Results 51 to 60 of about 9,422 (217)

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men

Asian Journal of Andrology, 2015
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate ...
Meng Rao, Xiao-Ling Zhao, Jing Yang, Shi-Fu Hu, Hui Lei, Wei Xia, Chang-Hong Zhu   +6 more
doaj   +1 more source

Comprehensive Analysis of the PRDXs Family in Head and Neck Squamous Cell Carcinoma

Frontiers in Oncology, 2022
The peroxidase family of peroxiredoxins (PRDXs) plays a vital role in maintaining the intracellular balance of ROS. However, their function in head and neck squamous cell carcinoma (HNSCC) has not been investigated.
Ruoyan Cao, Ruoyan Cao, Ruoyan Cao, Weilin Zhang, Weilin Zhang, Weilin Zhang, Hongjian Zhang, Hongjian Zhang, Hongjian Zhang, Lixuan Wang, Lixuan Wang, Lixuan Wang, Xijuan Chen, Xijuan Chen, Xijuan Chen, Xianyue Ren, Xianyue Ren, Xianyue Ren, Bin Cheng, Bin Cheng, Bin Cheng, Juan Xia, Juan Xia, Juan Xia   +23 more
doaj   +1 more source

Host Cell Protein Challenges in Antibody Processing: Insights Into Persistence and Mitigation Strategies

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Persistence of HCPs remains a major purification challenge in biopharmaceutical manufacturing with direct consequences for product quality, patient safety, and regulatory compliance. Over the last two decades, deeper insights into the mechanisms driving HCP persistence have clarified why certain proteins evade removal during purification ...
Younghoon Oh, Andrew Cooke, Harshal Soni, Susan Liu, Mamadou Bah, Brianna Bellis, Laura Smith, Michael Capaldi, Nicholas Levy, Erik Wilhelm, Kristopher Barnthouse   +10 more
wiley   +1 more source

Peroxiredoxin Catalysis at Atomic Resolution [PDF]

Structure, 2016
Peroxiredoxins (Prxs) are ubiquitous cysteine-based peroxidases that guard cells against oxidative damage, are virulence factors for pathogens, and are involved in eukaryotic redox regulatory pathways. We have analyzed catalytically active crystals to capture atomic resolution snapshots of a PrxQ subfamily enzyme (from Xanthomonas campestris ...
Perkins, Arden, Parsonage, Derek, Nelson, Kimberly J., Ogba, O. Maduka, Cheong, Paul Ha-Yeon, Poole, Leslie B., Karplus, P. Andrew   +6 more
openaire   +3 more sources

Thiol Peroxidases as Major Regulators of Intracellular Levels of Peroxynitrite in Live Saccharomyces cerevisiae Cells

Antioxidants, 2020
Thiol peroxidases (TP) are ubiquitous and abundant antioxidant proteins of the peroxiredoxin and glutathione peroxidase families that can catalytically and rapidly reduce biologically relevant peroxides, such as hydrogen peroxide and peroxynitrite ...
André Luís Condeles, Fernando Gomes, Marcos Antonio de Oliveira, Luís Eduardo Soares Netto, José Carlos Toledo Junior   +4 more
doaj   +1 more source

Protective Effects of H₂S Donor Treatment in Experimental Colitis: A Focus on Antioxidants

Antioxidants, 2023
Inflammatory bowel diseases (IBD) are chronic, inflammatory disorders of the gastrointestinal (GI) system, which have become a global disease over the past few decades.
Szilvia Török, Nikoletta Almási, Médea Veszelka, Denise Börzsei, Renáta Szabó, Csaba Varga   +5 more
doaj   +1 more source

Heat stress in spermatogenesis: a redox‐dependent decision network linking ROS, autophagy, and regulated cell death

Biological Reviews, EarlyView.
ABSTRACT Heat stress (HS) is an increasing threat to male reproductive health, disrupting spermatogenesis through complex and interdependent cellular responses within the seminiferous epithelium. Although excessive reactive oxygen species (ROS) production is a hallmark of HS, ROS also act as central regulators of cell fate decisions beyond their role ...
Ribrio Ivan Tavares Pereira Batista
wiley   +1 more source

Targeting the intratumoral microbiota to overcome cancer immunotherapy resistance

Interdisciplinary Medicine, EarlyView.
This review delineates the mechanisms of intratumoral microbiota in cancer immunotherapy resistance, and offers strategies for microbiota‐targeted precision immunotherapy. Abstract Cancer remains a significant global health threat. The tumor microenvironment (TME) is a sophisticated ecological niche that exerts a pivotal effect on treatment outcomes ...
Bufu Tang, Tong Jiang, Yaling Lin, Wei Zhang, Hongjie Fan, Minjie Yang, Bingzhe Li, Yaozu Liu, Changyu Li, Xuran Jin, Wen Zhang, Zhiping Yan, Qianqian Zhao, Xuefeng Kan, Xudong Qu   +14 more
wiley   +1 more source

The paternal genome and the health of the assisted reproductive technology child

Asian Journal of Andrology, 2015
As a number of children born by assisted reproductive technology (ART) are increasing each year across the developed world, the health of such offspring is a matter of public concern.
Sheena EM Lewis, Kishlay Kumar
doaj   +1 more source