Results 21 to 30 of about 16,047 (206)

Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase. [PDF]

Appl Environ Microbiol, 2014
ABSTRACT In this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (PHA) synthases (PhaCs) in the presence of phasin proteins (PhaPs), which revealed that PhaPs are activators of PhaC derived from Aeromonas caviae (PhaC
Ushimaru K, Motoda Y, Numata K, Tsuge T.
europepmc   +4 more sources

Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers. [PDF]

FEBS Open Bio
Microbial biosynthesis and engineered enzyme platforms are expanding the design space of polyhydroxyalkanoate bioplastics. By combining fermentation, PHA synthase engineering and cell‐free modular systems, tailored biodegradable polymers can be produced with tunable properties, supporting more sustainable materials and future circular bioeconomy ...
Gallo G, Piccoli E, Bombardi L, Aulitto M, Fusco S.   +4 more
europepmc   +2 more sources

Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation.

PLoS ONE, 2022
While plastics have revolutionized our world, plastic waste has serious environmental and economic impacts. Polyhydroxyalkanoic acid (PHA) is a bacterial carbon and energy reserve shown to be both biodegradable and biocompatible and could potentially ...
Dana Kolibachuk, Benjamin J Ryder, Edward R Lyons, Ariel Woolsey, Margaret K Lopes, Keya Thakkar, Mayelin Pacheco Nunez, Michaela Duquenoy, Nevan R Valente, Ashley Nieves   +9 more
doaj   +2 more sources

PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003 [PDF]

Bioengineering, 2020
Among the different tools which can be studied and managed to tailor-make polyhydroxyalkanoates (PHAs) and enhance their production, bacterial strain and carbon substrates are essential. The assimilation of carbon sources is dependent on bacterial strain’s metabolism and consequently cannot be dissociated.
Thomas, Tatiana, Sudesh, Kumar, Bazire, Alexis, Elain, Anne, Tan, Hua Tiang, Lim, Hui, Bruzaud, Stéphane   +6 more
openaire   +3 more sources

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

Frontiers in Bioengineering and Biotechnology, 2022
With the rapid development of synthetic biology, a variety of biopolymers can be obtained by recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one with promising material properties, such as biodegradability and ...
Qiang Gao, Hao Yang, Chi Wang, Xin-Ying Xie, Kai-Xuan Liu, Ying Lin, Shuang-Yan Han, Mingjun Zhu, Markus Neureiter, Yina Lin, Jian-Wen Ye   +10 more
doaj   +1 more source

Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4

Microorganisms, 2022
The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of
Saki Goto, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, Ayaka Hiroe   +4 more
doaj   +1 more source

Two Polyhydroxyalkanoate Synthases from Distinct Classes from the Aromatic Degrader Cupriavidus pinatubonensis JMP134 Exhibit the Same Substrate Preference. [PDF]

PLoS ONE, 2015
Cupriavidus pinatubonensis JMP134 utilizes a variety of aromatic substrates as sole carbon sources, including meta-nitrophenol (MNP). Two polyhydroxyalkanoate (PHA) synthase genes, phaC1 and phaC2, were annotated and categorized as class I and class II ...
Xuan Jiang, Xi Luo, Ning-Yi Zhou
doaj   +1 more source

Heterologous constitutive production of short-chain-length polyhydroxyalkanoates in Pseudomonas putida KT2440: the involvement of IbpA inclusion body protein

Frontiers in Bioengineering and Biotechnology, 2023
Designing cell factories for the production of novel polyhydroxyalkanoates (PHAs) via smart metabolic engineering is key to obtain à la carte materials with tailored physicochemical properties.
Maria-Tsampika Manoli, Maria-Tsampika Manoli, Francisco G. Blanco, Francisco G. Blanco, Virginia Rivero-Buceta, Virginia Rivero-Buceta, Ryan Kniewel, Ryan Kniewel, Sandra Herrera Alarcon, Sandra Herrera Alarcon, Sergio Salgado, Sergio Salgado, M. Auxiliadora Prieto, M. Auxiliadora Prieto   +13 more
doaj   +1 more source

Engineering of Aeromonas caviae Polyhydroxyalkanoate Synthase Through Site-Directed Mutagenesis for Enhanced Polymerization of the 3-Hydroxyhexanoate Unit

Frontiers in Bioengineering and Biotechnology, 2021
Polyhydroxyalkanoate (PHA) synthase is an enzyme that polymerizes the acyl group of hydroxyacyl-coenzyme A (CoA) substrates. Aeromonas caviae PHA synthase (PhaCAc) is an important biocatalyst for the synthesis of a useful PHA copolymer, poly[(R)-3 ...
Ken Harada, Ken Harada, Shingo Kobayashi, Kanji Oshima, Shinichi Yoshida, Takeharu Tsuge, Shunsuke Sato   +6 more
doaj   +1 more source

In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases [PDF]

FEMS Microbiology Letters, 2005
For the first time a functional protein was fused to a PHA synthase resulting in PHA granule formation and display of the respective function at the PHA granule surface. The GFP reporter protein was N-terminally fused to the class I PHA synthase of Cupriavidus necator (PhaC) and the class II PHA synthase of Pseudomonas aeruginosa PAO1 (PhaC1 ...
Peters, V, Rehm, BHA
openaire   +3 more sources