Functional genomics with a comprehensive library of transposon mutants for the sulfate-reducing bacterium Desulfovibrio alaskensis G20. [PDF]
UnlabelledThe genomes of sulfate-reducing bacteria remain poorly characterized, largely due to a paucity of experimental data and genetic tools. To meet this challenge, we generated an archived library of 15,477 mapped transposon insertion mutants in the
Arkin, Adam P +11 more
core +2 more sources
How super is supersulfide?: Reconsidering persulfide reactivity in cellular biology
In an attempt to understand the cellular mechanisms of H2S signalling, recent research has focused on supersulfide (i.e., alkyl and inorganic hydropersulfide) formation and subsequent reactivity.
Christopher H. Switzer
doaj +1 more source
Haloferax volcanii UbaA, catalytic engine for sampylation and sulfur transfer [PDF]
The Small Archaeal Modifier Proteins (SAMPs) from Haloferax volcanii belong to the group of ubiquitin like proteins (Ubls) that act both as protein modifiers and sulfur carriers.
Bochtler, Matthias, Piasecka, Anna
core +1 more source
A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems [PDF]
Protein Persulfide Detection Protocol reveals vital roles for thioredoxin and glutathione systems in maintaining sulfane sulfur homeostasis in cells and in vivo.
Éva Dóka +11 more
openaire +3 more sources
We demonstrate that Cars2, a cysteine catabolic enzyme in mouse iBAT, is critical for cold tolerance and brown adipocyte differentiation. Through its CPERS activity, Cars2 produces CysSSH/H2S to induce EBF2 persulfidation, promoting its interaction with PPARγ and BRG1 to enhance thermogenic gene expression.
Xin Peng +8 more
wiley +1 more source
A subpopulation of MDCK (NBL‐2) cells, an epithelial cell line derived from the normal kidney, expresses DAO and 3MST. The DAO/3MST‐expressing cells are progressively eliminated during passaging; however, clonal isolation enables the establishment of sublines that maintain stable H2S production. Clonal cells co‐expressing DAO and 3MST therefore provide
Akari Miyamoto +4 more
wiley +1 more source
Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway [PDF]
The phenotypic features of the Azotobacter vinelandii RhdA mutant MV474 (in which the rhdA gene was deleted) indicated that defects in antioxidant systems in this organism were related to the expression of the tandem-domain rhodanese RhdA.
Forlani, Fabio +5 more
core +4 more sources
NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2
The loss of NFS1 leads to its reduced binding with the transcription factor SP2, resulting in decreased expression of the downstream gene Idh2. This causes a shift in energy supply toward glycolysis in VSMCs, ultimately contributing to the occurrence and progression of AAA.
Luzheng Zhang +9 more
wiley +1 more source
We recently identified the novel persulfide sensor SqrR that functions as a master regulator of sulfide-dependent gene expression in the purple photosynthetic bacterium Rhodobacter capsulatus. SqrR binds to the promoter regions of target genes to repress
Takayuki Shimizu, Shinji Masuda
doaj +1 more source
A Proteomic Approach to Study the Effect of Thiotaurine on Human Neutrophil Activation [PDF]
Thiotaurine, a thiosulfonate related to taurine and hypotaurine, is formed by a metabolic process from cystine and generated by a transulfuration reaction between hypotaurine and thiocysteine.
A Ferrante +33 more
core +2 more sources

