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Digestion of Peptidoglycan and Analysis of Soluble Fragments
Peptidoglycan (murein) is a vital component of the cell wall of nearly all bacteria, composed of sugars linked by short peptides. This protocol describes the purification of macromolecular peptidoglycan from cultured bacteria and the analysis of enzyme ...
Ryan Schaub, Joseph Dillard
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IntroductionMycobacteria assemble a complex cell wall with cross-linked peptidoglycan (PG) which plays an essential role in maintenance of cell wall integrity and tolerance to osmotic pressure.
Moagi T. Shaku+6 more
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Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus) [PDF]
This work was supported by the National Natural Science Foundation of China (Grant no. 31302221, 31172408 and 31272666) and Jiangsu Province (Grant no.
Chang, Mingxian+8 more
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Staphylococcus aureus DivIB is a peptidoglycan-binding protein that is required for a morphological checkpoint in cell division [PDF]
Bacterial cell division is a fundamental process that requires the coordinated actions of a number of proteins which form a complex macromolecular machine known as the divisome.
Bottomley, Amy L.+5 more
core +1 more source
ABSTRACT Bacterial endospores possess multiple integument layers, one of which is the cortex peptidoglycan wall. The cortex is essential for the maintenance of spore core dehydration and dormancy and contains structural modifications that differentiate it from vegetative cell peptidoglycan and determine its fate during spore ...
David L, Popham, Casey B, Bernhards
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Peptidoglycan: a post-genomic analysis [PDF]
Abstract Background To derive post-genomic, neutral insight into the peptidoglycan (PG) distribution among organisms, we mined 1,644 genomes listed in the Carbohydrate-Active Enzymes database for the presence of a minimal 3-gene set that is necessary for PG metabolism.
Cayrou, Caroline+4 more
openaire +5 more sources
Resistance to mucosal lysozyme compensates for the fitness deficit of peptidoglycan modifications by Streptococcus pneumoniae. [PDF]
The abundance of lysozyme on mucosal surfaces suggests that successful colonizers must be able to evade its antimicrobial effects. Lysozyme has a muramidase activity that hydrolyzes bacterial peptidoglycan and a non-muramidase activity attributable to ...
Kimberly M Davis+3 more
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Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing [PDF]
Airway infection by the Gram-positive pathogen Streptococcus pneumoniae (Sp) leads to recruitment of neutrophils but limited bacterial killing by these cells.
Clarke, Thomas B.+6 more
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DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
Gram-negative bacteria have a complex cell envelope with two membranes and a periplasm containing the peptidoglycan layer. The outer membrane is firmly connected to the peptidoglycan by highly abundant proteins.
Matthias Winkle+8 more
semanticscholar +1 more source
The shape, elongation, division and sporulation (SEDS) proteins are a highly conserved family of transmembrane glycosyltransferases that work in concert with class B penicillin-binding proteins (bPBPs) to build the bacterial peptidoglycan cell wall 1 – 6
M. Sjodt+13 more
semanticscholar +1 more source