Results 211 to 220 of about 114,213 (298)

Insights into the catalytic mechanism of formate dehydrogenases from different microbial sources

The FEBS Journal, EarlyView.
This integrated study combines experimental enzyme kinetics with QM/MM simulations to map the catalytic mechanisms of four formate dehydrogenases at the atomic level. This approach reveals the key determinants of catalytic efficiency and guides the rational design of biocatalysts for effective CO2 reduction—a crucial step towards sustainable ...
Laura Legnani, Marco Gargiulo, Elia Lio, Enrico Mario Alessandro Fassi, Giovanni Grazioso, Maria Assunta Chiacchio, Barış Binay, Stefania Brocca, Francesco Secundo   +8 more
wiley   +1 more source

Staphylococcus aureus Sb‐1 Bacteriophage Alters Frequency and Subset Distribution of Human Innate Lymphoid Cells

Allergy, EarlyView.
Ramazan Rozumbetov, Ismail Ogulur, Anja Heider, Anna Globinska, Nina Chanishvili, Elene Kakabadze, Marina Goderdzishvili, Nino Grdzelishvili, Nata Bakuradze, Moelong Yu, Tamara Aripova, Adolat Ismailova, Nikolaos Papadopoulos, Mubeccel Akdis, Cezmi A. Akdis   +14 more
wiley   +1 more source

Gene Editing for Haemophilia—The Next Frontier

Haemophilia, EarlyView.
ABSTRACT The recently approved haemophilia A and B gene therapies via adeno‐associated virus (AAV) showed a promising therapeutic response after a single injection, but there are still limitations, including the potential loss of transgene expression and restriction in adults.
Mirko Pinotti, Gregory A. Newby, Sundar Selvaraj, Steven W. Pipe   +3 more
wiley   +1 more source

High-Throughput Identification and Characterization of LptDE-Binding Bicycle Peptides Using Phage Display and Cryo-EM. [PDF]

J Med Chem
Allyjaun S, Dunbar E, Hardwick SW, Newell S, Holding F, Rowland CE, St Denis MA, Pellegrino S, Arruda Bezerra G, Bournakas N, Chirgadze DY, Cooper L, Paris G, Lewis N, Brown P, Skynner MJ, Dawson MJ, Beswick P, Hubbard J, van den Berg B, Newman H.   +20 more
europepmc   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, EarlyView.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, EarlyView.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Defining short linear motif binding determinants by phage display-based deep mutational scanning. [PDF]

Protein Sci
Benz C, Maassen L, Simonetti L, Mihalic F, Lindqvist R, Tsitsa I, Konstantinou A, Jemth P, Överby AK, Davey NE, Ivarsson Y.   +10 more
europepmc   +1 more source

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, EarlyView.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Discovery of d‑Miniprotein Inhibitors of PD-1/PD-L1 Interaction via Mirror-Image Phage Display against Synthetic d‑PD‑1. [PDF]

JACS Au
Zhou H, Wu H, Zhong Z, Blasco P, Zeng J, Wang J, Liu H, Li X.   +7 more
europepmc   +1 more source