Results 11 to 20 of about 13,993 (208)

How Membrane Contact Sites Shape the Phagophore

Contact, 2023
During macroautophagy, phagophores establish multiple membrane contact sites (MCSs) with other organelles that are pivotal for proper phagophore assembly and growth. In S.
Cristina Capitanio, Anna Bieber, Florian Wilfling   +2 more
doaj   +4 more sources

COPII vesicles and the expansion of the phagophore

eLife, 2019
A new study has identified the proteins that adapt COPII vesicles to the needs of starving cells.
Catherine Rabouille
doaj   +5 more sources

The Organization and Function of the Phagophore-ER Membrane Contact Sites

Contact, 2023
Macroautophagy is characterized by the de novo formation of double-membrane vesicles termed autophagosomes. The precursor structure of autophagosomes is a membrane cistern called phagophore, which elongates through a massive acquisition of lipids until ...
Prado Vargas Duarte, Fulvio Reggiori
doaj   +4 more sources

Hepatitis C Virus NS5A Activates Mitophagy Through Cargo Receptor and Phagophore Formation

Pathogens
Chronic HCV infection is a risk factor for end-stage liver disease, leading to a major burden on public health. Mitophagy is a specific form of selective autophagy that eliminates mitochondria to maintain mitochondrial integrity.
Yuan-Chao Hsiao, Chih-Wei Chang, Chau-Ting Yeh, Po-Yuan Ke   +3 more
doaj   +3 more sources

Establishment of the phagophore-ERES membrane contact site initiates phagophore elongation. [PDF]

Nat Struct Mol Biol
The de novo generation of membrane contact sites (MCSs) between the phagophore and the endoplasmic reticulum exit sites (ERES) is important for the acquisition of the lipids necessary for phagophore elongation and autophagosome formation during autophagy. However, it is currently unclear how these MCSs are established.
Gómez-Sánchez R, Chumpen Ramirez S, Vargas Duarte P, Hu Y, Mari M, Olschewski K, Hardenberg R, Fromme JC, Ungermann C, Reggiori F.   +9 more
europepmc   +3 more sources

Mammalian autophagosomes form from finger-like phagophores

Developmental Cell, 2023
The sequence of morphological intermediates that leads to mammalian autophagosome formation and closure is a crucial yet poorly understood issue. Previous studies have shown that yeast autophagosomes evolve from cup-shaped phagophores with only one closure point, and mammalian studies have inferred that mammalian phagophores also have single openings ...
Claudia Puri, Matthew J. Gratian, David C. Rubinsztein   +2 more
openaire   +3 more sources

The autophagy-specific exocyst subcomplex contributes to phagophore assembly site integrity by promoting phagophore expansion. [PDF]

Proc Natl Acad Sci U S A
Autophagy is a crucial intracellular pathway for maintaining cellular homeostasis. It involves forming double-membrane vesicles called autophagosomes, which deliver cytosolic cargoes to the lysosomes/vacuoles for degradation. Biogenesis of autophagosomes is a membrane-intensive process wherein the membrane expansion steps are poorly understood.
Kumari R, Nath T, Pflaum J, R I, Vinay S, Wilfling F, Manjithaya R.   +6 more
europepmc   +3 more sources

Sulforaphanes: disruptors of phagophores and autolysosomes

Autophagy Reports, 2022
Sulforaphane and its metabolites (SFNs) cause apoptosis in cancers and could be potential anti-cancer drugs. We focused on investigating the underlying mechanisms through which SFNs inhibit cancers. First, SFNs cause microtubule disruption by phosphorylated MAPK1/ERK2-MAPK3/ERK1-mediated activation of 26S proteasome leading to a microtubule-associated ...
Yan Zhou, Wei Wu
openaire   +3 more sources

Phagophores evolve from recycling endosomes [PDF]

Autophagy, 2018
The membrane origins of autophagosomes have been a key unresolved question in the field. The earliest morphologically recognizable structure in the macroautophagy/autophagy itinerary is the double-membraned cup-shaped phagophore. Newly formed phosphatidylinositol 3-phosphate (PtdIns3P) on the membranes destined to become phagophores recruits WIPI2 ...
Puri, Claudia, Vicinanza, Mariella, Rubinsztein, David C   +2 more
openaire   +2 more sources

ESCRT-mediated phagophore sealing during mitophagy [PDF]

Autophagy, 2019
Inactivation of the endosomal sorting complex required for transport (ESCRT) machinery has been reported to cause autophagic defects, but the exact functions of ESCRT proteins in macroautophagy/autophagy remain incompletely understood. Using live-cell fluorescence microscopy we found that the filament-forming ESCRT-III subunit CHMP4B was recruited ...
Zhen, Yan, Spangenberg, Hélène, Munson, Michael J., Brech, Andreas, Schink, Kay O., Tan, Kia-Wee, Sørensen, Vigdis, Wenzel, Eva Maria, Radulovic, Maja, Engedal, Nikolai, Simonsen, Anne, Raiborg, Camilla, Stenmark, Harald   +12 more
openaire   +4 more sources