Results 51 to 60 of about 4,873,808 (393)
Telomere measurement by quantitative PCR.
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present
R. Cawthon
semanticscholar +1 more source
Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi [PDF]
Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T.
Leal, Danielle C.+4 more
openaire +5 more sources
Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based
Imran Amin+3 more
doaj +1 more source
Applying multi-criteria optimisation to develop cognitive models [PDF]
A scientific theory is developed by modelling empirical data in a range of domains. The goal of developing a theory is to optimise the fit of the theory to as many experimental settings as possible, whilst retaining some qualitative properties such as ...
Gobet, F, Lane, PCR
core
Westward propagating twin gyres in the equatorial Indian Ocean [PDF]
A reduced-gravity (11/2-layer) model forced by daily climatological winds simulates twin, anticyclonic gyres, which propagate westward on either side of the equator.
Deo, AA+3 more
core +1 more source
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth+6 more
semanticscholar +1 more source
Social context prevents heat hormetic effects against mutagens during fish development
This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere+5 more
wiley +1 more source
Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]
Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani+1 more
doaj +1 more source
Towards a model of expectation-driven perception [PDF]
Human perception is an active process by which meaningful information is gathered from the external environment. Application areas such as human-computer interaction (HCI), or the role of human experts in image analysis, highlight the need to ...
Gobet, F, Lane, PCR
core
Rapid Optical Cavity PCR. [PDF]
Recent outbreaks of deadly infectious diseases, such as Ebola and Middle East respiratory syndrome coronavirus, have motivated the research for accurate, rapid diagnostics that can be administered at the point of care.
Gustafson, Lars+6 more
core +2 more sources