Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]
, 2011 Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els +3 more
core +3 more sources
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Nature Communications, 2020 Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki +12 more
semanticscholar +1 more source
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
FEBS Letters, EarlyView.The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
Penerapan Metode UCD (User Centered Design) Pada E-Commerce Putri Intan Shop Berbasis Web
Jurnal Teknologi dan Sistem Informasi, 2017 Pertumbuhan pesat pangsa pasar e-commerce di Indonesia memang sudah tidak bisa diragukan lagi. Dengan jumlah pengguna internet yang mencapai angka 82 juta orang atau sekitar 30% dari total penduduk di Indonesia, pasar e-commerce menjadi tambang emas yang
Intan Sandra Yatana Saputri +2 more
doaj +1 more source
Characterisation of South African field Ehrlichia ruminantium using multilocus sequence typing
Onderstepoort Journal of Veterinary Research, 2023 Heartwater, one of the major tick-borne diseases of some domestic and wild ruminants in Africa, is caused by Ehrlichia ruminantium. The genetic diversity of E.
Zinathi Dlamkile +6 more
doaj +1 more source
Computational models of the development of perceptual expertise [PDF]
, 2007 In a recent article, Palmeri, Wong and Gauthier have argued that computational models may help direct hypotheses about the development of perceptual expertise.
Campitelli, G, Gobet, F, Lane, PCR
core
Detection of Novel Coronavirus by RT-PCR in Stool Specimen from Asymptomatic Child, China
Emerging Infectious Diseases, 2020 We report an asymptomatic child who was positive for a coronavirus by reverse transcription PCR in a stool specimen 17 days after the last virus exposure. The child was virus positive in stool specimens for at least an additional 9 days.
A. Tang +10 more
semanticscholar +1 more source
Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1
FEBS Letters, EarlyView.Tgl1, one of yeast sterol ester hydrolases, had been found on the lipid droplets where sterol esters are mainly stored. This study revealed that Tgl1 is transported into the vacuole depending on the ESCRT‐I–III complex, and that it exhibits intra‐vacuolar sterol ester hydrolase activity.
Takumi Nakatsuji +5 more
wiley +1 more source
Identification of Listeria Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis
Microbiology ResearchSpecies identification of Listeria isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus Listeria.
Antonio Martínez-Murcia +2 more
doaj +1 more source
Parasite, 2021 Taeniasis remains a prevalent public health problem in Thailand. National helminthiasis surveys report only the incidence of Taenia spp. eggs. The ability to differentiate Taenia species using morphological and molecular techniques is vital for ...
Kusolsuk Teera +11 more
doaj +1 more source