Results 71 to 80 of about 5,655,447 (304)

Novel liquid phase separation techniques for proteome study

, 2008
Novel liquid phase separation techniques for proteome ...
张玉奎
core  

Phase separation with nonlocal interactions

Physical Review Research
Phase separation in complex systems is a ubiquitous phenomenon. While simple theories predict coarsening until only macroscopically large phases remain, concrete models often exhibit patterns with finite length scales.
Filipe C. Thewes, Yicheng Qiang, Oliver W. Paulin, David Zwicker   +3 more
doaj   +1 more source

Understanding how genetically encoded tags and crowding agents affect phase separation by heterochromatin protein HP1α

Cell Reports: Methods
Summary: The heterochromatin protein HP1α (heterochromatin protein 1 alpha) phase separates in vitro and displays properties compatible with phase separation in cells.
Ziling (Kate) Zhou, Kibeom Hong, Bo Huang, Geeta J. Narlikar   +3 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

, 2011
Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteinsBoth of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein ...
Gai, Qingqing, Qu, Feng, 张玉奎, Zhang, Yukui, 屈锋, Zhang, Tao   +5 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

CFD Instraction Guide to Simulate Two-Phase Flow Separation in A Vertical T-Junction Separator

, 2019
Two-phase flow separation has been investigated by many researchers due to its important role in the most industrial applications. In refrigeration and air conditioning systems, two-phase flow separation influence the system performance.
Mahmood, Raid Ahmed
core  

Separation behavior of basic compounds on unbonded silicon oxynitride and silica high-performance liquid chromatography stationary phases with reversed-phase eluents

, 2016
Unbonded silicon oxynitride and silica high-performance liquid chromatography stationary phases have been evaluated and compared for the separation of basic compounds of differing molecular weight, pKa, and log D using aqueous/organic mobile phases.
Xue, Xingya, Wan, Huihui, Liang, Xinmiao, Zhong, Hongmin   +3 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source