Diagnosis of quinolone-resistant Coxiella burnetii strains by PCR-RFLP [PDF]
Journal of Clinical Laboratory Analysis, 2000 A total of 12 strains of Coxiella burnetii (8 Greek isolates from acute Q-fever patients, two reference strains-Nine Mile and Q212-and two pefloxacin-resistant laboratory strains) were examined for the presence of point mutations in the quinolone resistance determining region (QRDR) of gyrA gene by direct DNA sequencing of the polymerase chain reaction
Ioanna Spyridaki +4 more
openalex +4 more sources
Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Brazilian Journal of Infectious Diseases, 2011 Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc +4 more
doaj +2 more sources
Differentiation of Brucella abortus and B. melitensis biovars using PCR-RFLP and REP-PCR [PDF]
New Microbes and New Infections, 2019 Brucellosis is one of the most common zoonotic diseases of animal and human beings. This study aimed to differentiate the Brucella spp. and determines the patterns of biovars by using repetitive element palindromic (REP)-PCR and PCR restriction fragment ...
M. Amoupour +6 more
doaj +2 more sources
PCR-RFLP Genotyping of Murine MHC Haplotypes
BioTechniques, 1996 Stanford L. Peng, Joseph Craft
doaj +4 more sources
Molecular Typing of Methicillin Resistant Staphylococcus aureus using coa Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism: A Cross-sectional Study [PDF]
Journal of Clinical and Diagnostic Research, 2022 Introduction: Methicillin Resistant Staphylococcus aureus (MRSA) are the most important multidrug resistant pathogen of humans causing a wide array of infections.
P Hema, B Appalaraju, R Someshwaran
doaj +1 more source
Differentiation between Aspergillus flavus and Aspergillus parasiticus from pure culture and aflatoxin-contaminated grapes using PCR-RFLP analysis of aflR-aflJ intergenic spacer. [PDF]
Journal of Food Science, 2011 Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
A. El Khoury +5 more
semanticscholar +3 more sources
Identification of Eutypa lata by PCR-RFLP [PDF]
Plant Disease, 2004 Eutypa lata is a vascular canker pathogen of woody plants commonly diagnosed by isolating the pathogen from infected tissue. Related fungi from the same family, the Diatrypaceae, also have been found in association with grapevine in Californian vineyards. An in situ polymerase chain reaction (PCR) method has been developed for detection of E.
Walter D. Gubler +2 more
openaire +2 more sources
تمرکز ایزوالکتریک و روشهای متصل به PCR-RFLP برای کشف کمبود Alpha1-antitrypsin [PDF]
Archives of Razi Institute, 2004 53 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF ...
ب.غ. گودرزی +4 more
doaj +1 more source
FEATURES OF DIAGNOSIS OF NECROBACTERIOSIS OF COWS BY PCR-RFLP
Podilian Bulletin Agriculture Engineering Economics, 2023 Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established.
Biriukova, Olha +4 more
openaire +4 more sources
Characterization of Ascaris from Ecuador and Zanzibar [PDF]
, 2014 To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (
A.M. Sparks +9 more
core +5 more sources