The Organization and Function of the Phagophore-ER Membrane Contact Sites
Contact, 2023 Macroautophagy is characterized by the de novo formation of double-membrane vesicles termed autophagosomes. The precursor structure of autophagosomes is a membrane cistern called phagophore, which elongates through a massive acquisition of lipids until ...
Fulvio Reggiori
exaly +3 more sources
Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins [PDF]
Nature CommunicationsLysosome-targeting chimeras specifically degrade membrane/extracellular proteins with the specificity and potency highly dependent on the expression pattern and kinetics of lysosome-targeting receptors.
Pan Wang +9 more
doaj +3 more sources
ER-endosome contacts generate a local environment promoting phagophore formation
Cell ReportsSummary: Autophagy starts with the formation of a double-membrane vacuole called the autophagosome, initiated by a transient structure known as the phagophore. Previous studies reported that phagophore biogenesis primarily occurs at endoplasmic reticulum
Etienne Morel, Naima El Khallouki
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Morphology of Phagophore Precursors by Correlative Light-Electron Microscopy
Cells, 2022 Autophagosome biogenesis occurs in the transient subdomains of the endoplasmic reticulum that are called omegasomes, which, in fluorescence microscopy, appear as small puncta, which then grow in diameter and finally shrink and disappear once the ...
Sigurdur Runar Gudmundsson +2 more
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Atg2/TRAPPIII-Ypt1 axis: deciphering the phagophore-ERES connection [PDF]
AutophagyDe novo generation of membrane contact sites (MCSs) between the nascent phagophore and the endoplasmic reticulum (ER), particularly the ER exit sites (ERES), are crucial for autophagy as they provide the lipids necessary for the phagophore expansion into
Rubén Gómez-Sánchez +2 more
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Atg18 interaction positions Atg2 for efficient lipid transfer into phagophore elongation [PDF]
The EMBO JournalDuring macroautophagy, the de novo formation of the autophagosome at a membrane contact site (MCS) with the endoplasmic reticulum requires directional lipid flux for the growth of the initial phagophore before its sealing into an autophagosome and ...
Sabrina Chumpen Ramirez +10 more
doaj +3 more sources
TOM40 Targets Atg2 to Mitochondria-Associated ER Membranes for Phagophore Expansion
Cell Reports, 2019 Summary: During autophagy, phagophores grow into double-membrane vesicles called autophagosomes, but the underlying mechanism remains unclear. Here, we show a critical role of Atg2A in phagophore expansion.
Tatsuya Hattori +2 more
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The autophagy-specific exocyst subcomplex contributes to phagophore assembly site integrity by promoting phagophore expansion [PDF]
Proceedings of the National Academy of Sciences of the United States of AmericaSignificance This study significantly enhances our understanding of membrane expansion during autophagy by decoding the role of the tethering complex exocyst during autophagosome biogenesis.
Ravi R Manjithaya +2 more
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A GABARAP−PtdIns3K-C1 positive feedback loop at the heart of the phagophore nucleation [PDF]
Nature CommunicationsIn mammalian cells, autophagosomes can reach diameters of over 1000 nm within 30 min after triggering starvation, but how such substantial amounts of membranes can be synthesized remains elusive.
Antoine N. Dessus +7 more
doaj +3 more sources
Kenny is the adaptor protein for ubiquitin-dependent mitophagy in Drosophila melanogaster [PDF]
Autophagy ReportsMitophagy is the selective degradation program for damaged and unnecessary mitochondria to maintain cellular mitostasis and survival. Specific mutations in the mediators for the canonical ubiquitin (ub)-dependent mitophagy pathway have been identified ...
Hubert Osei Acheampong, Ryan Insolera
doaj +2 more sources