,270 (217)

Generating Patient-Derived Gliomas within Cerebral Organoids

STAR Protocols, 2020
Summary: Glioblastoma (GBM) remains a devastating disease with a median survival of less than two years. Current preclinical models are unable to accurately reflect the complexity of human GBM.
Amanda Linkous, Howard A. Fine
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

3D brain Organoids derived from pluripotent stem cells: promising experimental models for brain development and neurodegenerative disorders

Journal of Biomedical Science, 2017
Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), appear to recapitulate the brain’s 3D cytoarchitectural arrangement and provide ...
Chun-Ting Lee, Raphael M. Bendriem, Wells W. Wu, Rong-Fong Shen   +3 more
doaj   +1 more source

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

iScience, 2020
Summary: Lung cancer is an extremely heterogeneous disease, and its treatment remains one of the most challenging tasks in medicine. Few existing laboratory lung cancer models can faithfully recapitulate the diversity of the disease and predict therapy ...
Zhichao Li, Youhui Qian, Wujiao Li, Lisa Liu, Lei Yu, Xia Liu, Guodong Wu, Youyu Wang, Weibin Luo, Fuyuan Fang, Yuchen Liu, Fei Song, Zhiming Cai, Wei Chen, Weiren Huang   +14 more
doaj   +1 more source

Application of human liver organoids as a patient-derived primary model for HBV infection and related hepatocellular carcinoma

eLife, 2021
The molecular events that drive hepatitis B virus (HBV)-mediated transformation and tumorigenesis have remained largely unclear, due to the absence of a relevant primary model system.
Elisa De Crignis, Tanvir Hossain, Shahla Romal, Fabrizia Carofiglio, Panagiotis Moulos, Mir Mubashir Khalid, Shringar Rao, Ameneh Bazrafshan, Monique MA Verstegen, Farzin Pourfarzad, Christina Koutsothanassis, Helmuth Gehart, Tsung Wai Kan, Robert-Jan Palstra, Charles Boucher, Jan NM IJzermans, Meritxell Huch, Sylvia F Boj, Robert Vries, Hans Clevers, Luc JW van der Laan, Pantelis Hatzis, Tokameh Mahmoudi   +22 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Metabolic Heterogeneity in Patient Tumor-Derived Organoids by Primary Site and Drug Treatment

Frontiers in Oncology, 2020
New tools are needed to match cancer patients with effective treatments. Patient-derived organoids offer a high-throughput platform to personalize treatments and discover novel therapies.
Joe T. Sharick, Joe T. Sharick, Christine M. Walsh, Carley M. Sprackling, Cheri A. Pasch, Dan L. Pham, Dan L. Pham, Karla Esbona, Alka Choudhary, Alka Choudhary, Rebeca Garcia-Valera, Rebeca Garcia-Valera, Rebeca Garcia-Valera, Mark E. Burkard, Mark E. Burkard, Stephanie M. McGregor, Stephanie M. McGregor, Kristina A. Matkowskyj, Kristina A. Matkowskyj, Kristina A. Matkowskyj, Alexander A. Parikh, Ingrid M. Meszoely, Mark C. Kelley, Susan Tsai, Dustin A. Deming, Dustin A. Deming, Dustin A. Deming, Melissa C. Skala, Melissa C. Skala, Melissa C. Skala   +29 more
doaj   +1 more source

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis

Genome Biology, 2022
Background Patient-derived organoid culture is a powerful system for studying the molecular mechanisms of cancers, especially colorectal cancer (CRC), one of the most prevalent cancers worldwide. There are two main types of 3D culture methods for colonic
Rui Wang, Yunuo Mao, Wendong Wang, Xin Zhou, Wei Wang, Shuai Gao, Jingyun Li, Lu Wen, Wei Fu, Fuchou Tang   +9 more
doaj   +1 more source