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Mycolicibacterium mageritense Bacteremia Following Partial Nephrectomy: A Case Report. [PDF]

Cureus
Castro R.
europepmc   +1 more source

Denaturing Gradient Gel Electrophoresis Approach for Microbial Shift Analysis in Thermophilic and Mesophilic Anaerobic Digestions. [PDF]

Gels
Pandey P, Chowdhury D, Wang Y.
europepmc   +1 more source

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Effect of DNA polymerases on PCR-DGGE patterns

International Biodeterioration and Biodegradation, 2013
Abstract PCR-based molecular biological techniques became fundamental in the analysis of microbial communities. One of the most popular techniques is PCR-DGGE wherein the outcome is greatly influenced by the DNA amplification process. A large number of different factors were described as key elements for successful amplification, however the ...
Margit Balázs, Andrea Rónavári, Alexandra Nemeth   +2 more
exaly   +2 more sources

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

Journal of Microbiological Methods, 2004
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed:
Danilo Ercolini
exaly   +4 more sources

Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis

International Journal of Food Microbiology, 2003
The use of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique in identifying the microorganisms present in commercial probiotic yoghurts and lyophilised products was evaluated. Two reference ladders were assembled constituted by PCR-amplified V2-V3 regions of 16S rDNA from bacterial species generally used as ...
Franca Rossi, Franco Dellaglio, Sandra Torriani   +2 more
exaly   +3 more sources

Rapid identification of Armillaria species by PCR–DGGE

Journal of Microbiological Methods, 2014
The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.
Longa, Claudia Maria Oliveira, La Porta, Nicola   +1 more
openaire   +3 more sources

PCR–DGGE differentiation of strains of Saccharomyces sensu stricto

Antonie van Leeuwenhoek, 2004
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S.
Marisa, Manzano, Luca, Cocolin, Benedetta, Longo, Giuseppe, Comi   +3 more
openaire   +2 more sources

Use of the PCR-DGGE method in forensic microbiology

Issues of Forensic Science, 2016
The latest reports on the subject of forensic microbiology indicate that it is possible to link a perpetrator of an offence to the evidence, based on molecular analysis of microbiological material. The microbiome of human epidermis is known to be species- and individual-specific.
Aleksandra Ziembińska-Buczyńska, Krzysztof Kraśnicki   +1 more
openaire   +1 more source

Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE

Current Microbiology, 2009
Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens.
Akihito, Endo, Y, Futagawa-Endo, L M T, Dicks   +2 more
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PCR-DGGE analysis of bacterial population attached to the bovine rumen wall

Folia Microbiologica, 2010
We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken ...
Lukáš, F. (Filip), Šimůnek, J. (Jiří), Mrázek, J. (Jakub), Kopečný, J. (Jan)   +3 more
openaire   +3 more sources