Results 161 to 170 of about 3,602,392 (295)

PCR amplification from paraffin-embedded tissues: recommendations on fixatives for long-term storage and prospective studies. [PDF]

, 1991
Catherine E. Greer, Jennifer K. Lund, M. Michele Manos   +2 more
openalex   +1 more source

PoDCall: a robust tool for automated droplet classification in DNA‐methylation droplet digital PCR

Molecular Oncology, EarlyView.
Hans Petter Brodal, Heidi Pharo, Hege Marie Vedeld, Guro E. Lind   +3 more
wiley   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam. [PDF]

J Vet Diagn Invest
Bui APN, Nguyen NM, Le Q.
europepmc   +1 more source

Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase [PDF]

, 1991
Yuhong Zhou, X P Zhang, Richard H. Ebright   +2 more
openalex   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Molecular diagnostic technology: beyond analytical accuracy. [PDF]

Crit Care
Li X, Liu J.
europepmc   +1 more source

Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting. [PDF]

, 1992
Paul Garrity, B Wold
openalex   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

Preoperative assessment using a scoring system after neoadjuvant chemotherapy with docetaxel, cisplatin, and 5-fluorouracil for locally advanced esophageal cancer: Who can avoid surgery? [PDF]

PLoS One
Kurahashi Y, Tomita T, Okugawa T, Kitajima K, Murakami M, Kohno S, Hojo Y, Nakao E, Nakamura T, Ishida Y, Shinzaki S, Shinohara H.   +11 more
europepmc   +1 more source