Phenotypic and genotypic characterization of peptidoglycan hydrolases of Lactobacillus sakei [PDF]
Journal of Advanced Research, 2016 Lactobacillus sakei, a lactic acid bacterium naturally found in fresh meat and sea products, is considered to be one of the most important bacterial species involved in meat fermentation and bio-preservation. Several enzymes of Lb.
Afef Najjari +6 more
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Identification of key peptidoglycan hydrolases for morphogenesis, autolysis, and peptidoglycan composition of
Microbial Cell Factories, 2012 Background Lactobacillus plantarum is commonly used in industrial fermentation processes. Selected strains are also marketed as probiotics for their health beneficial effects.
Rolain Thomas +6 more
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False positives in using the zymogram assay for identification of peptidoglycan hydrolases. [PDF]
Anal Biochem, 2018 Zymogram assays have been used extensively to identify novel peptidoglycan hydrolases. In this study it is reported that the zymogram is susceptible to false positive results when highly positively charged proteins are assayed. As an example, we report on the case of the ChiZ membrane protein from the Mycobacterium tuberculosis divisome, which ...
Escobar CA, Cross TA.
europepmc +6 more sources
Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases [PDF]
Microbiology Spectrum, 2022 Peptidoglycan (PG) hydrolases, due to their crucial role in the metabolism of the bacterial cell wall (CW), are increasingly being considered suitable targets for therapies, and a potent alternative to conventional antibiotics.
Alicja Wysocka +3 more
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Peptidoglycan Hydrolases of Local Lactic Acid Bacteria from Kazakh Traditional Food [PDF]
Central Asian Journal of Global Health, 2014 Introduction: Peptidoglycan (PG) is a major component of the cell wall of Gram-positive bacteria and is essential for maintaining the integrity of the bacterial cell and its shape.
Serik Shaikhin +6 more
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The Protozoan
mBio, 2018 The human eukaryotic pathogen Trichomonas vaginalis causes trichomoniasis, a prevalent sexually transmitted infection. This extracellular protozoan is intimately associated with the human vaginal mucosa and microbiota, but key aspects of the complex ...
Jully Pinheiro +8 more
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Zymogram Assay for the Detection of Peptidoglycan Hydrolases in Streptococcus mutans
Bio-Protocol, 2013 Peptidoglycan hydrolases or autolysins are enzymes capable of cleaving covalent bonds in bacterial peptidoglycan cell wall layer. They can participate in the cell division process, in the release of turnover products from peptidoglycan during cell growth,
Delphine Dufour, Céline Lévesque
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Author Correction: Coordinated peptidoglycan synthases and hydrolases stabilize the bacterial cell wall [PDF]
Nature CommunicationsHuan Zhang +3 more
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Coordinated peptidoglycan synthases and hydrolases stabilize the bacterial cell wall
Nature Communications, 2023 Peptidoglycan (PG) defines cell shape and protects bacteria against osmotic stress. The growth and integrity of PG require coordinated actions between synthases that insert new PG strands and hydrolases that generate openings to allow the insertion ...
Huan Zhang +3 more
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Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease [PDF]
The Cell SurfaceA defining feature of bacteria is the peptidoglycan cell wall which provides structural integrity and prevents osmotic lysis. While peptidoglycan hydrolases are required for daughter cell separation, dysregulated cell wall degradation may result in cell ...
Viktor H. Mebus +14 more
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