Results 31 to 40 of about 5,996 (169)

RNA-Seq analysis provides insights for understanding photoautotrophic polyhydroxyalkanoate production in recombinant Synechocystis Sp. [PDF]

PLoS ONE, 2014
The photosynthetic cyanobacterium, Synechocystis sp. strain 6803, is a potential platform for the production of various chemicals and biofuels. In this study, direct photosynthetic production of a biopolymer, polyhydroxyalkanoate (PHA), in genetically ...
Nyok-Sean Lau, Choon Pin Foong, Yukio Kurihara, Kumar Sudesh, Minami Matsui   +4 more
doaj   +1 more source

Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

Current Research in Biotechnology, 2022
Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial petrochemical plastics in certain applications like single-use packaging since they are biodegradable, biocompatible and share similar properties with conventional ...
Soon Zher Neoh, Min Fey Chek, Hua Tiang Tan, Javier A. Linares-Pastén, Ardra Nandakumar, Toshio Hakoshima, Kumar Sudesh   +6 more
doaj   +1 more source

Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Microbial Cell Factories, 2017
Background Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins.
Jinping Du, Bernd H. A. Rehm
doaj   +1 more source

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2020
Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaCAR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of
Shuzo Arai, Sayaka Sakakibara, Robin Mareschal, Robin Mareschal, Toshihiko Ooi, Manfred Zinn, Ken’ichiro Matsumoto   +6 more
doaj   +1 more source

Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate

MicrobiologyOpen, 2021
Polyhydroxyalkanoates (PHAs) are biodegradable bioplastics that can be manufactured sustainably and represent a promising green alternative to petrochemical‐based plastics.
Irina Lascu, Ioana Mereuță, Iulia Chiciudean, Hilde Hansen, Sorin Marius Avramescu, Ana‐Maria Tănase, Ileana Stoica   +6 more
doaj   +1 more source

Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

e-Polymers, 2020
Oil reservoirs contain large amounts of hydrocarbon rich produced water, trapped in underground channels. Focus of this study was isolation of PHA producers from produced water concomitant with optimization of production using animal fat and glycerol as ...
Sohail Rafeya, Jamil Nazia, Ali Iftikhar, Munir Sajida   +3 more
doaj   +1 more source

Comparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships

Microbial Cell Factories, 2011
Background Halophilic bacteria have shown their significance in industrial production of polyhydroxyalkanoates (PHA) and are gaining more attention for genetic engineering modification.
Cai Lei, Tan Dan, Aibaidula Gulsimay, Dong Xin-Ran, Chen Jin-Chun, Tian Wei-Dong, Chen Guo-Qiang   +6 more
doaj   +1 more source

Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles

Brazilian Journal of Medical and Biological Research, 2013
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro.
E.V. Cunha, J.J.N. Costa, R.O.D.S. Rossi, A.W.B. Silva, J.R.S. Passos, A.M.L.R. Portela, D.C.S.T. Pereira, M.A.M. Donato, C.C. Campello, M.V.A. Saraiva, C.A. Peixoto, J.R.V. Silva, R.P. Santos   +12 more
doaj   +3 more sources

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

Frontiers in Microbiology, 2021
The accumulation of petroleum-based plastic waste has become a major issue for the environment. A sustainable and biodegradable solution can be found in Polyhydroxyalkanoates (PHAs), a microbially produced biopolymer.
Paton Vuong, Daniel J. Lim, Daniel V. Murphy, Michael J. Wise, Michael J. Wise, Andrew S. Whiteley, Parwinder Kaur   +6 more
doaj   +1 more source

Toward Engineering the Substrate Specificity of a PHA Synthase (PhaC) [PDF]

, 2021
<p>Manufacturing of high-grade plastics from petroleum-based feedstocks is a high-cost, unsustainable process resulting in expensive products. My overall goal was to engineer the pathway of bacterial bio-polyester formation, in order to produce high-grade bioplastics.
openaire   +1 more source