Results 51 to 60 of about 4,873,808 (393)

Telomere measurement by quantitative PCR.

open access: yesNucleic Acids Research, 2002
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present
R. Cawthon
semanticscholar   +1 more source

Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi [PDF]

open access: yesPesquisa Veterinária Brasileira, 2011
Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T.
Leal, Danielle C.   +4 more
openaire   +5 more sources

Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

open access: yesInternational Journal of Analytical Chemistry, 2015
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based
Imran Amin   +3 more
doaj   +1 more source

Applying multi-criteria optimisation to develop cognitive models [PDF]

open access: yes, 2005
A scientific theory is developed by modelling empirical data in a range of domains. The goal of developing a theory is to optimise the fit of the theory to as many experimental settings as possible, whilst retaining some qualitative properties such as ...
Gobet, F, Lane, PCR
core  

Westward propagating twin gyres in the equatorial Indian Ocean [PDF]

open access: yes, 2004
A reduced-gravity (11/2-layer) model forced by daily climatological winds simulates twin, anticyclonic gyres, which propagate westward on either side of the equator.
Deo, AA   +3 more
core   +1 more source

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

open access: yesNucleic Acids Research, 2012
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth   +6 more
semanticscholar   +1 more source

Social context prevents heat hormetic effects against mutagens during fish development

open access: yesFEBS Letters, EarlyView.
This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere   +5 more
wiley   +1 more source

Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2020
Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani   +1 more
doaj   +1 more source

Towards a model of expectation-driven perception [PDF]

open access: yes, 2003
Human perception is an active process by which meaningful information is gathered from the external environment. Application areas such as human-computer interaction (HCI), or the role of human experts in image analysis, highlight the need to ...
Gobet, F, Lane, PCR
core  

Rapid Optical Cavity PCR. [PDF]

open access: yes, 2015
Recent outbreaks of deadly infectious diseases, such as Ebola and Middle East respiratory syndrome coronavirus, have motivated the research for accurate, rapid diagnostics that can be administered at the point of care.
Gustafson, Lars   +6 more
core   +2 more sources

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