Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes [PDF]
Microorganisms, 2022 Polyhydroxyalkanoate (PHA), a biodegradable and plastic-like biopolymer, has been receiving research and industrial attention due to severe plastic pollution, resource depletion, and global waste issues.
Radwa Moanis +2 more
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PHA synthase variant design using a conditional variational autoencoder. [PDF]
PLoS Computational BiologyPolyhydroxyalkanoate (PHA) synthases are a group of complex, dimeric enzymes which catalyze polymerization of R-hydroxyacids into PHAs. PHA properties depend on their monomer composition but enzymes found in nature have limited specificities to certain R-
Tuula Tenkanen +4 more
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Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition [PDF]
Brazilian Archives of Biology and Technology, 2013 In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa.
Abdul Basit Khan +6 more
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Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1Ps6-19) was commonly utilized in the de novo biosynthesis of PLA from biomass.
Mengxun Shi +8 more
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In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases [PDF]
FEMS Microbiology Letters, 2005 For the first time a functional protein was fused to a PHA synthase resulting in PHA granule formation and display of the respective function at the PHA granule surface. The GFP reporter protein was N-terminally fused to the class I PHA synthase of Cupriavidus necator (PhaC) and the class II PHA synthase of Pseudomonas aeruginosa PAO1 (PhaC1 ...
Peters, V, Rehm, BHA
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Applied Microbiology and Biotechnology, 2002 In vivo random mutagenesis of the polyhydroxyalkanoate (PHA) synthase gene from Aeromonas punctata was performed employing the mutator strain Escherichia coli XL1-Red. About 200,000 mutants were screened on Nile red-containing medium and five mutants with enhanced fluorescence were selected. Four of these mutants exhibited enhanced in vivo and in vitro
Amara, AA, Steinbuchel, A, Rehm, BHA
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Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the Extremophile Haloferax mediterranei [PDF]
Marine DrugsBioplastics such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are promising alternatives to conventional plastics. However, the high production cost limits their industrial application.
Alexandra Simica +4 more
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Microbial Cell Factories, 2012 Background Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate (3HB) monomers and medium-chain-
Gao Xue +7 more
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Heterologous Expression of Various PHA Synthase Genes in Rhodospirillum rubrum [PDF]
Chemical and Biochemical Engineering Quarterly, 2015 The phototrophic non-sulfur purple bacterium Rhodospirillum rubrum is known for its metabolic versatility. Particularly, R. rubrum is able to synthesize PHA under heterotrophic or even autotrophic growth with carbon monoxide as carbon and energy source. R. rubrum has therefore become a promising candidate for future cheap PHA production.
Klask, C. +3 more
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RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production [PDF]
Biomolecules, 2019 The inexhaustible nature and biodegradability of bioplastics like polyhydroxyalkanoates (PHAs) make them suitable assets to replace synthetic plastics. The eventual fate of these eco-friendly and non-toxic bioplastics relies upon the endeavors towards satisfying cost and, in addition, execution necessity.
Apoorva Rao +4 more
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