Peptide Bond Formation between Aminoacyl-Minihelices by a Scaffold Derived from the Peptidyl Transferase Center [PDF]
Life, 2022 The peptidyl transferase center (PTC) in the ribosome is composed of two symmetrically arranged tRNA-like units that contribute to peptide bond formation.
Mai Kawabata +5 more
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The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis [PDF]
Nature CommunicationsDEAD-box ATPases play crucial roles in guiding rRNA restructuring events during the biogenesis of large (60S) ribosomal subunits, but their precise molecular functions are currently unknown.
Victor E. Cruz +3 more
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Translational impacts of enzymes that modify ribosomal RNA around the peptidyl transferase centre [PDF]
RNA BiologyLarge ribosomal RNAs (rRNAs) are modified heavily post-transcriptionally in functionally important regions but, paradoxically, individual knockouts (KOs) of the modification enzymes have minimal impact on Escherichia coli growth. Furthermore, we recently
Letian Bao +5 more
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SecM-stalled ribosomes adopt an altered geometry at the peptidyl transferase center. [PDF]
PLoS Biology, 2011 As nascent polypeptide chains are synthesized, they pass through a tunnel in the large ribosomal subunit. Interaction between specific nascent chains and the ribosomal tunnel is used to induce translational stalling for the regulation of gene expression.
Shashi Bhushan +7 more
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Stepwise maturation of the peptidyl transferase region of human mitoribosomes [PDF]
Nature Communications, 2021 Mammalian mitoribosomes feature dramatically reduced ribosomal RNAs and follow mitochondria specific assembly pathways. Here the authors describe the process of human mitochondrial ribosome maturation that results in the formation of the ribosomal active
Tea Lenarčič +9 more
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Peptidyl transferase center decompaction and structural constraints during early protein elongation on the ribosome [PDF]
Scientific Reports, 2021 Peptide bond formation on the ribosome requires that aminoacyl-tRNAs and peptidyl-tRNAs are properly positioned on the A site and the P site of the peptidyl transferase center (PTC) so that nucleophilic attack can occur.
Bin Jia, Tianlong Wang, Jean Lehmann
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Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination [PDF]
Scientific Reports, 2018 The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome.
Fuxing Zeng, Hong Jin
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Origin and evolution of the Peptidyl Transferase Center from proto-tRNAs [PDF]
FEBS Open Bio, 2014 We tested the hypothesis of Tamura (2011) [3] that molecules of tRNA gave origin to ribosomes, particularly to the Peptidyl Transferase Center (PTC) of the 23S ribosomal RNA.
Sávio T. Farias +2 more
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The Ancient History of Peptidyl Transferase Center Formation as Told by Conservation and Information Analyses [PDF]
Life, 2020 The peptidyl transferase center (PTC) is the catalytic center of the ribosome and forms part of the 23S ribosomal RNA. The PTC has been recognized as the earliest ribosomal part and its origins embodied the First Universal Common Ancestor (FUCA). The PTC
Francisco Prosdocimi +4 more
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The Photochemical Inactivation of Peptidyl Transferase Activity [PDF]
European Journal of Biochemistry, 1975 The photochemical oxidation of the 50‐S ribosomal subunit results in a rapid irreversible loss of peptidyl transferase activity. The first‐order rate of inactivation occurring during the first forty minutes suggests that a single reactive group is being inactivated.
Kwong K. WAN, Nasir Zahid, R.M. Baxter
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