Results 51 to 60 of about 3,342,563 (345)
FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
Identification of Listeria Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis
Microbiology ResearchSpecies identification of Listeria isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus Listeria.
Antonio Martínez-Murcia +2 more
doaj +1 more source
Journal of Medical Virology, 2020 In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing ...
Yafang Li +6 more
semanticscholar +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
BioMedica, 2020 Coronavirus disease-2019 (COVID-19) outbreak was first declared as emergency on January 30th2020 by World Health Organization (WHO). The Government of Pakistan presented a National action plan, which aimed to inhibit the spread of this Corona virus and ...
Ghazala Rubi, Irfan Malik, Zainab Javaid
doaj +1 more source
Research in Agricultural Sciences, 2023 During survey, 12 Jackal jujube plant samples showing the symptoms of witches’ broom disease were collected from the Shivamogga district of Karnataka, India, between 2017 and 2018. The causal agent associated with Jackal jujube witches’ broom disease was
C. N. Lakshmınarayana Reddy +5 more
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Genome Research, 1996 We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
Christian A. Heid +3 more
semanticscholar +1 more source
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Nature Communications, 2020 Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki +12 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley +1 more source
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source