Results 21 to 30 of about 194,872 (203)

Plastics and Bioplastics End of Life: A Biocatalytic Perspective. [PDF]

Microb Biotechnol
Biocatalysis links selected conventional plastics and bioplastics to biological routes for polymer transformation, product recovery and end‐of‐life management. Enzymes and microbes enable depolymerisation, upcycling and recovery of monomers, platform chemicals and new materials.
de Eugenio LI, Pardo I, Prieto MA.
europepmc   +2 more sources

Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon Haloferax mediterranei: Key Enzyme of Biodegradable Plastic Synthesis [PDF]

Bioengineering
The biosynthesis of polyhydroxyalkanoate (PHA) biopolymer is highly dependent on the activity of a key enzyme, PHA synthase (PhaC). The halophilic archaeon Haloferax mediterranei can accumulate large amounts of PHAs from different carbon sources under ...
Diya Alsafadi, Yomen Ghalawinji, Fawwaz I. Khalili   +2 more
doaj   +2 more sources

Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase. [PDF]

Appl Environ Microbiol, 2014
ABSTRACT In this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (PHA) synthases (PhaCs) in the presence of phasin proteins (PhaPs), which revealed that PhaPs are activators of PhaC derived from Aeromonas caviae (PhaC
Ushimaru K, Motoda Y, Numata K, Tsuge T.
europepmc   +4 more sources

In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate. [PDF]

Polymers (Basel), 2021
Polyhydroxyalkanoate (PHA) is a biodegradable thermoplastic naturally synthesized by many microorganisms, and the PHA synthase (PhaC) is known to be the key enzyme involved in determining the material properties and monomer composition of the produced ...
Mok PS, Chuah JA, Najimudin N, Liew PW, Jong BC, Sudesh K.   +5 more
europepmc   +2 more sources

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

Applied Sciences (Switzerland), 2017
A recombinant of Pseudomonas putida LS461 (deletion of the phaC1phaZphaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC116 gene from a metagenomic clone. The resulting strain, P.
Warren Blunt, Trevor C Charles, David Levin   +2 more
exaly   +3 more sources

Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria [PDF]

Microbial Cell Factories
Polyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential.
Abdiqani Ibrahim Osman, Brendon Noble, Linda Percy, Pooja Basnett   +3 more
doaj   +2 more sources

Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation.

PLoS ONE, 2022
While plastics have revolutionized our world, plastic waste has serious environmental and economic impacts. Polyhydroxyalkanoic acid (PHA) is a bacterial carbon and energy reserve shown to be both biodegradable and biocompatible and could potentially ...
Dana Kolibachuk, Benjamin J Ryder, Edward R Lyons, Ariel Woolsey, Margaret K Lopes, Keya Thakkar, Mayelin Pacheco Nunez, Michaela Duquenoy, Nevan R Valente, Ashley Nieves   +9 more
doaj   +2 more sources

PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003 [PDF]

Bioengineering, 2020
Among the different tools which can be studied and managed to tailor-make polyhydroxyalkanoates (PHAs) and enhance their production, bacterial strain and carbon substrates are essential. The assimilation of carbon sources is dependent on bacterial strain’s metabolism and consequently cannot be dissociated.
Thomas, Tatiana, Sudesh, Kumar, Bazire, Alexis, Elain, Anne, Tan, Hua Tiang, Lim, Hui, Bruzaud, Stéphane   +6 more
openaire   +3 more sources

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

Frontiers in Bioengineering and Biotechnology, 2022
With the rapid development of synthetic biology, a variety of biopolymers can be obtained by recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one with promising material properties, such as biodegradability and ...
Qiang Gao, Hao Yang, Chi Wang, Xin-Ying Xie, Kai-Xuan Liu, Ying Lin, Shuang-Yan Han, Mingjun Zhu, Markus Neureiter, Yina Lin, Jian-Wen Ye   +10 more
doaj   +1 more source

Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4

Microorganisms, 2022
The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of
Saki Goto, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, Ayaka Hiroe   +4 more
doaj   +1 more source