Results 21 to 30 of about 51,351 (304)

Patient-Derived Organoids in Precision Medicine: Drug Screening, Organoid-on-a-Chip and Living Organoid Biobank [PDF]

Frontiers in Oncology, 2021
Organoids are in vitro self-assembling, organ-like, three-dimensional cellular structures that stably retain key characteristics of the respective organs. Organoids can be generated from healthy or pathological tissues derived from patients. Cancer organoid culture platforms have several advantages, including conservation of the cellular composition ...
Zilong Zhou, Lele Cong, Xianling Cong
openaire   +3 more sources

Modeling retinitis pigmentosa through patient-derived retinal organoids

STAR Protocols, 2021
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene ...
Yan-Ping Li, Wen-Li Deng, Zi-Bing Jin
openaire   +3 more sources

Advances of Patient-Derived Organoids in Personalized Radiotherapy

Frontiers in Oncology, 2022
Patient-derived organoids (PDO), based on the advanced three-dimensional (3D) culture technology, can provide more relevant physiological and pathological cancer models, which is especially beneficial for developing and optimizing cancer therapeutic strategies. Radiotherapy (RT) is a cornerstone of curative and palliative cancer treatment, which can be
Yuenan Wang, Ye Li, Zonghai Sheng, Weiwei Deng, Hongyan Yuan, Shubin Wang, Yajie Liu   +6 more
openaire   +3 more sources

Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids [PDF]

iScience, 2020
Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings.
Anna E. Vilgelm, Kensey Bergdorf, Melissa Wolf, Vijaya Bharti, Rebecca Shattuck-Brandt, Ashlyn Blevins, Caroline Jones, Courtney Phifer, Mason Lee, Cindy Lowe, Rachel Hongo, Kelli Boyd, James Netterville, Sarah Rohde, Kamran Idrees, Joshua A. Bauer, David Westover, Bradley Reinfeld, Naira Baregamian, Ann Richmond, W. Kimryn Rathmell, Ethan Lee, Oliver G. McDonald, Vivian L. Weiss   +23 more
openaire   +3 more sources

Patient-Derived Cancer Organoids as Predictors of Treatment Response [PDF]

Frontiers in Oncology, 2021
Patient-derived cancer organoids have taken a prominent role in pre-clinical and translational research and have been generated for most common solid tumors. Cancer organoids have been shown to retain key genetic and phenotypic characteristics of their tissue of origin, tumor subtype and maintain intratumoral heterogeneity and therefore have the ...
Verduin, M., Hoeben, A., De Ruysscher, D., Vooijs, M.   +3 more
openaire   +4 more sources

Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent [PDF]

, 2020
Induced pluripotent stem cell (iPSC)‐derived retinal organoids provide a platform to study human retinogenesis, disease modeling, and compound screening.
Armstrong, Lyle, Carter, Madeleine, Chichagova, Valeria, Georgiou, Maria, Ghareeb, Ali, Hilgen, Gerrit, Lako, Majlinda, Sernagor, Evelyne   +7 more
core   +2 more sources

Application Progress of Organoids in Colorectal Cancer

Frontiers in Cell and Developmental Biology, 2022
Currently, colorectal cancer is still the third leading cause of cancer-related mortality, and the incidence is rising. It is a long time since the researchers used cancer cell lines and animals as the study subject. However, these models possess various
Lianxiang Luo, Lianxiang Luo, Lianxiang Luo, Yucui Ma, Yilin Zheng, Jiating Su, Guoxin Huang   +6 more
doaj   +1 more source

An Organoid-Based Preclinical Model of Human Gastric CancerSummary

Cellular and Molecular Gastroenterology and Hepatology, 2019
Background & Aims: Our goal was to develop an initial study for the proof of concept whereby gastric cancer organoids are used as an approach to predict the tumor response in individual patients.
Nina G. Steele, Jayati Chakrabarti, Jiang Wang, Jacek Biesiada, Loryn Holokai, Julie Chang, Lauren M. Nowacki, Jennifer Hawkins, Maxime Mahe, Nambirajan Sundaram, Noah Shroyer, Mario Medvedovic, Michael Helmrath, Syed Ahmad, Yana Zavros   +14 more
doaj   +1 more source

Large variety in a panel of human colon cancer organoids in response to EZH2 inhibition [PDF]

, 2016
EZH2 inhibitors have gained great interest for their use as anti-cancer therapeutics. However, most research has focused on EZH2 mutant cancers and recently adverse effects of EZH2 inactivation have come to light.
Bounova, Gergana, Cornelissen-Steijger, Paulien, de Vries, Nienke, Koppens, Martijn A.J., Sansom, Owen J., van Lohuizen, Maarten, Wessels, Lodewyk F.A.   +6 more
core   +1 more source

Semi-Automated Computational Assessment of Cancer Organoid Viability Using Rapid Live-Cell Microscopy

Cancer Informatics, 2022
The creation of patient-derived cancer organoids represents a key advance in preclinical modeling and has recently been applied to a variety of human solid tumor types.
Joseph D Buehler, Cylaina E Bird, Milan R Savani, Lauren C Gattie, William H Hicks, Michael M Levitt, Mohamad El Shami, Kimmo J Hatanpaa, Timothy E Richardson, Samuel K McBrayer, Kalil G Abdullah   +10 more
doaj   +1 more source