Results 21 to 30 of about 6,503 (167)

Screening Method for Polyhydroxyalkanoate Synthase Mutants Based on Polyester Degree of Polymerization Using High-Performance Liquid Chromatography

Microorganisms, 2021
A high-throughput screening method based on the degree of polymerization (DP) of polyhydroxyalkanoate (PHA) was developed using high-performance liquid chromatography (HPLC).
Manami Ishii-Hyakutake, Tetsuo Sakurai, Takeharu Tsuge   +2 more
doaj   +1 more source

Polyhydroxyalkanoate (PHA) Production in Pseudomonas sp. phDV1 Strain Grown on Phenol as Carbon Sources

Microorganisms, 2021
Pseudomonas strains have a variety of potential uses in bioremediation and biosynthesis of biodegradable plastics. Pseudomonas sp. strain phDV1, a Gram-negative phenol degrading bacterium, has been found to utilize monocyclic aromatic compounds as sole ...
Iliana Kanavaki, Athina Drakonaki, Ermis Dionisios Geladas, Apostolos Spyros, Hao Xie, Georgios Tsiotis   +5 more
doaj   +1 more source

Polyhydroxyalkanoate Producing Potential of Saccharomyces cerevisiae

Fountain Journal of Natural and Applied Sciences (FUJNAS), 2018
Polyhydroxyalkanoates (PHAs) are inclusion bodies accumulated by some microorganisms as reserve material under unbalanced growth condition such as limited oxygen, nitrogen, phosphorous or sulphur and excess carbon source.
S. O. Jimoh, F. O. Akinwande, O. A. Ayinde, T. Y. Senbadejo   +3 more
doaj   +3 more sources

Characterization of newly isolated thermotolerant bacterium Cupriavidus sp. CB15 from composting and its ability to produce polyhydroxyalkanoate from glycerol

Microbial Cell Factories, 2023
Background This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions.
Anuyut Yootoum, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Churairat Moukamnerd, Thanongsak Chaiyaso, Chayakorn Pumas, Nuttapol Tanadchangsaeng, Masanori Watanabe, Toshiaki Fukui, Chayatip Insomphun   +9 more
doaj   +1 more source

Engineering the Yeast Yarrowia lipolytica for Production of Polylactic Acid Homopolymer

Frontiers in Bioengineering and Biotechnology, 2020
Polylactic acid is a plastic polymer widely used in different applications from printing filaments for 3D printer to mulching films in agriculture, packaging materials, etc.
Sophie Lajus, Simon Dusséaux, Jonathan Verbeke, Coraline Rigouin, Zhongpeng Guo, Maria Fatarova, Floriant Bellvert, Vinciane Borsenberger, Mélusine Bressy, Jean-Marc Nicaud, Alain Marty, Alain Marty, Florence Bordes   +12 more
doaj   +1 more source

RNA-Seq analysis provides insights for understanding photoautotrophic polyhydroxyalkanoate production in recombinant Synechocystis Sp. [PDF]

PLoS ONE, 2014
The photosynthetic cyanobacterium, Synechocystis sp. strain 6803, is a potential platform for the production of various chemicals and biofuels. In this study, direct photosynthetic production of a biopolymer, polyhydroxyalkanoate (PHA), in genetically ...
Nyok-Sean Lau, Choon Pin Foong, Yukio Kurihara, Kumar Sudesh, Minami Matsui   +4 more
doaj   +1 more source

Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

Current Research in Biotechnology, 2022
Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial petrochemical plastics in certain applications like single-use packaging since they are biodegradable, biocompatible and share similar properties with conventional ...
Soon Zher Neoh, Min Fey Chek, Hua Tiang Tan, Javier A. Linares-Pastén, Ardra Nandakumar, Toshio Hakoshima, Kumar Sudesh   +6 more
doaj   +1 more source

Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Microbial Cell Factories, 2017
Background Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins.
Jinping Du, Bernd H. A. Rehm
doaj   +1 more source

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2020
Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaCAR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of
Shuzo Arai, Sayaka Sakakibara, Robin Mareschal, Robin Mareschal, Toshihiko Ooi, Manfred Zinn, Ken’ichiro Matsumoto   +6 more
doaj   +1 more source

Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate

MicrobiologyOpen, 2021
Polyhydroxyalkanoates (PHAs) are biodegradable bioplastics that can be manufactured sustainably and represent a promising green alternative to petrochemical‐based plastics.
Irina Lascu, Ioana Mereuță, Iulia Chiciudean, Hilde Hansen, Sorin Marius Avramescu, Ana‐Maria Tănase, Ileana Stoica   +6 more
doaj   +1 more source