Results 141 to 150 of about 4,982,154 (391)

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Frontiers in Microbiology, 2017
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.
P. Kralik, M. Ricchi
semanticscholar   +1 more source

Patient‐specific pharmacogenomics demonstrates xCT as predictive therapeutic target in colon cancer with possible implications in tumor connectivity

Molecular Oncology, EarlyView.
This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker, Keren Zohar, Martin Böttcher, Thomas Wartmann, Henry Freudenstein, Maximilian Doelling, Mihailo Andric, Wenjie Shi, Or Kakhlon, Katrin Hippe, Beatrix Jahnke, Dimitrios Mougiakakos, Franziska Baenke, Daniel Stange, Roland S. Croner, Michal Linial, Ulf D. Kahlert   +16 more
wiley   +1 more source

In-House RT-PCR Assay for Detection of Human Immunodeficiency Virus Type 1 Infection [PDF]

, 2009
Serologic assays are commonly used for screening (ELISA) and for confirmation (Western blot) of HIV-1 infection; however, both assays have potentially yielded the false-positive or false-negative results.
Bela, B. (Budiman), Fatmawati, N. N. (Ni), Ibrahim, F. (Fera), Parwati, K. T. (Ketut), Yasmon, A. (Andi)   +4 more
core  

Predictors of response and rational combinations for the novel MCL‐1 inhibitor MIK665 in acute myeloid leukemia

Molecular Oncology, EarlyView.
This study characterizes the responses of primary acute myeloid leukemia (AML) patient samples to the MCL‐1 inhibitor MIK665. The results revealed that monocytic differentiation is associated with MIK665 sensitivity. Conversely, elevated ABCB1 expression is a potential biomarker of resistance to the treatment, which can be overcome by the combination ...
Joseph Saad, Rhiannon Newman, Elmira Khabusheva, Sofia Aakko, Eric Durand, Mahesh Tambe, Heikki Kuusanmäki, Alun Parsons, Juho J. Miettinen, Komal Kumar Javarappa, Ezgi June Olgac, Nemo Ikonen, Mika Kontro, Kimmo Porkka, Heiko Maacke, Janghee Woo, Ensar Halilovic, Caroline A. Heckman   +17 more
wiley   +1 more source

Molecular profiling and antimicrobial susceptibility of Escherichia coli O157:H7 isolated in Bulgaria

Bulgarian Journal of Veterinary Medicine, 2020
The purpose of this study was to detect the presence of shiga-toxin producing Escherichia coli (STEC) in faeces of healthy dairy cattle and to determine the sensitivity of isolates to several antimicrobial drugs.
K. Koev, T. Stoyanchev, G. Zhelev, P. Marutsov, K. Gospodinova, V. Urumova   +5 more
doaj   +1 more source

PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products [PDF]

, 2011
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying ...
Abidin, M. Z. (M), Erwanto, Y. (Y), Rohman, A. (A), Sismindari, S. (Sismindari)   +3 more
core  

High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay [PDF]

, 2017
Robert W. Yucha, Kristen S. Hobbs, Emily Hanhauser, Louise E. Hogan, Wildaliz Nieves, Mehmet Ozgün Ozen, Fatih İnci, Vanessa A. York, Erica A. Gibson, Cassandra Thanh, Hadi Shafiee, Rami El Assal, Maja Kiselinova, Yvonne Robles, Helen Bae, Kaitlyn S. Leadabrand, Shuqi Wang, Steven G. Deeks, Daniel R. Kuritzkes, Utkan Demirci, Timothy J. Henrich   +20 more
openalex   +1 more source

Feasibility of a ctDNA multigenic panel for non‐small‐cell lung cancer early detection and disease surveillance

Molecular Oncology, EarlyView.
Plasma‐based detection of actionable mutations is a promising approach in lung cancer management. Analysis of ctDNA with a multigene NGS panel identified TP53, KRAS, and EGFR as the most frequently altered, with TP53 and KRAS in treatment‐naïve patients and TP53 and EGFR in previously treated patients.
Giovanna Maria Stanfoca Casagrande, Marcela de Oliveira Silva, Mariana Bisarro dos Reis, Rodrigo de Oliveira Cavagna, Luciane Sussuchi, Icaro Alves Pinto, Natalia Zampieri Pontes, Rodrigo Sampaio Chiarantano, Flavio Augusto Ferreira da Silva, Pedro de Marchi, Letícia Ferro Leal, Rui M. Reis   +11 more
wiley   +1 more source

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data [PDF]

, 2010
Manuela Marullo, Chiara Zuccato, Caterina Mariotti, Nayana Lahiri, Sarah J. Tabrizi, Stefano Di Donato, Elena Cattaneo   +6 more
openalex   +1 more source

Monitoring COVID-19 Transmission Risks by Quantitative Real-Time PCR Tracing of Droplets in Hospital and Living Environments

, 2021
Andrea Piana, Maria Eugenia Colucci, Federica Valeriani, Adriano Marcolongo, Giovanni Sotgiu, Cesira Pasquarella, Lory Marika Margarucci, Andrea Petrucca, Gianluca Gianfranceschi, Sergio Babudieri, Pietro Vitali, Giuseppe D’Ermo, Assunta Bizzarro, Flavio De Maio, Matteo Vitali, Antonio Azara, Ferdinando Romano, Maurizio Simmaco, Vincenzo Romano Spica   +18 more
openalex   +1 more source