Results 191 to 200 of about 655,509 (235)

ABO genotyping by PCR-RFLP and cloning and sequencing

Anthropologischer Anzeiger, 2004
A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications.
Haak, W., Burger, J., Alt, K.
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Molecular characterization of potato virus YN isolates by PCR-RFLP

European Journal of Plant Pathology, 1996
Based on the sequence polymorphism in the 5′ terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5′ non-translated and P1 protein region were tested.
Glais, L., Kerlan, Camille, Tribodet, Michel, Marie-Jeanne, Véronique, Robaglia, Christophe, Astier-Manifacier, S.   +5 more
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A PstI PCR-RFLP at the goat CSN1S2 gene.

Animal genetics, 1999
By means of PCR the DNA region spanning from the 17th to the 18th exon of the goat CSN1S2 gene was amplified. PCR products were digested with PstI endonuclease. Digestion products showed a triallelic polymorphism (A, B and C). The frequencies of A, B and C alleles in 155 goats of undefined type reared in Southern Italy were 0.31, 0.40 and 0.29 ...
RAMUNNO, LUIGI, LONGOBARDI E., COSENZA, GIANFRANCO, DI GREGORIO P., RANDO A., MASINA P.   +5 more
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Diagnosis of Penicillin Resistance by PCR-RFLP

2003
Streptococcus pneumoniae is an important human pathogen causing a wide spectrum of disease including pneumonia, otitis media, bacteraemia, and meningitis. It is a significant cause of morbidity and mortality worldwide and now penicillin resistance is becoming an ever increasing problem (1-2). Initially, all S.
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PCR, RFLP

2003
Tehnika umnažanja DNA, lančana reakcija polimerazom (PCR), revolucionarna je metoda današnjice. Metoda omogućava eksponencijalno umnažanje ciljnog dijela DNA u uvjetima in vitro. Pokazala se najspecifičnijom i najosjetljivijom za molekularno-genetičke analize. Postoje različiti pristupi samog umnažanja: 1. jednostruki PCR, 2. višestruki PCR, 3.
Grce, Magdalena, Matovina, Mihaela, Husnjak, Koraljka, Milutin, Nina   +3 more
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A rapid PCR–RFLP method for the identification of Lophius species

European Food Research and Technology, 2012
The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction–restriction fragment length polymorphism ...
ARMANI, ANDREA, CASTIGLIEGO, LORENZO, TINACCI, LARA, Gandini G, GIANFALDONI, DANIELA, GUIDI, ALESSANDRA   +5 more
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An Improved Natural PCR-RFLP Primer Design Method

2011 IEEE 11th International Conference on Bioinformatics and Bioengineering, 2011
The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique is often used in laboratories and many basic research studies of complex genetic diseases associated with single nucleotide polymorphisms (SNP). When performing PCR-RFLP for SNP genotyping, feasible primer pairs are subject to numerous constraints and require a ...
Li-Yeh Chuang, Yu-Da Lin, Hsueh-Wei Chang, Cheng-Hong Yang 0001   +3 more
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Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

Folia Microbiologica, 2006
The suitability of restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orpinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments--nuclear small subunit (SSU; 18S rDNA), the nuclear large subunit (LSU; 28S rDNA) and internal transcribed ...
K, Fliegerová, J, Mrázek, K, Voigt
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การตรวจวินิจฉัยแยกชนิดเชื้อบิดในไก่ด้วยวิธี PCR-RFLP

โรคบิดในไก่เป็นโรคโปรโตซัวมีสาเหตุมาจากเชื้อบิดในสกุล Eimeria โดยทั่วไปพื้นฐานของการตรวจแยกชนิดเชื้อบิดในไก่เป็นการแยกจากลักษณะรูปร่างของเชื้อบิดและบริเวณตำแหน่งในลำไส้ของไก่ที่ติดเชื้อบิด ซึ่งจากหลักการนี้พบว่าการตรวจแยกความแตกต่างชนิดของเชื้อบิดในบางครั้งให้ผลไม่น่าเชื่อถือ จึงได้มีการพัฒนานำวิธี Polymerase Chain Reaction-Restriction Fragment Length ...
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Differentiation of H. pylori Strains Using PCR RFLP

2003
Helicobacter pylori strains have been shown to display considerable heterogeneity with respect to DNA sequence. Diverse restriction fragment length polymorphism (RFLP) patterns are generated among strains by restriction endonuclease digestion of whole chromosomal DNA (1-3), digestion of specific polymerase chain reaction (PCR) products (4), or ...
H L, Mobley, P A, Foxall
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