Results 61 to 70 of about 3,745,037 (345)

Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]

, 2011
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els, Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc   +3 more
core   +3 more sources

Subpar reporting of pre‐analytical variables in RNA‐focused blood plasma studies

Molecular Oncology, EarlyView.
Pre‐analytical variables strongly influence the analysis of extracellular RNA (cell‐free RNA; exRNA) derived from blood plasma. Their reporting is essential to allow interpretation and replication of results. By evaluating 200 exRNA studies, we pinpoint a lack of reporting pre‐analytical variables associated with blood collection, plasma preparation ...
Céleste Van Der Schueren, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Laudonia Lidia Dipalo, Hetty Hilde Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, Anneleen Decock   +16 more
wiley   +1 more source

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source

Molecular Characterization of Some Virulence Genes and Antibiotic Susceptibility Pattern among Uropathogenic Escherichia coli Isolated from Patient in Zakho City/Iraq

Zanco Journal of Pure and Applied Sciences, 2020
Uropathogenic Escherichia coli (UPEC) is one of the most causative agents which causing urinary tract infections (UTIs) in humans. This study involved the prevalence of the virulence genes among UPEC isolated from patients in various hospitals in Zakho ...
Marwa Subhi Ibrahim, Haval M. Khalid, Wijdan M. S. Mero   +2 more
doaj   +1 more source

Microscopic identification, molecular and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq [PDF]

Iraqi Journal of Veterinary Sciences, 2019
Babesia is one of hemoprotozoan parasite transmitted by arthropod vectors which responsible for causing of Babesiosis disease in bovine worldwide. The present study was designed for microscopic identification, molecular, and phylogenetic analysis of ...
Rashaa Ateaa, Mansour Alkhaled
doaj   +1 more source

Combining low-level perception with expectations in CHREST [PDF]

, 2003
The ability of humans to reliably perceive and recognise objects relies on an interaction between information seen in the visual image and prior expectations.
Gobet, F, Lane, PCR, Sykes, A
core   +1 more source

Clinical applications of next‐generation sequencing‐based ctDNA analyses in breast cancer: defining treatment targets and dynamic changes during disease progression

Molecular Oncology, EarlyView.
Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker, Samantha Hasenleithner, Rupert Bartsch, Simon P. Gampenrieder, Daniel Egle, Christian F. Singer, Gabriel Rinnerthaler, Michael Hubalek, Katja Schmitz, Zsuzsanna Bago‐Horvath, Andreas Petzer, Sonja Heibl, Ellen Heitzer, Marija Balic, Michael Gnant   +14 more
wiley   +1 more source

Genetic epidemiology using whole genome sequencing and haplotype networks revealed the linkage of SARS-CoV-2 infection in nosocomial outbreak

Infection Prevention in Practice, 2021
Summary: Background: A characteristic feature of SARS-CoV-2 is its ability to transmit from pre- or asymptomatic patients, complicating the tracing of infection pathways and causing outbreaks.
Fumihiro Ishikawa, Yuko Udaka, Hideto Oyamada, Keiko Ishino, Issei Tokimatsu, Hironori Sagara, Yuji Kiuchi   +6 more
doaj  

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]

Journal of Clinical and Diagnostic Research, 2014
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj   +1 more source

An investigation into the effect of ageing on expert memory with CHREST [PDF]

, 2007
CHREST is a cognitive architecture that models human perception, learning, memory, and problem solving, and which has successfully simulated numerous human experimental data on chess. In this paper, we describe an investigation into the effects of ageing
Gobet, F, Lane, PCR, Smith, RL
core