The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy [PDF]
Autophagy, 2021 To resolve the signaling mechanisms that mediate the starvation-induced processes of Dictyostelium sporulation and encystation, we performed insertional mutagenesis on cells harboring an mRFP-tagged spore gene.
Yoko Yamada, Pauline Schaap
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Kenny is the adaptor protein for ubiquitin-dependent mitophagy in Drosophila melanogaster [PDF]
Autophagy ReportsMitophagy is the selective degradation program for damaged and unnecessary mitochondria to maintain cellular mitostasis and survival. Specific mutations in the mediators for the canonical ubiquitin (ub)-dependent mitophagy pathway have been identified ...
Hubert Osei Acheampong, Ryan Insolera
doaj +2 more sources
Morphology of Phagophore Precursors by Correlative Light-Electron Microscopy
Cells, 2022 Autophagosome biogenesis occurs in the transient subdomains of the endoplasmic reticulum that are called omegasomes, which, in fluorescence microscopy, appear as small puncta, which then grow in diameter and finally shrink and disappear once the ...
Sigurdur Runar Gudmundsson +2 more
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Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins [PDF]
Nature CommunicationsLysosome-targeting chimeras specifically degrade membrane/extracellular proteins with the specificity and potency highly dependent on the expression pattern and kinetics of lysosome-targeting receptors.
Pan Wang +9 more
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TOM40 Targets Atg2 to Mitochondria-Associated ER Membranes for Phagophore Expansion
Cell Reports, 2019 Summary: During autophagy, phagophores grow into double-membrane vesicles called autophagosomes, but the underlying mechanism remains unclear. Here, we show a critical role of Atg2A in phagophore expansion.
Zhenyuan Tang +2 more
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ATG9A-dependent, LC3-independent autophagy curbs the immune system to protect against disease [PDF]
Autophagy ReportsSelective autophagy is generally believed to require the conjugation of microtubule associated protein 1 light chain 3 (LC3) proteins (or other autophagy-related 8 [ATG8] family members) on the inner phagophore leaflet to enable the recruitment of cargo ...
Dario Priem, Mathieu JM Bertrand
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ER-endosome contacts generate a local environment promoting phagophore formation
Cell ReportsSummary: Autophagy starts with the formation of a double-membrane vacuole called the autophagosome, initiated by a transient structure known as the phagophore. Previous studies reported that phagophore biogenesis primarily occurs at endoplasmic reticulum
Etienne MOREL, Francesca Giordano
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An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure
Nature Communications, 2018 During autophagy, phagophores elongate to form double-membrane vesicles but the mechanism behind their closure is unknown. Here, the authors develop an autophagy assay and find a role for the endosomal sorting complexes required for transport component ...
Yoshinori Takahashi +2 more
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Two specific interactions of GATE16 with TRPML3 and RAB33B regulate autophagy [PDF]
Scientific ReportsATG8s are essential for autophagy as they recruit various machinery to autophagic structures. We previously reported that the intracellular Ca2+ channel TRPML3 specifically interacts with the mammalian ATG8 homolog GATE16, but not LC3B to increase ...
Jiwoo Park +5 more
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Communicative and Integrative Biology, 2018 Autophagy is an essential physiological process that maintains cellular homeostasis by eliminating harmful protein aggregates, damaged organelles and certain pathogens through lysosomal degradation.
Shivranjani Moharir, Ghanshyam Swarup
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