Results 171 to 180 of about 861,394 (224)

Differentiation of Sturgeon Species by PCR-RFLP

Food Research International, 1999
Abstract A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction ...
Philipp Hübner, Jürg Lüthy
exaly   +2 more sources

PCR-RFLP for Aspergillus Species

2016
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is the most simple method for single-nucleotide change detection. It is widely used in the detection and differentiation between mycotoxigenic species. It is based on PCR amplification of a target region containing the variant site of the studied species followed by ...
Ali, Atoui, André, El Khoury
openaire   +2 more sources

Identification of clinically relevant yeasts by PCR/RFLP

Journal of Microbiological Methods, 2004
For molecular diagnosis of fungal disease using DNA amplification procedures in the routine laboratory, choice of appropriate target structures and rapid and inexpensive identification of amplification products are important prerequisites. Most diagnostic procedures described thus far are characterized by limited applicability, considerable cost for ...
Anja, Trost, Barbara, Graf, Jan, Eucker, Orhan, Sezer, Kurt, Possinger, Ulf B, Göbel, Thomas, Adam   +6 more
openaire   +2 more sources

Comprehensive typing of DQB1 alleles by PCR‐RFLP

Tissue Antigens, 1994
Abstract: The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group‐specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively.
D P, Sengar, R, Goldstein
openaire   +2 more sources

Discrimination of Penaeid Shrimps with PCR-RFLP Analysis

Journal of Shellfish Research, 2008
Abstract A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was designed to examine genetic differentiation of some penaeid shrimps. Three hundred and fifty-six bp of cytochrome-b gene, a specific part of mitochondrial genome, was amplified with PCR to figure out differences between shrimp species Penaeus ...
HİSAR, OLCAY, Hisar, Sukriye Aras, Mol, Suhendan, AKSAKAL, ERCÜMENT, YANIK, TELAT   +4 more
openaire   +3 more sources

Identification of common species of dermatophytes by PCR-RFLP

Journal of Huazhong University of Science and Technology [Medical Sciences], 2005
To establish a simple, sensitive and effective technique for the identification of six common dermatophytes, polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) targeting Topoisomerase II gene were used. The DNA of 6 common dermatophytes was amplified by primer dPsD1 and then primers dPsD2.
Ganlin, He, Jiawen, Li, Juan, Ding, Zhijan, Tan   +3 more
openaire   +2 more sources

Automated, PCR-RFLP Genotyping of the Urokinase Gene

Genetic Testing, 2000
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et aL, 1998). Recently, a common genetic polymorphism in the untranslated region of the u-PA gene was shown to be associated with syptomatic CAD.
S M, Sell, F, Blasi, F, Booyse
openaire   +2 more sources

ABO genotyping by PCR-RFLP and cloning and sequencing

Anthropologischer Anzeiger, 2004
A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications.
Haak, W., Burger, J., Alt, K.
openaire   +4 more sources