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Antibody discovery: phage display
Current Opinion in Biotechnology, 2002Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify a fully human therapeutic antibody
Titus Kretzschmar, Thomas von Rüden
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Combinatorial Chemistry & High Throughput Screening, 2012
Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phage mid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacterial genome.
Lars Frykberg, Karin Jacobsson
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Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phage mid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacterial genome.
Lars Frykberg, Karin Jacobsson
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Affinity Maturation by Phage Display
2008Antibodies are indispensable tools for research, diagnostics, and therapy. However, sometimes antibodies with the most favourable specificity profile lack sufficient affinity for a desired application. Here, we describe a method to increase the affinity of recombinant scFv antibody fragments based on random mutagenesis and phage display under stringent
Bernd Voedisch +4 more
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2018
Phage display is a powerful technique for studying protein–ligand interactions most frequently applied to protein–protein, protein–peptide, and protein–nucleic acids interactions. The genetic code for the protein/peptide of interest is inserted in the genome of a phage and subsequently “displayed” on the surface of the viral particle as a fusion to ...
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Phage display is a powerful technique for studying protein–ligand interactions most frequently applied to protein–protein, protein–peptide, and protein–nucleic acids interactions. The genetic code for the protein/peptide of interest is inserted in the genome of a phage and subsequently “displayed” on the surface of the viral particle as a fusion to ...
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Functional cloning by phage display
Biochimie, 2008This review focusses on the isolation of proteins from genomic or cDNA expression products libraries displayed on phage. The use of phage display is highlighted for the characterization of binding proteins with diverse biological functions. Phage display is compared with another strategy, the yeast two-hybrid method.
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Phage display in pharmaceutical biotechnology
Current Opinion in Biotechnology, 2000Over the past year, methods for the construction of M13 phage-display libraries have been significantly improved and new display formats have been developed. Phage-displayed peptide libraries have been used to isolate specific ligands for numerous protein targets.
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Phage display systems and their applications
Applied Microbiology and Biotechnology, 2006Screening phage display libraries of proteins and peptides has, for almost two decades, proven to be a powerful technology for selecting polypeptides with desired biological and physicochemical properties from huge molecular libraries. The scope of phage display applications continues to expand.
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Quantum Dots for Display Applications
Angewandte Chemie - International Edition, 2020Haiyan Qin, Xing Lin, Xiaogang Peng
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Large-area display textiles integrated with functional systems
Nature, 2021Peining Chen, Peng Zhai, Bingjie Wang
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