Results 71 to 80 of about 200,892 (306)

Preparation of Cyclic Lunasin by Heterologous Recombination Combined with Enzymatic Cyclization and Its Properties

Shipin gongye ke-ji
In order to prepare a cyclic polypeptide cLunasin by the method of total biosynthesis, the linear precursor aLunasin was designed for cLunasin synthesis, and a fusion expression vector with the double tags of MBP and SUMO was constructed.
Fan SHI, Yi HOU, Jinsong ZHAO, Songqing HU   +3 more
doaj   +1 more source

De Novo Autogenic Engineered Living Functional Materials

Advanced Materials, EarlyView.
A versatile platform for de novo autogenic engineered living materials is presented, leveraging protein mining, computational modeling, and synthetic biology. By reprogramming the Escherichia coli curli machinery with β‐solenoid proteins from non‐model bacteria, macroscopic biomaterials with enhanced mechanical properties, UV‐C irradiation protection ...
Hoda M. Hammad, Seth Swarnadeep, Erin C. Jackson, Nicolas Burns, Hongyu Wang, Harrison Priode, Robert B. Moore, Sanket Deshmukh, Avinash Manjula‐Basavanna, Anna M. Duraj‐Thatte   +9 more
wiley   +1 more source

Naphthyridinomycin Biosynthesis Revealing the Use of Leader Peptide to Guide Nonribosomal Peptide Assembly [PDF]

, 2016
Analysis of naphthyridinomycin gene cluster revealed that this antibiotic is generated by nonribosomal peptide synthetase (NRPS) machinery. However, four modules encoded by two genes do not correspond with the structural units in the final product ...
Yue Zhang (30585), Jin-Yue Pu (1934254), Man-Cheng Tang (1493491), Li-Qiang Song (1934251), Jian-Ping Guo (412563), Qiang Hua (114970), Gong-Li Tang (1829416), Chao Peng (686643)   +7 more
core   +1 more source

Time-Resolved Visualization of Cyanotoxin Synthesis via Labeling by the Click Reaction in the Bloom-Forming Cyanobacteria Microcystis aeruginosa and Planktothrix agardhii

Toxins
In non-ribosomal peptide synthesis of cyanobacteria, promiscuous adenylation domains allow the incorporation of clickable non-natural amino acids into peptide products—namely into microcystins (MCs) or into anabaenopeptins (APs): 4-azidophenylalanine ...
Rainer Kurmayer, Rubén Morón Asensio
doaj   +1 more source

Targeting UXS1‐Dependent Glucuronate Detoxification Potentiates Metformin's Anti‐Tumor Efficacy in Lung Adenocarcinoma

Advanced Science, EarlyView.
This study reveals that metformin promotes glucuronic acid metabolism in lung adenocarcinoma by activating UGDH S476 phosphorylation and enhancing the conversion of UDPG to UDPGA based on metabolomics analysis. Through compound virtual screening, it is found that plantainoside targeting UGDH downstream UXS1 leads to UDPGA toxicity accumulation ...
Qihai Sui, Zhencong Chen, Guangyao Shan, Zhengyang Hu, Xing Jin, Jiaqi Liang, Yanjun Yi, Jiacheng Yin, Haochun Shi, Xifei Jiang, Junjie Xi, Zongwu Lin, Cheng Zhan, Fenghao Sun, Wei Jiang   +14 more
wiley   +1 more source

Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis [PDF]

, 2016
In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%.
Guglielmi, Danila A. S. [UNESP], Rissi, Nathalia C. [UNESP], Chiavacci, Leila A. [UNESP], Cilli, Eduardo M. [UNESP], Soares, Christiane P. [UNESP], Martinelli, Ariane M. [UNESP]   +5 more
core   +3 more sources

Reiterative Synthesis by the Ribosome and Recognition of the N-Terminal Formyl Group by Biosynthetic Machinery Contribute to Evolutionary Conservation of the Length of Antibiotic Microcin C Peptide Precursor

mBio, 2019
Microcin C (McC) is a peptide adenylate antibiotic produced by Escherichiacoli cells bearing a plasmid-borne mcc gene cluster. Most MccA precursors, encoded by validated mcc operons from diverse bacteria, are 7 amino acids long, but the significance of ...
Inna Zukher, Michael Pavlov, Darya Tsibulskaya, Alexey Kulikovsky, Tatyana Zyubko, Dmitry Bikmetov, Marina Serebryakova, Satish K. Nair, Måns Ehrenberg, Svetlana Dubiley, Konstantin Severinov   +10 more
doaj   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

ANTIBACTERIAL ACTIVITY OF A SECONDARY METABOLITE-PRODUCING CORAL BACTERIUM Pseudoalteromonas SPECIES [PDF]

, 2004
A bacterium, collected at the surface of coral Acropora sp., TAB4.2 was successfully screened for secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase gene.
Karna Radjasa, Okky, Grossart, Hans-Peter, Sabdono, Agus, Marten, Torben, Brinkoff, Thorsten, Simon, Meinhard   +5 more
core  

Mining microbial genomes for new natural products and biosynthetic pathways [PDF]

, 2008
Analyses of microbial genome sequences have revealed numerous examples of ‘cryptic’ or ‘orphan’ biosynthetic gene clusters, with the potential to direct the production of novel, structurally complex natural products.
G. L. Challis, Challis, Gregory L.
core   +1 more source