Results 201 to 210 of about 978,488 (287)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

In Vitro and Clinical Evaluation of the Anti-Wrinkle Efficacy of Medipep-6PN, a Novel Peptide Identified by Phage Display. [PDF]

Int J Mol Sci
Bang J, Im K, Hwang YL, Kim MY, Yun JN, Chang MY, Kim S, Lee JH.   +7 more
europepmc   +1 more source

Specific peptide enrichment strategies for neuropsychiatric disorders: From material design to diagnostic applications

Interdisciplinary Medicine, EarlyView.
This review outlines specific peptide enrichment strategies, including metal, magnetic oxides, functional polymers, and antibody‐based approaches, and their mechanisms, highlighting applications in biomarker discovery for major neurological and psychiatric disorders.
Xue Zhang, Yejiao Shi, Minghui Guo, Yang Huang, Yirong Wang, Leping Huang, Helena S. Azevedo, Honggang Hu, Xin Dong   +8 more
wiley   +1 more source

Identification of novel serum biomarkers for Alzheimer's disease screened by phage display library of random peptides. [PDF]

J Alzheimers Dis Rep
Gao D, Han Z, Ai X, Wang F, Lai D, Gao C, Zhang Z, Tao S, Shang J, Zhang J.   +9 more
europepmc   +1 more source

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli: Tuning inclusion body architecture and protein yield via dissolved oxygen control

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil, Norma A Valdez‐Cruz, Sara Restrepo‐Pineda, Nuria Sánchez‐Puig, Rafael Vázquez‐Duhalt, Rubén D Cadena‐Nava, Néstor O Pérez, Mauricio A Trujillo‐Roldán   +7 more
wiley   +1 more source

Isolation of neutralizing antibodies against SARS-CoV-2 through an epitope-guided negative screening by phage display. [PDF]

J Biomed Res
Lu M, Chen Y, Liu X, Gao F, Gou L, Ye W, Wen J, Guo X, Gao W.   +8 more
europepmc   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Using phage display for rational engineering of a higher-affinity humanized 3' phosphohistidine-specific antibody. [PDF]

Commun Chem
Martyn GD, Kalagiri R, Veggiani G, Stanfield RL, Choudhuri I, Sala M, Meisenhelder J, Chen C, Biswas A, Levy RM, Lyumkis D, Wilson IA, Hunter T, Sidhu SS.   +13 more
europepmc   +1 more source

Microbial communities and functional diversity in seafood

JSFA reports, EarlyView.
Abstract Functional diversity encompasses ecosystem processes that enhance adaptability to environmental change. This study explores the diversity of microorganisms associated with seafood. In this paper, we present our knowledge of microbial diversity in relation to seafood.
Christian Larbi Ayisi, Samuel Ayeh Osei, Grace Afumwaa Boamah, Gertrude Dzifa Mensah   +3 more
wiley   +1 more source

Use of a Bacteriophage Lysin to Control Axillary Malodor

JEADV Clinical Practice, EarlyView.
ABSTRACT Background Axillary malodor is primarily caused by bacteria, particularly Staphylococcus hominis, which metabolize apocrine secretions into volatile compounds. Traditional deodorants contain antiseptics that can disrupt the skin microbiome and cause irritation. Alternative methods of odor control are being sought but so far without success. We
Maurice A. M. van Steensel, Fabian Hia, Mei Zhen Beh, Boon Chong Goh   +3 more
wiley   +1 more source