Results 41 to 50 of about 1,388 (159)

Repurposing rabeprazole sodium as an anti-Clostridium perfringensdrug by inhibiting perfringolysin O

Journal of Applied Microbiology, 2023
AbstractAimsClostridium perfringens infections affect food safety, human health, and the development of the poultry feed industry. Anti-virulence is an alternative strategy to develop new drug. Perfringolysin O (PFO) is an exotoxin of C. perfringens that has been demonstrated to play critical roles in the pathogenesis of this organism, promising it an ...
Guizhen, Wang, Yan, Liu, Le, Deng, Hongtao, Liu, Xuming, Deng, Quanshun, Li, Haihua, Feng, Zhimin, Guo, Jiazhang, Qiu   +8 more
openaire   +2 more sources

Immunoinformatic analysis of the whole proteome for vaccine design: An application to Clostridium perfringens

Frontiers in Immunology, 2022
Clostridium perfringens is a dangerous bacterium and known biological warfare weapon associated with several diseases, whose lethal toxins can produce necrosis in humans. However, there is no safe and fully effective vaccine against C.
Luis F. Soto, Ana C. Romaní, Gabriel Jiménez-Avalos, Yshoner Silva, Carla M. Ordinola-Ramirez, Rainer M. Lopez Lapa, Rainer M. Lopez Lapa, David Requena   +7 more
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Capacity of listeriolysin O, streptolysin O, and perfringolysin O to mediate growth of Bacillus subtilis within mammalian cells [PDF]

Infection and Immunity, 1992
The Listeria monocytogenes hemolysin listeriolysin O (LLO) plays a major role in mediating the escape of L. monocytogenes from a vacuolar compartment. In a previous report, it was shown that Bacillus subtilis expressing LLO could escape from a host vacuolar compartment and grow in the cytoplasm (J. Bielecki, P. Youngman, P. Connelly, and D. A. Portnoy,
D A, Portnoy, R K, Tweten, M, Kehoe, J, Bielecki   +3 more
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Perfringolysin O as a useful tool to study human sperm physiology

Fertility and Sterility, 2013
To evaluate perfringolysin O, a cholesterol-dependent pore-forming cytolysin, as a tool to study several aspects of human sperm physiology.Prospective study.Basic research laboratory.Human semen samples with normal parameters obtained from healthy donors.Interaction of recombinant perfringolysin O with human spermatozoa.Assessment of perfringolysin O ...
Pocognoni, Cristián Adrián, de Blas, Gerardo Andrés, Heuck, Alejandro P., Belmonte, Silvia Alejandra, Mayorga, Luis Segundo   +4 more
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Characterization of Listeria monocytogenes pathogenesis in a strain expressing perfringolysin O in place of listeriolysin O [PDF]

Infection and Immunity, 1994
Listeriolysin O (LLO) is a pore-forming cytolysin that enables Listeria monocytogenes to escape from a host cell vacuole. The structural gene for the related cytolysin perfringolysin O (pfo) was cloned downstream from the promoter for hly, the gene encoding LLO, both on a plasmid and on the L. monocytogenes chromosome. Both strains secreted active PFO,
S, Jones, D A, Portnoy
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Streptococcus pyogenes NAD+-Glycohydrolase Reduces Skeletal Muscle βNAD+ Levels Independently of Streptolysin O

Microorganisms, 2022
Necrotizing soft tissue infections caused by Streptococcus pyogenes (group A streptococcus [GAS]) are characterized by rapid and extensive necrosis of fascia and muscle.
Eric R. McIndoo, Emily Price, Cheri L. Lamb, Christopher S. Dayton, Clifford R. Bayer, Dennis L. Stevens, Amy E. Bryant, Sarah E. Hobdey   +7 more
doaj   +1 more source

R468A mutation in perfringolysin O destabilizes toxin structure and induces membrane fusion

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2017
Perfringolysin O (PFO) belongs to the family of cholesterol-dependent cytolysins. Upon binding to a cholesterol-containing membrane, PFO undergoes a series of structural changes that result in the formation of a β-barrel pore and cell lysis. Recognition and binding to cholesterol are mediated by the D4 domain, one of four domains of PFO.
Magdalena, Kulma, Aleksandra, Kacprzyk-Stokowiec, Katarzyna, Kwiatkowska, Gabriela, Traczyk, Andrzej, Sobota, Michał, Dadlez   +5 more
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Listeriolysin O is necessary and sufficient to induce autophagy during Listeria monocytogenes infection. [PDF]

PLoS ONE, 2010
Recent studies have suggested that autophagy is utilized by cells as a protective mechanism against Listeria monocytogenes infection.However we find autophagy has no measurable role in vacuolar escape and intracellular growth in primary cultured bone ...
Nicole Meyer-Morse, Jennifer R Robbins, Chris S Rae, Sofia N Mochegova, Michele S Swanson, Zijiang Zhao, Herbert W Virgin, Daniel Portnoy   +7 more
doaj   +1 more source

Single-Molecule Imaging of Perfringolysin O Binding and Assembly on Model Membranes [PDF]

Biophysical Journal, 2016
Perfringolysin O (PFO) is the archetypical pore-forming toxin in the cholesterol-dependent cytolysin (CDC) family, which is implicated in major infections ranging from food poisoning to pneumonia and meningitis. Like other CDCs, transmembrane pores are formed when PFO monomers bind to cholesterol-containing membranes and oligomerize into larger (30-50 ...
Senior, Michael J., Monico, Carina, Heuck, Alejandro P., Gilbert, Robert J.C., Wallace, Mark I.   +4 more
openaire   +1 more source

Single molecule compression reveals intra-protein forces drive cytotoxin pore formation

eLife, 2015
Perfringolysin O (PFO) is a prototypical member of a large family of pore-forming proteins that undergo a significant reduction in height during the transition from the membrane-assembled prepore to the membrane-inserted pore. Here, we show that targeted
Daniel M Czajkowsky, Jielin Sun, Yi Shen, Zhifeng Shao   +3 more
doaj   +1 more source