Results 51 to 60 of about 3,284,156 (346)

False negative of RT‐PCR and prolonged nucleic acid conversion in COVID‐19: Rather than recurrence

open access: yesJournal of Medical Virology, 2020
A novel coronavirus (COVID-19) pandemic cause by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) threatens the world. We read with interest the recent report by Li et al.
Ai Tang Xiao, Y. Tong, S. Zhang
semanticscholar   +1 more source

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

open access: yesFEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

open access: yesNature Communications, 2020
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki   +12 more
semanticscholar   +1 more source

Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy

open access: yesFEBS Letters, EarlyView.
Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile   +8 more
wiley   +1 more source

Identification of Listeria Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis

open access: yesMicrobiology Research
Species identification of Listeria isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus Listeria.
Antonio Martínez-Murcia   +2 more
doaj   +1 more source

Penerapan Metode UCD (User Centered Design) Pada E-Commerce Putri Intan Shop Berbasis Web

open access: yesJurnal Teknologi dan Sistem Informasi, 2017
Pertumbuhan pesat pangsa pasar e-commerce di Indonesia memang sudah tidak bisa diragukan lagi. Dengan jumlah pengguna internet yang mencapai angka 82 juta orang atau sekitar 30% dari total penduduk di Indonesia, pasar e-commerce menjadi tambang emas yang
Intan Sandra Yatana Saputri   +2 more
doaj   +1 more source

COVID-19 in Pakistan

open access: yesBioMedica, 2020
Coronavirus disease-2019 (COVID-19) outbreak was first declared as emergency on January 30th2020 by World Health Organization (WHO). The Government of Pakistan presented a National action plan, which aimed to inhibit the spread of this Corona virus and ...
Ghazala Rubi, Irfan Malik, Zainab Javaid
doaj   +1 more source

Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi [PDF]

open access: yesPesquisa Veterinária Brasileira, 2011
Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T.
Leal, Danielle C.   +4 more
openaire   +5 more sources

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

open access: yesNucleic Acids Research, 2009
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence
J. Ruijter   +6 more
semanticscholar   +1 more source

Detection of Novel Coronavirus by RT-PCR in Stool Specimen from Asymptomatic Child, China

open access: yesEmerging Infectious Diseases, 2020
We report an asymptomatic child who was positive for a coronavirus by reverse transcription PCR in a stool specimen 17 days after the last virus exposure. The child was virus positive in stool specimens for at least an additional 9 days.
A. Tang   +10 more
semanticscholar   +1 more source

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