Results 11 to 20 of about 16,047 (206)

Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli [PDF]

Frontiers in Bioengineering and Biotechnology, 2022
Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1Ps6-19) was commonly utilized in the de novo biosynthesis of PLA from biomass.
Mengxun Shi, Mengxun Shi, Mengdi Li, Anran Yang, Xue Miao, Liu Yang, Jagroop Pandhal, Huibin Zou, Huibin Zou   +8 more
doaj   +6 more sources

Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes [PDF]

Microorganisms, 2022
Polyhydroxyalkanoate (PHA), a biodegradable and plastic-like biopolymer, has been receiving research and industrial attention due to severe plastic pollution, resource depletion, and global waste issues.
Athraa Alsaadi, Sree Selva Kumar Ganesen, Tan Suet May Amelia, Radwa Moanis, Eveline Peeters, Sevakumaran Vigneswari, Kesaven Bhubalan   +6 more
doaj   +5 more sources

Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene [PDF]

Microbial Cell Factories, 2012
Background Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate (3HB) monomers and medium-chain-
Gao Xue, Yuan Xiao-Xi, Shi Zhen-Yu, Guo Ying-Ying, Shen Xiao-Wen, Chen Jin-Chun, Wu Qiong, Chen Guo-Qiang   +7 more
doaj   +2 more sources

Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava : PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii [PDF]

Bioresource Technology, 2017
Synthetic wastewater (SW) at various carbon concentrations (5–60 g/l) were evaluated for polyhydroxyalkanoates (PHA) production using the bacteria Pseudomonas pseudoflava.
CHANG Young-Cheol, MAWATARI Yasuteru, NAKAMURA Yuki, ONODERA Rui, REDDY M.Venkateswar, YAJIMA Yuka   +5 more
core   +4 more sources

PHA synthase variant design using a conditional variational autoencoder. [PDF]

PLoS Computational Biology
Polyhydroxyalkanoate (PHA) synthases are a group of complex, dimeric enzymes which catalyze polymerization of R-hydroxyacids into PHAs. PHA properties depend on their monomer composition but enzymes found in nature have limited specificities to certain R-
Tuula Tenkanen, Anna Ylinen, Paula Jouhten, Merja Penttilä, Sandra Castillo   +4 more
doaj   +2 more sources

Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the Extremophile Haloferax mediterranei [PDF]

Marine Drugs
Bioplastics such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are promising alternatives to conventional plastics. However, the high production cost limits their industrial application.
Alexandra Simica, Yolanda Segovia, Alicia Navarro-Sempere, Rosa María Martínez-Espinosa, Carmen Pire   +4 more
doaj   +2 more sources

Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains [PDF]

Microbial Cell Factories, 2010
Background Methylotrophic (methanol-utilizing) bacteria offer great potential as cell factories in the production of numerous products from biomass-derived methanol. Bio-methanol is essentially a non-food substrate, an advantage over sugar-utilizing cell
Miguez Carlos B, Osborne Michael J, Choi Young J, Höfer Philipp, Vermette Patrick, Groleau Denis   +5 more
doaj   +4 more sources

Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition [PDF]

Brazilian Archives of Biology and Technology, 2013
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa.
Abdul Basit Khan, Mehmood Iqbal Khattak, Omer Mukhtar Tarar, Farzana Habib, Khalid Jamil, Arfa Yasmin, Shoukat Parvez   +6 more
doaj   +3 more sources

Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon Haloferax mediterranei: Key Enzyme of Biodegradable Plastic Synthesis [PDF]

Bioengineering
The biosynthesis of polyhydroxyalkanoate (PHA) biopolymer is highly dependent on the activity of a key enzyme, PHA synthase (PhaC). The halophilic archaeon Haloferax mediterranei can accumulate large amounts of PHAs from different carbon sources under ...
Diya Alsafadi, Yomen Ghalawinji, Fawwaz I. Khalili   +2 more
doaj   +2 more sources

Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria [PDF]

Microbial Cell Factories
Polyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential.
Abdiqani Ibrahim Osman, Brendon Noble, Linda Percy, Pooja Basnett   +3 more
doaj   +2 more sources